Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering

In the present work a simple and quick technique for cell seeding into tubular-shaped scaffolds, which allows a homogeneous cell distribution, was tested. The poly-L-lactide (PLLA) scaffolds, prepared via diffusion induced phase separation (DIPS), were filled with fibrin gel in order to obtain a hybrid scaffold for Vascular Tissue Engineering applications. The formation of immobilized fibrin networks on the inner surface of the tubular scaffolds was observed using confocal microscopy and SEM. Morphological analysis of the so-obtained scaffold revealed that the fibrin gel is uniformly distributed on the internal surface of the scaffold, leading to an organized structure. Moreover a penetration of the gel into the porous wall of the scaffold was observed. The in vitro endothelial cell cultures carried out in the scaffolds highlighted a faster cell proliferation inside the hybrid scaffold with respect to simple PLLA scaffold. Results show that the fibrin/PLLA hybrid scaffold may be favourably used for Vascular Tissue Engineering applications.

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Investigation of a small-diameter decellularised artery as a potential scaffold for vascular tissue engineering; biomechanical evaluation and preliminary cell seeding

Journal of the Mechanical Behavior of Biomedical Materials ◽

10.1016/j.jmbbm.2012.06.001 ◽

2012 ◽

Vol 14 ◽

pp. 130-142 ◽

Cited By ~ 22

Author(s):

E.M. Campbell ◽

P.A. Cahill ◽

C. Lally

Keyword(s):

Tissue Engineering ◽

Vascular Tissue ◽

Small Diameter ◽

Vascular Tissue Engineering ◽

Cell Seeding ◽

Biomechanical Evaluation

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Vascular tissue engineering of small-diameter blood vessels: reviewing the electrospinning approach

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.1697 ◽

2013 ◽

Vol 9 (8) ◽

pp. 861-888 ◽

Cited By ~ 75

Author(s):

Enrico Ercolani ◽

Costantino Del Gaudio ◽

Alessandra Bianco

Keyword(s):

Tissue Engineering ◽

Blood Vessels ◽

Vascular Tissue ◽

Small Diameter ◽

Vascular Tissue Engineering

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Prevascularized Micro-/Nano-Sized Spheroid/Bead Aggregates for Vascular Tissue Engineering

Nano-Micro Letters ◽

10.1007/s40820-021-00697-1 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Maedeh Rahimnejad ◽

Narges Nasrollahi Boroujeni ◽

Sepideh Jahangiri ◽

Navid Rabiee ◽

Mohammad Rabiee ◽

...

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Cardiovascular System ◽

Blood Vessels ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

Specific Cell ◽

Microvascular Network ◽

Cell Components ◽

Microvascular Engineering

AbstractEfficient strategies to promote microvascularization in vascular tissue engineering, a central priority in regenerative medicine, are still scarce; nano- and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering. Capillaries are the smallest type and in numerous blood vessels, which are distributed densely in cardiovascular system. To mimic this microvascular network, specific cell components and proangiogenic factors are required. Herein, advanced biofabrication methods in microvascular engineering, including extrusion-based and droplet-based bioprinting, Kenzan, and biogripper approaches, are deliberated with emphasis on the newest works in prevascular nano- and micro-sized aggregates and microspheres/microbeads.

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Development of a Cell-Seeding Technique Using Electric Field Force Onto Polycaprolactone Scaffolds for Vascular Tissue Engineering

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2015.1362 ◽

2015 ◽

Vol 5 (8) ◽

pp. 647-654

Author(s):

Hanfei Tang ◽

Tong Yu ◽

Kwang-Hsiao Sun ◽

Wenwen Zhang ◽

Yepeng Zhang ◽

...

Keyword(s):

Tissue Engineering ◽

Electric Field ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

Cell Seeding ◽

Field Force ◽

A Cell ◽

Electric Field Force

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In vitro cytocompatibility evaluation of collagen based scaffolds using human endothelial progenitor cells for vascular tissue engineering

Biomaterials and Tissue Engineering Bulletin ◽

10.33263/bteb114.010016 ◽

2014 ◽

Vol 1 (1-4) ◽

pp. 10-16 ◽

Cited By ~ 1

Keyword(s):

Tissue Engineering ◽

Blood Vessels ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Vascular Tissue ◽

Vascular Tissue Engineering ◽

Human Umbilical Cord ◽

Collagen Scaffolds ◽

Endothelial Progenitor

Vascular tissue engineering attempts to grow blood vessels through the use of different scaffolds that allows vascular cells such as endothelial cells to form networks and organized in vascular tissue. Various biomaterials are used to produce scaffolds that allow growth and differentiation of stem cells; depending on the cell type and applications some materials are more suitable than other. The aim of this study was to evaluate the cytocompatibility of collagen based scaffolds and to assess the capacity of endothelial progenitor cells (EPC) isolated from human umbilical cord to form vascular networks on these scaffolds. Our results show that after 5 days in culture with collagen scaffolds, the EPC remained viable, a sign of biocompatibility with the 3D scaffolds. Scanning electron microscopy showed that in the collagen scaffolds EPC organize within networks and presents an abundant extracellular matrix that strengthen the links between them. When EPC were cultured on collagenchitosan scaffolds, they are more adherent to the scaffolds compared with collagen, exibiting a good capacity to form networks. This study shows that the collagen and collagen-chitosan scaffolds are not cytotoxic for EPC and they provide the possibility of being used in vascular tissue engineering to help creating blood vessels.

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