The effects of mepyramine maleate, sodium meclofenamate, methysergide bimaleate, diethylcarbamazine citrate, and disodium cromoglycate on bovine cutaneous anaphylactic reactions and responses to intradermal injections of histamine, 5-hydroxytryptamine, and bradykinin were studied. Calves sensitized by immunization with horse serum in Freund's complete adjuvant were bled to obtain skin-sensitizing serum which was used in passive cutaneous anaphylaxis (P.C.A.) tests. A latent period of 72 h was allowed between intradermal injections of sera and antigen challenge. Mepyramine maleate (10 mg/kg) selectively inhibited responses to histamine and raised the threshold dose of antibody required to elicit a response. Disodium cromoglycate (DSCG, 10 mg/kg) given intravenously 30 min or immediately prior to challenge had no significant effect either on the responses to drugs or on the passive cutaneous anaphylaxis reaction. Diethylcarbamazine (DECC, 20 mg/kg) administered immediately prior to challenge significantly inhibited the cutaneous anaphylactic response and when DSCG was given simultaneously with DECC, this inhibitory effect was apparently augmented. Methysergide (1 mg/kg) and sodium meclofenamate (2 mg/kg) were ineffective in suppressing P.C.A. It is concluded that histamine and a slow-reacting substance (SRS-A) are involved in the mediation of bovine cutaneous anaphylaxis and that kinins may contribute, while 5-hydroxytryptamine would appear unimportant.
Inhibitory Effect of Schizandrin on Passive Cutaneous Anaphylaxis Reaction and Scratching Behaviors in Mice