Comparison of Pregnancy Outcomes Between Single-Morula Embryo Transfer and Single-Blastocyst Transfer in Fresh IVF/ICSI Cycles

Abstract Background：Previous studies have shown that in young women, single blastocyst transfer can achieve satisfactory pregnancy results, but there are few studies on the difference between fresh and frozen-thawed single blastocyst transfer. In this retrospective analysis, the purpose of this study was to compare the pregnancy outcomes of young patients who received fresh or frozen-thawed single blastocyst transfer, so as to provide further clinical strategies for single blastocyst transfer.Methods: A retrospective analysis of patients aged ≤35 years old undergoing single blastocyst transfer was performed from January 2018 to December 2018 in the reproductive center of the Second Affiliated Hospital of Wenzhou Medical University. A total of 901 patients were involved and were divided into two groups according to the type of transfer cycle, 693 of which were in the fresh embryo transfer cycle (group A) and 208 of which were in the frozen-thawed embryo transfer cycle (group B). The laboratory and pregnancy outcomes were compared between the groups. Results：The number of oocytes retrieved in group B was significantly more than in group A ( P＜0.05). The early miscarriage rate in group B was higher than in group A, with significant difference(P＜0.05). There were no significant differences in other basic conditions and obstetric pregnancy outcomes between the two groups.Conclusions：Favorable pregnancy outcomes could be obtained in both fresh and frozen-thawed single blastocyst transfer in young patients without increasing the birth defect rate. For patients with risk factors of OHSS such as more oocyte retrieval, ovarian hyper-response, or risk factors of ectopic pregnancy such tubal infertility and the history of ectopic pregnancy, frozen-thawed single blastocyst transfer could be highly recommended in clinical practice. However, since the early miscarriage rate is higher in frozen-thawed embryo transfer group, further research is needed to figure out the cause and possible solutions.

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Comparison of pregnancy outcomes in young patients following fresh versus frozen single blastocyst transfer:A retrospective study

10.21203/rs.3.rs-48026/v2 ◽

2021 ◽

Author(s):

yanhong wu ◽

Chang Liu ◽

Chaochao Gong ◽

Haitao Xi ◽

Yanghua Fu ◽

...

Keyword(s):

Embryo Transfer ◽

Pregnancy Outcomes ◽

Young Patients ◽

Blastocyst Transfer ◽

Miscarriage Rate ◽

Single Blastocyst Transfer ◽

Group A ◽

Early Miscarriage ◽

Group B ◽

Cycle Group

Abstract Background：Previous studies have shown that, in young women, single blastocyst transfer can achieve satisfactory pregnancy results; however, few studies exist regarding the difference between fresh and frozen-thawed single blastocyst transfer. To provide further clinical strategies for single blastocyst transfer, the purpose of this retrospective analysis was to compare the pregnancy outcomes of young patients who received fresh or frozen-thawed single blastocyst transfer.Methods: A retrospective analysis of patients, aged ≤35 years, who underwent single blastocyst transfer was conducted from January 2018 to December 2018 in the reproductive center of the Second Affiliated Hospital of Wenzhou Medical University (Wenzhou, China). In total, 901 patients were included and were divided into two groups, based on the type of transfer cycle: the fresh embryo transfer cycle group (group A; n=693) and the frozen-thawed embryo transfer cycle group (group B; n=208). The laboratory and pregnancy outcomes were compared between the groups.Results：The number of oocytes retrieved in group B was significantly higher than in group A (P＜0.05). The early miscarriage rate in group B was significantly higher than in group A and was significantly different (P＜0.05). Frozen-thawed single blastocyst transplantation was an independent risk factor for early miscarriage. Other basic conditions and obstetric pregnancy outcomes were not significantly different between the two groups.Conclusions：Favourable pregnancy outcomes could be obtained with fresh and frozen-thawed single blastocyst transfer in young patients. However, because the early miscarriage rate was higher in the frozen-thawed embryo transfer group, further research is needed to determine the cause and possible solutions.

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The effect of Day 3 cell number on pregnancy outcomes in vitrified-thawed single blastocyst transfer cycles

Human Reproduction ◽

10.1093/humrep/deaa209 ◽

2020 ◽

Vol 35 (11) ◽

pp. 2478-2487

Author(s):

Jiayi Wu ◽

Jie Zhang ◽

Yanping Kuang ◽

Qiuju Chen ◽

Yun Wang

Keyword(s):

