Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant-growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase

2011 ◽  
Vol 57 (7) ◽  
pp. 578-589 ◽  
Author(s):  
Maqshoof Ahmad ◽  
Zahir A. Zahir ◽  
H. Naeem Asghar ◽  
M. Asghar
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Mung Bean ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Saline Stress ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Axenic Conditions

Twenty-five strains of plant-growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and 10 strains of rhizobia were isolated from rhizosphere soil samples and nodules of mung bean. They were screened in separate trials under salt-stressed axenic conditions. The three most effective strains of PGPR (Mk1, Pseudomonas syringae ; Mk20, Pseudomonas fluorescens ; and Mk25, Pseudomonas fluorescens biotype G) and Rhizobium phaseoli strains M1, M6, and M9 were evaluated in coinoculation for their growth-promoting activity at three salinity levels (original, 4 dS·m–1, and 6 dS·m–1) under axenic conditions. The results showed that salinity stress significantly reduced plant growth but inoculation with PGPR containing ACC deaminase and rhizobia enhanced plant growth, thus reducing the inhibitory effect of salinity. However, their combined application was more effective under saline conditions, and the combination Mk20 × M6 was the most efficient for improving seedling growth and nodulation. The effect of high ethylene concentrations on plant growth and the performance of these strains for reducing the negative impact of saline stress was also evaluated by conducting a classical triple-response bioassay. The intensity of the classical triple response decreased owing to inoculation with these strains, with the root and shoot lengths of inoculated mung bean seedlings increasing and stem diameter decreasing, which is a typical response to the dilution in a classical triple response bioassay. Thus, coinoculation with PGPR containing ACC deaminase and Rhizobium spp. could be a useful approach for inducing salt tolerance and thus improving growth and nodulation in mung bean under salt-affected conditions.

Rhizobacteria containing ACC-deaminase confer salt tolerance in maize grown on salt-affected fields

2009 ◽  
Vol 55 (11) ◽  
pp. 1302-1309 ◽  
Author(s):  
Sajid Mahmood Nadeem ◽  
Zahir Ahmad Zahir ◽  
Muhammad Naveed ◽  
Muhammad Arshad
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Ethylene Production ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt stress is one of the major constraints hampering agricultural production owing to its impact on ethylene production and nutritional imbalance. A check on the accelerated ethylene production in plants could be helpful in minimizing the negative effect of salt stress on plant growth and development. Four Pseudomonas , 1 Flavobacterium , and 1 Enterobacter strain of plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC)-deaminase were selected and their effects on growth and yield of maize were investigated to improve the salt tolerance of maize grown on salt-affected fields. The selected rhizobacterial isolates reduced or eliminated the classical “triple” response, indicating their ability to reduce stress-induced ethylene levels. Results showed that rhizobacterial strains, particularly Pseudomonas and Enterobacter spp., significantly promoted the growth and yield of maize compared with the non-inoculated control. Pseudomonas fluorescens increased plant height, biomass, cob yield, grain yield, 1000 grain mass, and straw yield of maize up to 29%, 127%, 67%, 60%, 17%, and 166%, respectively, over the control. Under stress conditions, more N, P, and K uptake and high K+–Na+ ratios were recorded in inoculated plants compared with the control. The results imply that inoculation with plant growth promoting rhizobacteria containing ACC-deaminase could be a useful approach for improving growth and yield of maize under salt-stressed conditions.

scholarly journals Salinity Stress: Toward Sustainable Plant Strategies and Using Plant Growth-Promoting Rhizobacteria Encapsulation for Reducing It

2021 ◽  
Vol 13 (22) ◽  
pp. 12758
Author(s):  
Roohallah Saberi Riseh ◽  
Marzieh Ebrahimi-Zarandi ◽  
Elahe Tamanadar ◽  
Mojde Moradi Pour ◽  
Vijay Kumar Thakur
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Ion Homeostasis ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Crop Productivity ◽  
Nutrient Mobilization ◽  
Hormonal Stimulation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salinity is one of the most important abiotic stresses that influences plant growth and productivity worldwide. Salinity affects plant growth by ionic toxicity, osmotic stress, hormonal imbalance, nutrient mobilization reduction, and reactive oxygen species (ROS). To survive in saline soils, plants have developed various physiological and biochemical strategies such as ion exchange, activation of antioxidant enzymes, and hormonal stimulation. In addition to plant adaption mechanisms, plant growth-promoting rhizobacteria (PGPR) can enhance salt tolerance in plants via ion homeostasis, production of antioxidants, ACC deaminase, phytohormones, extracellular polymeric substance (EPS), volatile organic compounds, accumulation of osmolytes, activation of plant antioxidative enzymes, and improvement of nutrients uptake. One of the important issues in microbial biotechnology is establishing a link between the beneficial strains screened in the laboratory with industry and the consumer. Therefore, in the development of biocontrol agents, it is necessary to study the optimization of conditions for mass reproduction and the selection of a suitable carrier for their final formulation. Toward sustainable agriculture, the use of appropriate formulations of bacterial agents as high-performance biofertilizers, including microbial biocapsules, is necessary to improve salt tolerance and crop productivity.

scholarly journals Plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola

2017 ◽  
Vol 9 (1) ◽  
pp. 121-128
Author(s):  
S. Kumar ◽  
M. Singh ◽  
Sushil Sharma
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Jatropha Curcas ◽  
Root Rot ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Total Phenols ◽  
Phenyl Propanoid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.

