Single Muscle Fiber Adaptations to Resistance training in the Oldest-Old Women

2006 ◽  
Vol 38 (Supplement) ◽  
pp. S276
Author(s):  
Dustin Slivka ◽  
Ulrika Raue ◽  
Kiril Minchev ◽  
Scott Trappe
Keyword(s):  
Resistance Training ◽  
Muscle Fiber ◽  
Oldest Old ◽  
Single Muscle ◽  
Single Muscle Fiber

Effects of Short-Term Concentric vs. Eccentric Resistance Training on Single Muscle Fiber MHC Distribution in Humans

2005 ◽  
Vol 26 (5) ◽  
pp. 339-343 ◽  
Author(s):  
U. Raue ◽  
B. Terpstra ◽  
D. L. Williamson ◽  
P. M. Gallagher ◽  
S. W. Trappe
Keyword(s):  
Resistance Training ◽  
Muscle Fiber ◽  
Single Muscle ◽  
Short Term ◽  
Single Muscle Fiber

scholarly journals Single muscle fiber gene expression with resistance training in young women

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Ulrika Raue ◽  
Travis Conley ◽  
Emily Louis ◽  
Kiril Minchev ◽  
Bozena Jemiolo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Training ◽  
Young Women ◽  
Muscle Fiber ◽  
Single Muscle ◽  
Single Muscle Fiber ◽  
Fiber Gene

scholarly journals Reduction in hybrid single muscle fiber proportions with resistance training in humans

2001 ◽  
Vol 91 (5) ◽  
pp. 1955-1961 ◽  
Author(s):  
D. L. Williamson ◽  
P. M. Gallagher ◽  
C. C. Carroll ◽  
U. Raue ◽  
S. W. Trappe
Keyword(s):  
Resistance Training ◽  
Muscle Fiber ◽  
Vastus Lateralis ◽  
Electrophoretic Analysis ◽  
Training Data ◽  
Single Muscle ◽  
Hybrid Fibers ◽  
Mhc I ◽  
Single Muscle Fiber ◽  
Mhc Iia

The purpose of this investigation was to examine the effects of 12 wk of progressive resistance training (PRT) on single muscle fiber myosin heavy chain (MHC; I, I/IIa, I/IIa/IIx, IIa, IIa/IIx, IIx) isoform proportions in young individuals. Young, untrained men (YM; n = 6) and women (YW; n = 6) (age = 22 ± 1 and 25 ± 2 yr for YW and YM, respectively) received pre- and post-PRT muscle biopsies from the right vastus lateralis for single muscle fiber MHC distribution by electrophoretic analysis (192 ± 5 pre- and 183 ± 6 post-fibers/subject analyzed; 4,495 fibers total). Data are presented as percentages of the total fibers analyzed per subject. The PRT protocol elicited an increase in the pure MHC IIa (Δ = + 24 and + 27; YW and YM, respectively; P < 0.05) with no change in the pure MHC I distribution. The hybrid MHC distributions decreased I/IIa/IIx (Δ = −2; YM and YW; P < 0.05), IIa/IIx (Δ = −13 and −19 for YM and YW, respectively; P < 0.05), and total hybrid fiber proportion (I/IIa + I/IIa/IIx + IIa/IIx) decreased (Δ = −19 and −30 for YM and YW, respectively; P < 0.05) with the training, as did the MHC IIx distribution (Δ = −2; YW only; P < 0.05). Alterations in the predominance of MHC isoforms within hybrid fibers (decrease in MHC I-dominant I/IIa and nondominant MHC IIa/IIx, increase in MHC IIa-dominant IIa/IIx; P < 0.05) appeared to contribute to the increase in the MHC IIa proportion. Electrophoresis of muscle cross sections revealed an ∼7% increase ( P< 0.05) in MHC IIa proportion in both groups, whereas the MHC IIx decrease by 7.5 and 11.6% post-PRT in YW and YM, respectively. MHC I proportions increase in YM by 4.8% ( P < 0.05) post-PRT. These findings further support previous resistance training data in young adults with respect to the increase in the MHC IIa proportions but demonstrate that a majority of the change can be attributed to the decrease in single-fiber hybrid proportions.

