scholarly journals The Effect of Capsaicin on IGF-I and IGF-IR Expression in Ovarian Granulosa Cells

2020 ◽  
Vol 71 (1) ◽  
pp. 1977
Author(s):  
S. GULER ◽  
Β. ZIK
Keyword(s):  
Growth Factor ◽  
Granulosa Cells ◽  
Ovarian Development ◽  
Ovary Development ◽  
Insulin Like Growth Factor ◽  
Sprague Dawley ◽  
Factor I ◽  
Ovarian Granulosa Cells ◽  
Igf I ◽  
Growth Factor I

Capsaicin (trans-8-methyl-N-vanillyl-6-noneadamide) is a pungent ingredient in red peppers from the Capsicum family. Insulin-like growth factor-I (IGF-I) is expressed in granulosa cells and has an important role in ovarian development. However, there are no data about the IGF-I expression in ovarian granulosa cells after capsaicin treatment. The aim of this study was to investigate the expression of IGF-I and its receptor (insulin-like growth factor-I receptor [IGF-IR]) in primary rat ovarian granulosa cells after low and high doses of capsaicin treatment. For this, granulosa cells were isolated and cultured from ovaries of 30-day-old female Sprague-Dawley rats. Granulosa cell plates were divided into four groups as cell control (C), vehicle control (V), and 50 μM and 150 μM capsaicin groups. In experimental groups, granulosa cells were exposed to capsaicin for 24 hours and immunocytochemistry was performed afterwards using anti-IGF-I and anti-IGF-IR antibodies. Both IGF-I and IGF-IR expressions were found to be significantly increased in parallel to the capsaicin doses. Elevated levels of IGF-I may be a risk factor for ovarian development. Because of the crucial role of IGF-I in ovary development, capsaicin treatment can be effective on follicular development and/or disorders characterized by high IGF-I levels.

Salivary insulin-like growth factor-I originates from local synthesis

1992 ◽  
Vol 135 (1) ◽  
pp. 85-NP ◽  
Author(s):  
J. Ryan ◽  
T. Mantle ◽  
S. McQuaid ◽  
D. C. Costigan
Keyword(s):  
Growth Factor ◽  
Gel Chromatography ◽  
Insulin Like Growth Factor ◽  
Sprague Dawley ◽  
Local Synthesis ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I ◽  
Rat Parotid ◽  
Dependent Growth

ABSTRACT Insulin-like growth factor-I (IGF-I) is a GH-dependent growth factor found in its highest concentrations in plasma. It is also measurable in saliva. The origins of salivary IGF-I concentrations were studied. Intracardial administration of Sprague–Dawley rats with 125I-labelled IGF-I and subsequent analysis of plasma and saliva samples by exclusion gel chromatography and SDS-PAGE, followed by autoradiography, demonstrated the apparent inability of IGF-I to cross from the plasma pool through to saliva. 125I-Labelled IGF-I was not chromatographed immediately before injection, resulting in administration of free iodide along with the iodinated peptide. This free iodide was demonstrable in saliva, indicating that movement of substances from plasma to saliva was measurable using the levels of 125I activity administered. Free iodide in saliva was not contributed to by 125I-labelled IGF-I degradation since 125I-labelled IGF-I was shown to be stable in saliva over 24 h. These data indicated that IGF-I in saliva is produced locally. Identification of a 4·7 kb IGF-I mRNA transcript in rat parotid salivary gland was consistent with IGF-I synthesis within that tissue. Journal of Endocrinology (1992) 135, 85–90

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