scholarly journals Effects of Increased 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Activity in Bradyrhizobium sp. SUTN9-2 on Mung Bean Symbiosis under Water Deficit Conditions

2020 ◽  
Vol 35 (3) ◽  
pp. n/a ◽  
Author(s):  
Sukanlaya Sarapat ◽  
Pongpan Songwattana ◽  
Aphakorn Longtonglang ◽  
Kamolchanok Umnajkitikorn ◽  
Teerayoot Girdthai ◽  
...  
Keyword(s):  
Water Deficit ◽  
Mung Bean ◽  
Acc Deaminase

scholarly journals Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

2021 ◽  
Vol 8 ◽  
Author(s):  
Twinkle Chaudhary ◽  
Rajesh Gera ◽  
Pratyoosh Shukla
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Endophytic Bacterium ◽  
Fresh Weight ◽  
Root Endophyte ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Plant growth-promoting rhizobacteria (PGPR) are root endophytic bacteria used for growth promotion, and they have broader applications in enhancing specific crop yield as a whole. In the present study, we have explored the potential of Rhizobium pusense MB-17a as an endophytic bacterium isolated from the roots of the mung bean (Vigna radiata) plant. Furthermore, this bacterium was sequenced and assembled to reveal its genomic potential associated with plant growth-promoting traits. Interestingly, the root endophyte R. pusense MB-17a showed all essential PGPR traits which were determined by biochemical and PGPR tests. It was noted that this root endophytic bacterium significantly produced siderophores, indole acetic acid (IAA), ammonia, and ACC deaminase and efficiently solubilized phosphate. The maximum IAA and ammonia produced were observed to be 110.5 and 81 μg/ml, respectively. Moreover, the PGPR potential of this endophytic bacterium was also confirmed by a pot experiment for mung bean (V. radiata), whose results show a substantial increase in the plant's fresh weight by 76.1% and dry weight by 76.5% on the 60th day after inoculation of R. pusense MB-17a. Also, there is a significant enhancement in the nodule number by 66.1% and nodule fresh weight by 162% at 45th day after inoculation with 100% field capacity after the inoculation of R. pusense MB-17a. Besides this, the functional genomic annotation of R. pusense MB-17a determined the presence of different proteins and transporters that are responsible for its stress tolerance and its plant growth-promoting properties. It was concluded that the unique presence of genes like rpoH, otsAB, and clpB enhances the symbiosis process during adverse conditions in this endophyte. Through Rapid Annotation using Subsystem Technology (RAST) analysis, the key genes involved in the production of siderophores, volatile compounds, indoles, nitrogenases, and amino acids were also predicted. In conclusion, the strain described in this study gives a novel idea of using such type of endophytes for improving plant growth-promoting traits under different stress conditions for sustainable agriculture.

scholarly journals Application of rice endophytic Bradyrhizobium strain SUTN9-2 containing modified ACC deaminase to rice cultivation under water deficit conditions

2020 ◽  
Vol 15 (1) ◽  
pp. 322-334
Author(s):  
Sukanlaya Sarapat ◽  
Aphakorn Longtonglang ◽  
Kamolchanok Umnajkitikorn ◽  
Teerayoot Girdthai ◽  
Nantakorn Boonkerd ◽  
...  
Keyword(s):  
Water Deficit ◽  
Acc Deaminase ◽  
Rice Cultivation ◽  
Bradyrhizobium Strain

scholarly journals Ameliorating effects of exogenous paclobutrazol and putrescine on mung bean [Vigna radiata (L.) Wilczek] under water deficit stress

2021 ◽  
Vol 67 (No. 1) ◽  
pp. 40-45
Author(s):  
Esmaeil Babarashi ◽  
Asad Rokhzadi ◽  
Babak Pasari ◽  
Khosro Mohammadi
Keyword(s):  
Water Deficit ◽  
Seed Yield ◽  
Mung Bean ◽  
Foliar Application ◽  
Water Deficit Stress ◽  
Regression Equations ◽  
Leaf Chlorophyll ◽  
Bean Plants ◽  
Bean Yield ◽  
Different Doses

Plant growth regulators play crucial roles in modulating plant response to environmental stresses. In this experiment, the effect of different doses of paclobutrazol (PBZ) and putrescine (Put), i.e., 0, 50, 100 and 150 mg/L on mung bean in two conditions of water deficit (WD) and well-watered (WW) was investigated. The seed yield decreased due to water deficit stress, while the PBZ and Put application alleviated the damage of drought stress through increasing proline and leaf chlorophyll content and improving membrane stability, and thus increased plant yield compared to untreated control plants. According to regression equations, the high PBZ levels (150 mg/L or more) and moderate levels of Put (about 90 mg/L) were determined as the optimal concentrations to maximise mung bean yield in WD conditions. In WW conditions, the mung bean responses to PBZ were inconsistent, whereas Put application positively affected some physiological traits and seed yield. In conclusion, the physiological attributes and, subsequently, the seed yield of drought-stressed mung bean plants could be improved by foliar application of PBZ and Put.  

scholarly journals Potential of Rice Stubble as a Reservoir of Bradyrhizobial Inoculum in Rice-Legume Crop Rotation

2017 ◽  
Vol 83 (22) ◽  
Author(s):  
Pongdet Piromyou ◽  
Teerana Greetatorn ◽  
Kamonluck Teamtisong ◽  
Panlada Tittabutr ◽  
Nantakorn Boonkerd ◽  
...  
Keyword(s):  
Crop Rotation ◽  
Mung Bean ◽  
Acc Deaminase ◽  
Leguminous Plant ◽  
Content Type ◽  
Rice Growth ◽  
Legume Crop ◽  
Rotation System ◽  
Crop Rotation System ◽  
Rice Tissues