EFFECTS OF CONCENTRIC VS. ECCENTRIC RESISTANCE TRAINING ON SINGLE MUSCLE FIBER MHC DISTRIBUTION IN YOUNG MEN

2002 ◽  
Vol 34 (5) ◽  
pp. S121
Author(s):  
U Raue ◽  
B Terpstra ◽  
D L. Williamson ◽  
P M. Gallagher ◽  
S W. Trappe
Keyword(s):  
Resistance Training ◽  
Muscle Fiber ◽  
Young Men ◽  
Single Muscle ◽  
Single Muscle Fiber

scholarly journals Improved single muscle fiber quality in the oldest-old

2016 ◽  
Vol 121 (4) ◽  
pp. 878-884 ◽  
Author(s):  
Greg J. Grosicki ◽  
Robert A. Standley ◽  
Kevin A. Murach ◽  
Ulrika Raue ◽  
Kiril Minchev ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Fiber Quality ◽  
Oldest Old ◽  
Muscle Fibers ◽  
Muscle Quality ◽  
Vastus Lateralis Muscle ◽  
Single Muscle ◽  
Mhc I ◽  
Single Muscle Fiber ◽  
Mhc Iia

We examined single muscle fiber contractile function of the oldest-old (3F/2M, 89 ± 1 yr old) enrolled in The Health, Aging, and Body Composition Study (The Health ABC Study). Vastus lateralis muscle biopsies were obtained and single muscle fiber function was determined ( n = 105) prior to myosin heavy chain (MHC) isoform identification with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cross-sectional area of MHC I muscle fibers (5,576 ± 333 μm2; n = 58) was 21% larger ( P < 0.05) than MHC IIa fibers (4,518 ± 386 μm2; n = 47). Normalized power (an indicator of muscle fiber quality incorporating size, strength, and speed) of MHC I and IIa muscle fibers was 2.3 ± 0.1 and 17.4 ± 0.8 W/l, respectively. Compared with previous research from our lab using identical procedures, MHC I normalized power was 28% higher than healthy 20 yr olds and similar to younger octogenarians (∼80 yr old). Normalized power of MHC IIa fibers was 63% greater than 20 yr olds and 39% greater than younger octogenarians. These comparative data suggest that power output per unit size (i.e., muscle quality) of remaining muscle fibers improves with age, a phenomenon more pronounced in MHC IIa fibers. Age-related single muscle fiber quality improvements may be a compensatory mechanism to help offset decrements in whole muscle function.

Single Muscle Fiber Characteristics of The Oldest-Old

2015 ◽  
Vol 47 ◽  
pp. 849
Author(s):  
Gregory Grosicki ◽  
Robert Standley ◽  
Kevin Murach ◽  
Ulrika Raue ◽  
Kiril Minchev ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Oldest Old ◽  
Single Muscle ◽  
Single Muscle Fiber

scholarly journals Single muscle fiber adaptations to resistance training in old (>80 yr) men: evidence for limited skeletal muscle plasticity

2008 ◽  
Vol 295 (1) ◽  
pp. R273-R280 ◽  
Author(s):  
Dustin Slivka ◽  
Ulrika Raue ◽  
Chris Hollon ◽  
Kiril Minchev ◽  
Scott Trappe
Keyword(s):  
Resistance Training ◽  
Muscle Fiber ◽  
Single Muscle ◽  
Very Old ◽  
Repetition Maximum ◽  
Muscle Plasticity ◽  
Single Muscle Fiber ◽  
Before And After ◽  
Old Men ◽  
Whole Muscle

The purpose of this study was to investigate whole muscle and single muscle fiber adaptations in very old men in response to progressive resistance training (PRT). Six healthy independently living old men (82 ± 1 yr; range 80–86 yr, 74 ± 4 kg) resistance-trained the knee extensors (3 sets, 10 repetitions) at ∼70% one repetition maximum 3 days/wk for 12 wk. Whole thigh muscle cross-sectional area (CSA) was assessed before and after PRT using computed tomography (CT). Muscle biopsies were obtained from the vastus lateralis before and after the PRT program. Isolated myosin heavy chain (MHC) I and IIa single muscle fibers ( n = 267; 142 pre; 125 post) were studied for diameter, peak tension, shortening velocity, and power. An additional set of isolated single muscle fibers ( n = 2,215; 1,202 pre; 1,013 post) was used to identify MHC distribution. One repetition maximum knee extensor strength increased ( P < 0.05) 23 ± 4 kg (56 ± 4 to 79 ± 7 kg; 41%). Muscle CSA increased ( P < 0.05) 3 ± 1 cm2 (120 ± 7 to 123 ± 7 cm2; 2.5%). Single muscle fiber contractile function and MHC distribution were unaltered with PRT. These data indicate limited muscle plasticity at the single-muscle fiber level with a resistance-training program among the very old. The minor increases in whole muscle CSA coupled with the static nature of the myocellular profile indicate that the strength gains were primarily neurological. These data contrast typical muscle responses to resistance training in young (∼20 yr) and old (∼70 yr) humans and indicate that the physiological regulation of muscle remodeling is adversely modified in the oldest old.

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