Primary Structure Details of Haptoglobin α Chain Proteins from Human Plasma Samples are Resolved by Matrix-Assisted Laser Desorption/Ionization Quadrupole Ion Trap Time-of-Flight Multiple-Stage Tandem Mass Spectrometry Sequencing

2004 ◽  
Vol 10 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Cornelia Koy ◽  
Martin Resch ◽  
Koichi Tanaka ◽  
Michael O. Glocker
Keyword(s):  
Mass Spectrometry ◽  
Ion Trap ◽  
Laser Desorption ◽  
Quadrupole Ion Trap ◽  
Tandem Mass ◽  
Plasma Samples ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Characterization and identification of disintegrins in Crotalus horridus venom by liquid chromatography and tandem matrix-assisted laser desorption ionization - quadrupole ion trap time-of-flight (MALDI-QIT-TOF) mass spectrometry

2005 ◽  
Vol 83 (8) ◽  
pp. 1124-1131 ◽  
Author(s):  
Jacob A Galán ◽  
Elda E Sánchez ◽  
Sajid Bashir ◽  
John C Pérez
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Trap ◽  
Laser Desorption ◽  
Quadrupole Ion Trap ◽  
Laser Desorption Ionization ◽  
Crotalus Horridus ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Two disintegrins were purified from the venom of Crotalus horridus by multiple-dimension liquid chromatography (MDLC) (specifically C18 reverse phase (RP) high-performance liquid chromatography followed by size exclusion chromatography (SEC), and anion exchange chromatography (SCX)). Both disintegrins were subjected to mass spectrometry to determine their intact molecular mass, the number of disulfide linkages, and protein sequence, respectively. These disintegrins were named horrdistatin 1 (nominal mass, 7231 Da) and horrdistatin 2 (nominal mass, 7451 Da), and had IC50 (inhibitory concentrations) of 12.5 and 16.2 nmol/L (at 50%), respectively. For sequence confirmation from the C-terminal end, both disintegrins were derivatized using chemical-assisted fragmentation (CAF) and subsequently unzipped via collision-induced dissociation (CID) by matrix-assisted laser desorption – ionization – quadrupole ion trap time-of-flight (MALDI-QIT-TOF) mass spectrometry.Key words: disintegrins, mass spectrometry, snake venom, Timber rattlesnake, Crotalus horridus.

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