Pregnancy Rate ◽

Live Birth ◽

Young Women ◽

Pregnancy Outcomes ◽

Cell Number ◽

Blastocyst Transfer ◽

Cell Group ◽

Single Blastocyst Transfer ◽

Significant Difference ◽

Blastomere Number

Abstract STUDY QUESTION Does cell number on Day 3 have an impact on pregnancy outcomes in vitrified-thawed single blastocyst transfer cycles? SUMMARY ANSWER A low Day 3 cell number (≤5 cells) was independently associated with decreased live birth rate (LBR) during single blastocyst transfer cycles in young women. WHAT IS KNOWN ALREADY Day 3 cell number is an effective predictor of IVF success rates when transferring cleavage stage embryos. However, the association between Day 3 blastomere number and pregnancy outcomes after blastocyst transfer is still unknown. STUDY DESIGN, SIZE, DURATION A retrospective cohort study of 3543 patients who underwent frozen-thawed single blastocyst transfers from January 2013 to June 2018 at a tertiary-care academic medical center. PARTICIPANTS/MATERIALS, SETTING, METHODS Patients were grouped into six groups according to the Day 3 cell number: ≤4 cells, 5 cells, 6 cells, 7 cells, 8 cells and >8 cells. The primary outcome measure was LBR. A logistic regression analysis was performed to explore the independent association between Day 3 blastomere number and LBR after adjustment for some potential confounders. MAIN RESULTS AND THE ROLE OF CHANCE In women <35 years old, the LBR varied significantly according to Day 3 cell number, with the rate of 31.2%, 34.4%, 41.9%, 45.1%, 48.1% and 48.2% for the ≤4-cell, 5-cell, 6-cell, 7-cell, 8-cell and >8-cell groups, respectively (P < 0.001). This significant difference was also observed in the high- and low-quality blastocyst subgroups of young women. However, for women ≥35 years old, the rate of live birth was similar between groups. Furthermore, after accounting for confounding factors, the LBR was significantly decreased in the ≤4-cell (adjusted odds ratio (aOR): 0.62, 95% CI: 0.48–0.80, P < 0.001) and 5-cell (aOR: 0.73, 95% CI: 0.57–0.92, P = 0.009) groups as compared to the 8-cell group. Likewise, the blastocysts arising from ≤4-cell (aOR: 0.73, 95% CI: 0.57–0.93, P = 0.010) or 5-cell (aOR: 0.77, 95% CI: 0.61–0.97, P = 0.024) embryos were associated with lower clinical pregnancy rate than those from 8-cell embryos. No significant differences were observed in biochemical pregnancy rate and miscarriage rate. LIMITATIONS, REASONS FOR CAUTION A limitation of the current study was its retrospective design. Future prospective studies are needed to confirm our findings. WIDER IMPLICATIONS OF THE FINDINGS Our observations suggested that a low Day 3 cell number was related to decreased LBR after blastocyst transfer in young women, which provided vital information for clinicians in selecting blastocyst during IVF treatment. STUDY FUNDING/COMPETING INTEREST(S) This study was supported by the National Natural Science Foundation of China (NSFC) (31770989 to Y.W.; 81671520 to Q.C.) and the Shanghai Ninth People’s Hospital Foundation of China (JYLJ030 to Y.W.). The authors have no conflicts of interest to declare. TRIAL REGISTRATION NUMBER N/A.

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Morbid obesity and pregnancy outcomes after single blastocyst transfer: a retrospective, North American study

Journal of Assisted Reproduction and Genetics ◽

10.1007/s10815-017-0883-9 ◽

2017 ◽

Vol 34 (4) ◽

pp. 451-457 ◽

Cited By ~ 8

Author(s):

Miguel Russo ◽

Senem Ates ◽

Talya Shaulov ◽

Michael H. Dahan

Keyword(s):

Morbid Obesity ◽

North American ◽

Pregnancy Outcomes ◽

Blastocyst Transfer ◽

American Study ◽

North American Study ◽

Single Blastocyst Transfer

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Evaluation of pregnancy outcome after establishment of single blastocyst transfer (SET) criteria in in vitro fertilization and egg recipient (IVF/ER) embryo transfer (ET) cycles

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.07.395 ◽

2007 ◽

Vol 88 ◽

pp. S122-S123

Author(s):

D.C. Merryman ◽

S.E. Stringfellow ◽

C.Y. Gibbs ◽

C.A. Long ◽

V.L. Houserman ◽

...

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Outcome ◽

Embryo Transfer ◽

Blastocyst Transfer ◽

Vitro Fertilization ◽

Single Blastocyst Transfer

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Evaluation of Pregnancy Outcomes of Vitrified-Warmed Blastocyst Transfer before and after Endometrial Receptivity Analysis in Identical Patients with Recurrent Implantation Failure

Fertility & Reproduction ◽

10.1142/s2661318221500043 ◽

2021 ◽

pp. 1-7

Author(s):

Yuta Kasahara ◽

Tomoko Hashimoto ◽

Ryo Yokomizo ◽

Yuya Takeshige ◽

Koki Yoshinaga ◽

...

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Pregnancy Outcomes ◽

Endometrial Receptivity ◽

Pregnancy Rates ◽

Blastocyst Transfer ◽

Implantation Failure ◽

Recurrent Implantation Failure ◽

Clinical Value ◽

Ongoing Pregnancy Rate

Background:The clinical value of personalized embryo transfer (pET) guided by the endometrial receptivity analysis (ERA) tests for recurrent implantation failure (RIF) cases is still unclear. The aim of this study is to clarify the efficacy of ERA leading to personalization of the day of embryo transfer (ET) in RIF patients. Methods: A retrospective study was performed for 94 patients with RIF who underwent ERA between July 2015 and December 2019. Pregnancy outcomes in a previous vitrified-warmed blastocyst transfer (previous VBT) and a personalized vitrified-warmed blastocyst transfer (pVBT) in identical patients were compared. The details of each pVBT were further analyzed between patients in a non-displaced group, which indicated “receptive” cases in ERA results and those who were in the displaced group, which indicated “non-receptive” cases. Results:When the pregnancy rate, both per patient and per transfer cycle, of previous VBT and pVBT were compared, a significant increase in pVBT was observed between the two methods (5.3% vs. 62.8%, 4.4% vs. 47.9%, respectively). The pregnancy rates, implantation rates, and clinical pregnancy rates of the first pVBT were significantly higher in the displaced group than the non-displaced group. The cumulative ongoing pregnancy rate of the displaced group tended to be higher compared to that of the non-displaced group in the first pVBT, although the difference was not statistically significant (51.0% vs. 31.1%, [Formula: see text] = 0.06). Conclusions:Our study demonstrates that pVBT guided by ERA tests may improve pregnancy outcomes in RIF patients whose window of implantation (WOI) is displaced, and its effect may be more pronounced at the first pVBT. The displacement of WOI may be considered to be one of the causes of RIF, and its adjustment may contribute to the improvement of pregnancy outcomes in RIF patients.

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