scholarly journals The indirect india-ink immunoreaction for detection of antibodies to Encephalitozoon cuniculi in rat and mouse serum

1980 ◽  
Vol 14 (2) ◽  
pp. 83-86 ◽  
Author(s):  
B. S. Kellett ◽  
J. E. C. Bywater
Keyword(s):  
Diagnostic Test ◽  
Antibody Test ◽  
Mouse Serum ◽  
Indirect Fluorescence Antibody Test ◽  
Encephalitozoon Cuniculi ◽  
India Ink ◽  
Fluorescence Antibody ◽  
Indirect Fluorescence Antibody

The indirect india-ink immunoreaction and the india-ink immunoreaction inhibition tests are described and results are compared with those obtained by the indirect fluorescence antibody test. Titrations of seropositive samples were made by 2 methods and the titres obtained were similar. Some advantages of the india-ink immunoreaction as a diagnostic test are discussed.

scholarly journals Exposure of Domestic Cats to Distinct Ehrlichia canis TRP Genotypes

2021 ◽  
Vol 8 (12) ◽  
pp. 310
Author(s):  
Ísis Assis Braga ◽  
Isis Indaiara Gonçalves Granjeiro Taques ◽  
Estefânia Crivelatti Grontoski ◽  
Ingrid Savino de Oliveira Dias ◽  
Nathalia Assis Pereira ◽  
...  
Keyword(s):  
Antibody Test ◽  
Ehrlichia Canis ◽  
Indirect Fluorescence Antibody Test ◽  
Domestic Cats ◽  
Serum Samples ◽  
Specific Antibodies ◽  
First Report ◽  
The World ◽  
Fluorescence Antibody ◽  
Indirect Fluorescence Antibody

Cats naturally exposed to Ehrlichia canis have been described in different regions of the world, but little is known about the genotypes associated with infection in these animals. To detect E. canis-specific antibodies and investigate the E. canis TRP genotypes in cats, serum samples from 76 domestic cats reactive to crude E. canis antigens by the indirect fluorescence antibody test (IFAT) were analyzed by ELISA, using E. canis-specific peptides (i.e., TRP19 and TRP36 /BR/US/CR). Of these, 25 (32.9%) cats reacted to at least one TRP peptide, confirming their specific exposure to E. canis. Eighteen (23.7%) cats reacted to TRP19, 15 (19.8%) to BRTRP36, and 11 (14.5%) to USTRP36, but none of them reacted to CRTRP36. Eight (10.5%) cats reacted to TRP19 but not to any TRP36 genotype, demonstrating the possible existence of a new E. canis genotype infecting felines. Nevertheless, this study provides the first report of anti-E. canis-specific antibodies in domestic cats.

scholarly journals Determination of Anti-Bartonella henselae Antibody by Indirect Fluorescence Antibody Test

1998 ◽  
Vol 72 (8) ◽  
pp. 801-807 ◽  
Author(s):  
Hidehiro TSUNEOKA ◽  
Reiko FUJII ◽  
Kiyomi YAMAMOTO ◽  
Keiko FUJISAWA ◽  
Hidechika IINO ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Antibody Test ◽  
Indirect Fluorescence Antibody Test ◽  
Fluorescence Antibody ◽  
Indirect Fluorescence Antibody

scholarly journals Association between Neospora caninum seropositivity and reproductive disorders in girolando cows

2020 ◽  
Vol 72 (6) ◽  
pp. 2069-2076
Author(s):  
V.G. Gaia ◽  
G.F. Grillo ◽  
M.R.B. Mello ◽  
H.B. Palhano ◽  
A.F. Silva
Keyword(s):  
Dairy Cows ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Fisher Exact Test ◽  
Indirect Fluorescence Antibody Test ◽  
Reproductive Disorders ◽  
Blood Samples ◽  
Exact Test ◽  
Clinical Observations ◽  
Indirect Fluorescence Antibody

ABSTRACT The aim of this study was to investigate the presence of anti-Neospora caninum antibodies in Girolando cows, in order to evaluate the association between seropositivity and reproductive disorders. Blood samples were collected from 40 dairy cows in their reproductive phase from the cranial superficial epigastric vein. The blood samples were tested using the Indirect Fluorescence Antibody Test (IFAT) to detect anti-N.caninum antibodies. The serological results were used to verify whether there was any association with the manifestation of reproductive disorders based on data from the records of reproductive history from 2017 to 2018 as well as the clinical observations of the herd throughout this study. The Fisher exact test was used to verify the existence of an association between the serology and reproductive disorders, adopting a 95% confidence level. The serological results showed a 27.5% seroprevalence in the herd for N. caninum, however, after statistical analysis, no association between seropositivity and reproductive disorders was found in the evaluated herd. Although the studied population is infected with Neospora caninum, we can infer that anti-Neospora caninum antibodies present in Girolando dairy cows at the UFRRJ Dairy Cattle Facility are not associated with the occurrence of reproductive disorders.

