Key Points of High-Yield Cultivation Techniques for Greenhouse Tomato

Abstract Raspberry ketone is a widely used flavor compound in food and cosmetic industry. Several processes for its biocatalytic production have already been described, but either with the use of genetically modified organisms (GMOs) or incomplete conversion of the variety of precursors that are available in nature. Such natural precursors are rhododendrol glycosides with different proportions of (R)- and (S)-rhododendrol depending on the origin. After hydrolysis of these rhododendrol glycosides, the formed rhododendrol enantiomers have to be oxidized to obtain the final product raspberry ketone. To be able to achieve a high conversion with different starting material, we assembled an alcohol dehydrogenase toolbox that can be accessed depending on the optical purity of the intermediate rhododendrol. This is demonstrated by converting racemic rhododendrol using a combination of (R)- and (S)-selective alcohol dehydrogenases together with a universal cofactor recycling system. Furthermore, we conducted a biocatalytic cascade reaction starting from naturally derived rhododendrol glycosides by the use of a glucosidase and an alcohol dehydrogenase to produce raspberry ketone in high yield. Key points • LB-ADH, LK-ADH and LS-ADH oxidize (R)-rhododendrol • RR-ADH and ADH1E oxidize (S)-rhododendrol • Raspberry ketone production via glucosidase and alcohol dehydrogenases from a toolbox Graphical abstract

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Musculoskeletal Imaging Volume 1

10.1093/med/9780190938161.001.0001 ◽

2019 ◽

Keyword(s):

Clinical Problem ◽

High Yield ◽

Core Knowledge ◽

Congenital Diseases ◽

Anatomy And Physiology ◽

Key Points ◽

Salient Points ◽

Key Topics ◽

Structured Approach ◽

Readable Format

This volume meets the needs of radiology residents to become adept at interpreting musculoskeletal (MSK) imaging studies. It does so by presenting core knowledge and fundamentals that must be learned to accurately and effectively interpret MSK studies by the trainee and non-specialist. The goal is to impart to residents, as well as to refresh for practitioners, essential facts in a concise and readable format so the reader becomes conversant with all imaging modalities used and the essentials of interpretation and technique. Other resources are at too high a level for the resident in training or contain far more information than a resident can easily assimilate during a rotation. The book is part of the Rotations in Radiology series for residents, which defines and encapsulates core knowledge for areas within Radiology, offering a guided, structured approach to imaging diagnosis. It contains sections on 10 key topics in MSK radiology: trauma; arthritis; tumors and tumor-like conditions; metabolic, hematopoietic, endocrine, and deposition diseases; infectious diseases; arthrography; internal derangements of the joints; congenital diseases; and ultrasound. Each section begins with an overview chapter, orienting the reader to the specific concerns and issues related to imaging that anatomic region or category of problem. Each clinical problem or diagnosis is concisely covered to provide a targeted discussion and highlight salient points. For each topic, concise chunks of text will review: definition; clinical features; anatomy and physiology; how to appraoch the image; what not to miss; differential diagnosis; common variants if pertinent; clinical issues; key points; high yield references.

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Musculoskeletal Imaging Volume 2

10.1093/med/9780190938178.001.0001 ◽

2019 ◽

Keyword(s):

Clinical Problem ◽

High Yield ◽

Core Knowledge ◽

Congenital Diseases ◽

Anatomy And Physiology ◽

Key Points ◽

Salient Points ◽

Key Topics ◽

Structured Approach ◽

Readable Format

This volume meets the needs of radiology residents to become adept at interpreting musculoskeletal (MSK) imaging studies. It does so by presenting core knowledge and fundamentals that must be learned to accurately and effectively interpret MSK studies by the trainee and non-specialist. The goal is to impart to residents, as well as to refresh for practitioners, essential facts in a concise and readable format so the reader becomes conversant with all imaging modalities used and the essentials of interpretation and technique. Other resources are at too high a level for the resident in training or contain far more information than a resident can easily assimilate during a rotation. The book is part of the Rotations in Radiology series for residents, which defines and encapsulates core knowledge for areas within Radiology, offering a guided, structured approach to imaging diagnosis. It contains sections on 10 key topics in MSK radiology: trauma; arthritis; tumors and tumor-like conditions; metabolic, hematopoietic, endocrine, and deposition diseases; infectious diseases; arthrography; internal derangements of the joints; congenital diseases; and ultrasound. Each section begins with an overview chapter, orienting the reader to the specific concerns and issues related to imaging that anatomic region or category of problem. Each clinical problem or diagnosis is concisely covered to provide a targeted discussion and highlight salient points. For each topic, concise chunks of text will review: definition; clinical features; anatomy and physiology; how to appraoch the image; what not to miss; differential diagnosis; common variants if pertinent; clinical issues; key points; high yield references.

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Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

BMC Research Notes ◽

10.1186/s13104-017-2880-6 ◽

2017 ◽

Vol 10 (1) ◽

Cited By ~ 12

Author(s):

Hossein Motedayyen ◽

Nafiseh Esmaeil ◽

Nader Tajik ◽

Fahimeh Khadem ◽

Somayeh Ghotloo ◽

...

Keyword(s):

Epithelial Cells ◽

High Yield ◽

Amniotic Epithelial Cells ◽

Key Points ◽

Human Amniotic Epithelial Cells

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Proteases of Wood Rot Fungi with Emphasis on the GenusPleurotus

BioMed Research International ◽

10.1155/2015/290161 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 11

Author(s):

Fabíola Dorneles Inácio ◽

Roselene Oliveira Ferreira ◽

Caroline Aparecida Vaz de Araujo ◽

Tatiane Brugnari ◽

Rafael Castoldi ◽

...

Keyword(s):

Ligninolytic Enzymes ◽

Immune Defense ◽

Production Costs ◽

High Yield ◽

Nutrient Requirements ◽

Physiological Conditions ◽

Physiological Importance ◽

Industrial Sectors ◽

Living Organisms ◽

Cultivation Techniques

Proteases are present in all living organisms and they play an important role in physiological conditions. Cell growth and death, blood clotting, and immune defense are all examples of the importance of proteases in maintaining homeostasis. There is growing interest in proteases due to their use for industrial purposes. The search for proteases with specific characteristics is designed to reduce production costs and to find suitable properties for certain industrial sectors, as well as good producing organisms. Ninety percent of commercialized proteases are obtained from microbial sources and proteases from macromycetes have recently gained prominence in the search for new enzymes with specific characteristics. The production of proteases from saprophytic basidiomycetes has led to the identification of various classes of proteases. The genusPleurotushas been extensively studied because of its ligninolytic enzymes. The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation. There are few studies in the literature about proteases ofPleurotusspp. This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

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