ABSTRACT Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with leguminous and nonleguminous plants, such as rice. Therefore, it can be expected that rice endophytic bradyrhizobia can be applied in the rice-legume crop rotation system. Some endophytic bradyrhizobial strains were isolated from rice (Oryza sativa L.) tissues. The rice biomass could be enhanced when supplementing bradyrhizobial strain inoculation with KNO3, NH4NO3, or urea, especially in Bradyrhizobium sp. strain SUTN9-2. In contrast, the strains which suppressed rice growth were photosynthetic bradyrhizobia and were found to produce nitric oxide (NO) in the rice root. The expression of genes involved in NO production was conducted using a quantitative reverse transcription-PCR (qRT-PCR) technique. The nirK gene expression level in Bradyrhizobium sp. strain SUT-PR48 with nitrate was higher than that of the norB gene. In contrast, the inoculation of SUTN9-2 resulted in a lower expression of the nirK gene than that of the norB gene. These results suggest that SUT-PR48 may accumulate NO more than SUTN9-2 does. Furthermore, the nifH expression of SUTN9-2 was induced in treatment without nitrogen supplementation in an endophytic association with rice. The indole-3-acetic acid (IAA) and 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase produced in planta by SUTN9-2 were also detected. Enumeration of rice endophytic bradyrhizobia from rice tissues revealed that SUTN9-2 persisted in rice tissues until rice-harvesting season. The mung bean (Vigna radiata) can be nodulated after rice stubbles were decomposed. Therefore, it is possible that rice stubbles can be used as an inoculum in the rice-legume crop rotation system under both low- and high-organic-matter soil conditions. IMPORTANCE This study shows that some rice endophytic bradyrhizobia could produce IAA and ACC deaminase and have a nitrogen fixation ability during symbiosis inside rice tissues. These characteristics may play an important role in rice growth promotion by endophytic bradyrhizobia. However, the NO-producing strains should be of concern due to a possible deleterious effect of NO on rice growth. In addition, this study reports the application of endophytic bradyrhizobia in rice stubbles, and the rice stubbles were used directly as an inoculum for a leguminous plant (mung bean). The degradation of rice stubbles leads to an increased number of SUTN9-2 in the soil and may result in increased mung bean nodulation. Therefore, the persistence of endophytic bradyrhizobia in rice tissues can be developed to use rice stubbles as an inoculum for mung bean in a rice-legume crop rotation system.

Effects of Arbuscular Mycorrhizal Fungi on Seed and Protein Yield under Water-Deficit Stress in Mung Bean

2013 ◽  
Vol 105 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Yagoob Habibzadeh ◽  
Alireza Pirzad ◽  
Mohammad Reza Zardashti ◽  
Jalal Jalilian ◽  
Omid Eini
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Water Deficit ◽  
Mycorrhizal Fungi ◽  
Mung Bean ◽  
Arbuscular Mycorrhizal ◽  
Protein Yield ◽  
Water Deficit Stress

Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant-growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase

2011 ◽  
Vol 57 (7) ◽  
pp. 578-589 ◽  
Author(s):  
Maqshoof Ahmad ◽  
Zahir A. Zahir ◽  
H. Naeem Asghar ◽  
M. Asghar
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Mung Bean ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Saline Stress ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Axenic Conditions

Twenty-five strains of plant-growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and 10 strains of rhizobia were isolated from rhizosphere soil samples and nodules of mung bean. They were screened in separate trials under salt-stressed axenic conditions. The three most effective strains of PGPR (Mk1, Pseudomonas syringae ; Mk20, Pseudomonas fluorescens ; and Mk25, Pseudomonas fluorescens biotype G) and Rhizobium phaseoli strains M1, M6, and M9 were evaluated in coinoculation for their growth-promoting activity at three salinity levels (original, 4 dS·m–1, and 6 dS·m–1) under axenic conditions. The results showed that salinity stress significantly reduced plant growth but inoculation with PGPR containing ACC deaminase and rhizobia enhanced plant growth, thus reducing the inhibitory effect of salinity. However, their combined application was more effective under saline conditions, and the combination Mk20 × M6 was the most efficient for improving seedling growth and nodulation. The effect of high ethylene concentrations on plant growth and the performance of these strains for reducing the negative impact of saline stress was also evaluated by conducting a classical triple-response bioassay. The intensity of the classical triple response decreased owing to inoculation with these strains, with the root and shoot lengths of inoculated mung bean seedlings increasing and stem diameter decreasing, which is a typical response to the dilution in a classical triple response bioassay. Thus, coinoculation with PGPR containing ACC deaminase and Rhizobium spp. could be a useful approach for inducing salt tolerance and thus improving growth and nodulation in mung bean under salt-affected conditions.

Rhizobial ACC deaminase contributes to efficient symbiosis with pea (Pisum sativum L.) under single and combined cadmium and water deficit stress

2019 ◽  
Vol 167 ◽  
pp. 103859 ◽  
Author(s):  
Andrey A. Belimov ◽  
Nadezhda Y. Zinovkina ◽  
Vera I. Safronova ◽  
Vladimir A. Litvinsky ◽  
Vladimir V. Nosikov ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Water Deficit ◽  
Acc Deaminase ◽  
Water Deficit Stress ◽  
Pisum Sativum L
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