scholarly journals Cytoplasmic, Nuclear, and Platelet Autoantibodies in Human Granulocytic Ehrlichiosis Patients

1998 ◽  
Vol 36 (7) ◽  
pp. 1959-1963 ◽  
Author(s):  
Susan J. Wong ◽  
Josephine A. Thomas
Keyword(s):  
Rheumatoid Factor ◽  
Antibody Test ◽  
Indirect Fluorescence Antibody Test ◽  
Serum Samples ◽  
Granulocytic Ehrlichiosis ◽  
Antiplatelet Antibodies ◽  
Human Granulocytic Ehrlichiosis ◽  
Spindle Apparatus ◽  
Indirect Fluorescence Antibody ◽  
Platelet Autoantibodies

Serum samples from patients with confirmed human granulocytic ehrlichiosis (HGE) were tested for cytoplasmic, nuclear, and platelet autoantibodies and rheumatoid factor. The indirect fluorescence antinuclear antibody test on Hep-2 cells demonstrated antinuclear titers of ≥40 and ≥160 in 44 and 10%, respectively, of serum samples from HGE patients. Two patients (4%) had anticytoplasmic (mitochondrial and spindle apparatus) antibodies with a titer of 80 and two patients (4%) had anticytoplasmic (mitochondrial) antibodies with a titer of 160 or greater. Flow cytometry was used to demonstrate antiplatelet antibodies in 80% of first serum samples from HGE patients. Rheumatoid factor was not detected. Nuclear and cytoplasmic autoantibodies are a major cause of interference when the indirect fluorescence antibody test is used to detect fluorescence of morulae inEhrlichia-infected equine neutrophils or HL-60 promyelocytes. Antiplatelet antibodies may contribute to the profound thrombocytopenia which is a characteristic laboratory feature during the acute phase of HGE infection. Whether autoantibodies precede infection or are caused by immune activation of HGE deserves further study.

scholarly journals Serological Survey of Neospora caninum and Toxoplasma gondii Co-Infection in Rodents in Northwestern Iran

2020 ◽  
Author(s):  
Naser NAZARI ◽  
Saeedeh SHOJAEE ◽  
Mahboobeh SALIMI ◽  
Mehdi MOHEBALI ◽  
Navid AHMADIFARD ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Serological Survey ◽  
Indirect Fluorescence Antibody Test ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Geographical Regions ◽  
Northwestern Iran ◽  
Indirect Fluorescence Antibody

Background: Our knowledge of the epidemiology of rodents’ parasitic agents in Iran is scarce, although some of these pathogens play an important role in human and veterinary medicine, such as Toxoplasma gondii and Neospora caninum. The purpose of this study was to determine the seroprevalence of Toxoplasma gondii and Neospora caninum in rodents of northwestern Iran between Mar and Dec 2015. Methods: Overall, 157 serum samples from rodents (101 Meriones persicus, 41 Mus musculus, and 15 Cricetulus migratorius) were assayed by the indirect fluorescence antibody test (IFAT) for antibodies to T. gondii and N. caninum Results: We found a prevalence of 20.38% (32/157) for N. caninum, 35% (55/157) for T. gondii. Co-presence of antibodies to N. caninum and T. gondii was found in 10 (6.36%) rodents. A significant association was found between the rodents species and seropositivity to N. caninum (P<0.05) but there was no association with rodents species for T. gondii. The overall prevalence of the aforementioned parasites was higher in male versus female rodents. Conclusion: The high seroprevalence of toxoplasmosis and neosporosis in rodents in the study area has implications for translocation of these infections across wider geographical regions since these rodents are mostly preyed on by cats or dogs; hence, which can transfer the parasite to other hosts.

scholarly journals Prevalence of anti-Neospora caninum antibodies in cattle from the state of Mato Grosso do Sul, Brazil

2007 ◽  
Vol 16 (3) ◽  
pp. 133-138 ◽  
Author(s):  
Leandra M. Oshiro ◽  
Maria de Fatima C. Matos ◽  
Jacqueline M. de Oliveira ◽  
Letícia A.R.C. Monteiro ◽  
Renato Andreotti
Keyword(s):  
Neospora Caninum ◽  
Antibody Test ◽  
Indirect Fluorescence Antibody Test ◽  
Sample Collection ◽  
Mato Grosso ◽  
Obligate Intracellular ◽  
Wild Canids ◽  
Bovine Neosporosis ◽  
Mato Grosso Do Sul ◽  
Indirect Fluorescence Antibody

Neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, as well as ruminants and equines. It was described in 1988 and has been known as a major cause of abortion in bovines and neuromuscular alterations and death in dogs. To estimate the prevalence of bovine neosporosis in the 22 municipalities of the so-called Estrato 1 subregion of the Brazilian state of Mato Grosso do Sul, blood samples were collected from cows aged 24 months and older, from December 2003 to March 2004. During sample collection, a questionnaire was used to gather data of epidemiological interest. The samples were subjected to serological diagnosis (indirect fluorescence antibody test - IFAT). Prevalences of 14.9% (449/2488) and 69.8% (143/205) were found for the animals and herds sampled, respectively. The variable found to be associated with seropositivity to N. caninum was abortion (OR 2.52; CI 1.25-5.06). The results revealed the presence of infection by N. caninum in the herds investigated, drawing attention to its role as a potential cause of abortion in cattle in Mato Grosso do Sul.

scholarly journals Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

2009 ◽  
Vol 29 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Leslie Scarpelli ◽  
Welber Daniel Zanetti Lopes ◽  
Matheus Migani ◽  
Katia Denise Saraiva Bresciani ◽  
Alvimar José da Costa
Keyword(s):  
Toxoplasma Gondii ◽  
Lymph Nodes ◽  
Bos Taurus ◽  
Sexual Transmission ◽  
Bos Indicus ◽  
Antibody Test ◽  
Indirect Fluorescence Antibody Test ◽  
Skeletal Musculature ◽  
Bovine Species ◽  
Indirect Fluorescence Antibody

Eighteen young steers were inoculated with Toxoplasma gondii and randomly distributed into three groups of six animals each: GI, 2.5x10(5) "P" strain oocysts, GII, 5.0x10(6) "RH" strain tachyzoites, and GIII (Control). Clinical, serological and parasitemia exams were realized. Parasite investigation by bioassay and PCR was realized on semen and fragments of skeletal musculature, lymph nodes, brain, retina, spleen, liver, lung, testicle, epididymis and seminal vesicle. Blood and semen samples were collected on days -2, -1, 1, 3, 5, 7, 14 and weekly thereafter, up to postinfection day (PID) 84. The inoculated steers (GI and GII) presented hyperthermia from PID 3 to 16. Antibodies against T. gondii were detected through the indirect fluorescence antibody test (IFAT) on PID 5 (1:16) in both inoculated groups (oocysts and tachyzoites), reaching peaks of 1:4096 on PID 7. Parasitemia outbursts occurred in all infected bovines, principally from PID 7 to 28, independent of the strain and inoculate used. Bioassays revealed the presence of parasites in semen samples of animals infected with oocysts (GI) and tachyzoites (GII) on several experimental days between PID 7 and 84. Tissue parasitism by T. gondii was diagnosed by bioassay and the PCR technique in several organ and tissue fragments. These findings suggest the possibility of sexual transmission of T. gondii in the bovine species.

scholarly journals Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis

2014 ◽  
Vol 23 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Diogo Tiago da Silva ◽  
Wilma Aparecida Starke-Buzetti ◽  
Maria Fernanda Alves-Martin ◽  
Mirian dos Santos Paixão ◽  
Michely da Silva Tenório ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Diagnostic Test ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Soluble Extract ◽  
Antigenic Protein ◽  
Complementary Method ◽  
Test Elisa ◽  
Indirect Fluorescence Antibody ◽  
Good Agreement

The aim of the present study was to evaluate the serological methods using ELISA with recombinant-rK39 (ELISA-rK-39) and soluble extract-SE (ELISA-SE) antigens, the indirect fluorescence antibody test (IFAT) in comparison to an immunochromatography rapid diagnostic test (RDT-rK39) and with a direct parasitological exam (PA) for Canine Visceral Leishmaniasis (CVL) diagnosis. The results showed that 89% (60/67) of the dogs were positive for at least one serological diagnostic test. ELISA-SE was the test that detected anti-Leishmania antibodies in the serum of the highest number of dogs (71.6%) followed by ELISA-rK39 (65.7%), IFAT (65.7%) and RDT-rK39 (55.2%). PA detected the lowest numbers (40.3%) of positive dogs. In relation to the total of examined dogs, the Kappa indexes (p ≤ 0.05) showed a good agreement between ELISA-SE and IFAT (88.1%; k = 0.7237), and it was also observed in the comparison of RDT-rK39 with ELISA-SE (83.6%, k= 0.6561), IFAT (83.5%, k= 0.6605) and PA (85.0%, k= 0.7074). A bad agreement was detected in any association of ELISA-rk39 with the other tests in either symptomatic or asymptomatic animals. ELISA as well as RDT using recombinant antigenic protein (rK39) were the methods that detected the lowest prevalence rates (33.3%) of CVL in asymptomatic dogs. In conclusion, only one test does not adequately identify dogs with CVL and it is necessary the association of two or more diagnostic tests. Because of the good agreement indexes of RDT-rK39 when evaluated with ELISA-SE, IFAT and PA it was suggested as a complementary method to be used in association with either ELISA-SE or IFAT, particularly in the symptomatic dogs. Furthermore, new studies are recommended in order to improve the sensitivity of tests mainly for asymptomatic dogs.

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