Adjuvant effect of Photobacterium phosphoreum PJ-1 on humoral immune response of ddY mice to sheep erythrocytes.

The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to recombinant BCG (rBCG) vaccination was evaluated. Isogenic mice were immunized with rBCG expressing the R1 repeat region of the P97 adhesin of Mycoplasma hyopneumoniae alone (rBCG/R1) or fused to LTB (rBCG/LTBR1). Anti-R1 systemic antibody levels (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) were measured by ELISA using recombinant R1 as antigen. With the exception of IgM, LTB doubled the anti-R1 antibody levels in rBCG vaccination. The IgG1/IgG2a mean ratio showed that both rBCG/LTBR1 and rBCG/R1 induced a mixed Th1/Th2 immune response. Interestingly, anti-R1 serum IgA was induced only by rBCG/LTBR1. These results demonstrate that LTB has an adjuvant effect on the humoral immune response to recombinant antigens expressed in BCG.

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Role of Complement Receptor Type 2 and Endogenous Complement in the Humoral Immune Response to Conjugates of Complement C3d and Pneumococcal Serotype 14 Capsular Polysaccharide

Infection and Immunity ◽

10.1128/iai.73.11.7311-7316.2005 ◽

2005 ◽

Vol 73 (11) ◽

pp. 7311-7316 ◽

Cited By ~ 6

Author(s):

Joyce K. Mitsuyoshi ◽

Yong Hu ◽

Samuel T. Test

Keyword(s):

Immune Response ◽

T Cell ◽

Antibody Response ◽

Humoral Immune Response ◽

Capsular Polysaccharide ◽

Complement Receptor ◽

Adjuvant Effect ◽

Humoral Immune

ABSTRACT Conjugation of the complement fragment C3d to both T-cell-dependent (TD) protein and T-cell-independent type 2 (TI-2) polysaccharide antigens enhances the humoral immune response in mice immunized with either type of antigen. However, the ability of C3d-protein conjugates to enhance the antibody response in mice deficient in complement receptor types 1 and 2 (CR1 and CR2) has raised questions about the role of C3d-CR2 interactions in the adjuvant effect of C3d. In this study, we examined the role of CR2 binding and endogenous complement activation in the antibody response to conjugates of C3d and serotype 14 pneumococcal capsular polysaccharide (PPS14). To block binding of PPS14-C3d conjugates to CR2, mice were immunized with a mixture of vaccine and (CR2)2-immunoglobulin G1 (IgG1). Mice receiving (CR2)2-IgG1 at the time of primary immunization had a marked reduction in the primary anti-PPS14 antibody response but an enhanced secondary anti-PPS14 response, suggesting that C3d-CR2 interactions are required for the primary response but can have negative effects on the memory response. Further, compared with mice receiving PPS14-C3d having a high C3d/PPS14 ratio, mice immunized with PPS14-C3d with low C3d/PPS14 ratios had an enhanced secondary antibody response. Treatment of mice with cobra venom factor to deplete complement had insignificant effects on the antibody response to PPS14-C3d. Experiments with CBA/N xid mice confirmed that PPS14-C3d conjugates retain the characteristics of TI-2 rather than TD antigens. Thus, the adjuvant effect of C3d conjugated to PPS14 requires C3d-CR2 interactions, does not require activation of endogenous complement, and is not mediated by TD carrier effects.

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The adjuvant effect of jacalin on the mouse humoral immune response to trinitrophenyl and Trypanosoma cruzi

Immunology Letters ◽

10.1016/s0165-2478(99)00079-6 ◽

1999 ◽

Vol 68 (2-3) ◽

pp. 375-381 ◽

Cited By ~ 12

Author(s):

Deijanira A Albuquerque ◽

Gislâine A Martins ◽

Antônio Campos-Neto ◽

João S Silva

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Humoral Immune Response ◽

Adjuvant Effect ◽

Humoral Immune

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Effects of route of immunization, adjuvant and unrelated antigens on the humoral immune response in lines of chickens selected for antibody production against sheep erythrocytes

Veterinary Immunology and Immunopathology ◽

10.1016/0165-2427(92)90039-s ◽

1992 ◽

Vol 33 (1-2) ◽

pp. 115-127 ◽

Cited By ~ 31

Author(s):

M.B. Kreukniet ◽

A.J. van der Zijpp ◽

M.G.B. Nieuwland

Keyword(s):

Immune Response ◽

Antibody Production ◽

Humoral Immune Response ◽

Sheep Erythrocytes ◽

Humoral Immune

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Genetic and phenotypic aspects of the interrelationship between cellular and humoral immune response to sheep erythrocytes in mice

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00836425 ◽

1993 ◽

Vol 115 (3) ◽

pp. 315-316

Author(s):

E. Yu. Gusev ◽

N. N. Kevorkov

Keyword(s):

Immune Response ◽

Humoral Immune Response ◽

Sheep Erythrocytes ◽

Humoral Immune

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Potentiation of the humoral immune response elicited by a commercial vaccine against bovine respiratory disease by Enterococcus faecalis CECT7121

Beneficial Microbes ◽

10.3920/bm2017.0081 ◽

2018 ◽

Vol 9 (4) ◽

pp. 553-562 ◽

Cited By ~ 2

Author(s):

A.M. Díaz ◽

B. Almozni ◽

M.A. Molina ◽

M.D. Sparo ◽

M.A. Manghi ◽

...

Keyword(s):

Immune Response ◽

Respiratory Disease ◽

Enterococcus Faecalis ◽

Humoral Immune Response ◽

Pasteurella Multocida ◽

Bovine Respiratory Disease ◽

Intragastric Administration ◽

Adjuvant Effect ◽

Humoral Immune ◽

Commercial Vaccine

Vaccination against pathogens involved in bovine respiratory disease (BRD) is a useful tool to reduce the risk of this disease however, it has been observed that the commercially available vaccines only partially prevent the infections caused by Pasteurella multocida and Mannheimia haemolytica. Therefore, it is recommended to search for new adjuvant strategies to minimise the economic impact of this respiratory syndrome. A possibility to improve the conventional vaccine response is to modulate the immune system with probiotics, since there is accumulating evidence that certain immunomodulatory strains administered around the time of vaccination can potentiate the immune response. Considering veterinary vaccines are frequently tested in murine models, we have developed an immunisation schedule in BALB/c mice that allows us to study the immune response elicited by BRD vaccine. In order to evaluate a potential strategy to enhance vaccine efficacy, the adjuvant effect of Enterococcus faecalis CECT7121 on the murine specific humoral immune response elicited by a commercial vaccine against BRD was studied. Results indicate that the intragastric administration of E. faecalis CECT7121 was able to induce an increase in the specific antibody titres against the bacterial components of the BRD vaccines (P. multocida and M. haemolytica). The quality of the humoral immune response, in terms of antibody avidity, was also improved. Regarding the cellular immune response, although the BRD vaccination induced a low specific secretion of cytokines in the spleen cell culture supernatants, E. faecalis CECT7121-treated mice showed higher interferon-γ production than immunised control mice. Our results allowed us to conclude that the administration of E. faecalis CECT7121 could be employed as an adjuvant strategy to potentiate humoral immune responses.

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