Salt-tolerant and plant-growth-promoting bacteria isolated from high-yield paddy soil

2018 ◽  
Vol 64 (12) ◽  
pp. 968-978 ◽  
Author(s):  
Shiying Zhang ◽  
Cong Fan ◽  
Yongxia Wang ◽  
Yunsheng Xia ◽  
Wei Xiao ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Paddy Soil ◽  
Effective Strain ◽  
High Yield ◽  
Saline Stress ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Plant Growth Promoting ◽  
Growth Promoting

Growth and productivity of rice is negatively affected by soil salinity. However, some salt-tolerant bacteria improve the health of plants under saline stress. In this study, 305 bacteria were isolated from paddy soil in Taoyuan, China. Among these, 162 strains were tested for salt-tolerance; 67.3%, 28.4%, and 9.3% of the strains could grow in media with NaCl concentrations of 50, 100, and 150 g/L, respectively. The phylogenic analysis of 74 of these 162 strains indicates that these bacteria belong to Bacillales (72%), Actinomycetales (22%), Rhizobiales (1%), and Oceanospirillales (4%). Among 162 strains, 30 salt-tolerant strains were screened for their plant-growth-promoting activities under axenic conditions at 3, 6, 9, and 12 g/L NaCl; 43%–97% of the strains could improve rice germination energy or germination capacity, while 63%–87% of the strains could increase shoot and root lengths. Among various plant-growth-promoting bacteria, TY0307 was the most effective strain for promoting the growth of rice, even at high salt stress. Its promotor effects were associated with its production of 1-aminocyclopropane-1-carboxycarboxylate deaminase, indole acetic acid, and siderophores; induction of proline accumulation; and reduction of the salt-induced malondialdehyde content. These results suggest that several strains isolated from paddy soil could improve rice salt tolerance and may be used in the development of biofertilizer.

scholarly journals Characterization and optimization of 1-Aminocyclopropane-1-Carboxylate Deaminase (ACCD) activity in different rhizospheric PGPR along with Microbacterium sp. strain ECI-12A

2013 ◽  
Vol 1 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Umesh P. Shrivastava ◽  
Ashok Kumar
Keyword(s):  
Plant Growth ◽  
Pcr Amplification ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Optimum Activity ◽  
Rice Rhizosphere ◽  
Acds Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Culture Growth

A total of nine strains of plant growth promoting rhizobacteria were analyzed for ACC deaminase activity, where highest ACC deaminase activity was found in Klebsiella sp strain ECI-10A (539.1 nmol α-keto butyrate/ mg protein/ h) and lowest in Microbacterium sp strain ECI-12A (122.0 nmol α-keto butyrate/ mg protein/ h). Although Microbacterium sp strain ECI-12A showed lowest level of ACC deaminase activity, but, the species of Microbacterium isolated from rhizosphere is the first report. Microbacterium sp strain ECI-12A was also analyzed under varying conditions of time, amount of 1-Aminocyclopropane-1- carboxylate (ACC), and temperature for optimization of the ACC deaminase activity. The optimum activity was recorded with the supplementation of 5mM ACC at 30°C temperature after 24h of culture growth. All the nine strains showed acdS gene in the PCR amplification of that gene. No any rhizospheric Microbacterium species showing ACC deaminase activity have been reported earlier, therefore, we report here ACC deaminase activity in Microbacterium sp ECI-12A isolated from rice rhizosphere is a novel finding. DOI: http://dx.doi.org/10.3126/ijasbt.v1i1.7921 Int J Appl Sci Biotechnol, 2013, Vol. 1(1): 11-15

Involvement ofgacSandrpoSin enhancement of the plant growth-promoting capabilities ofEnterobacter cloacaeCAL2 and UW4

2001 ◽  
Vol 47 (8) ◽  
pp. 698-705 ◽  
Author(s):  
Saleema S Saleh ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Indoleacetic Acid ◽  
Acc Deaminase ◽  
Enterobacter Cloacae ◽  
Lag Phase ◽  
Plant Growth Promoting Bacteria ◽  
Multicopy Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell density than the nontransformed strains. Transformation resulted in cells that were significantly shorter than the nontransformed cells. The transformed strains also produced significantly more IAA than the nontransformed strains. Introduction of rpoS or gacS from Pseudomonas fluorescens was associated with a reduction in the production of both antibiotics, 2,4-diacetylphloroglucinol and mono-acetylphloroglucinol, produced by Enterobacter cloacae CAL2. With Enterobacter cloacae CAL2, plasmid-borne rpoS, but not gacS, increased the level of ACC deaminase activity, while introduction of rpoS in Enterobacter cloacae UW4 caused a decrease in ACC deaminase activity. Neither gacS nor rpoS significantly affected the level of siderophores synthesized by either bacterial strain. Overproduction of either GacA or RpoS in Enterobacter cloacae CAL2 resulted in a significant increase in the root lengths of canola seedlings when seeds were treated with the bacteria, and overproduction of RpoS caused an increase in canola shoot as well as root lengths.Key words: plant growth-promoting bacteria, canola, ethylene, ACC deaminase, GacS, RpoS, indoleacetic acid, siderophores, antibiotics.

scholarly journals Genome Sequence of the Banana Plant Growth-Promoting Rhizobacterium Pseudomonas fluorescens PS006

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Rocío M. Gamez ◽  
Fernando Rodríguez ◽  
Sandra Ramírez ◽  
Yolanda Gómez ◽  
Richa Agarwala ◽  
...  
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Genome Sequence ◽  
Acc Deaminase ◽  
Whole Genome Sequence ◽  
Genome Sequences ◽  
Banana Plant ◽  
Toxic Compounds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas fluorescens is a well-known plant growth-promoting rhizobacterium (PGPR). We report here the first whole-genome sequence of PGPR P. fluorescens evaluated in Colombian banana plants. The genome sequences contains genes involved in plant growth and defense, including bacteriocins, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, and genes that provide resistance to toxic compounds.

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