<i>Nelumbo nucifera</i> Leaf Extracts Inhibit Melanogenesis in B16 Melanoma Cells and Guinea Pigs through Downregulation of CREB/MITF Activation

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

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Cell adhesion in a dynamic flow system as compared to static system. Glycosphingolipid-glycosphingolipid interaction in the dynamic system predominates over lectin- or integrin-based mechanisms in adhesion of B16 melanoma cells to non-activated endothelial cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)41921-7 ◽

1992 ◽

Vol 267 (24) ◽

pp. 17264-17270

Author(s):

N Kojima ◽

M Shiota ◽

Y Sadahira ◽

K Handa ◽

S Hakomori

Keyword(s):

Endothelial Cells ◽

Cell Adhesion ◽

Dynamic System ◽

Flow System ◽

Melanoma Cells ◽

B16 Melanoma ◽

Static System ◽

Dynamic Flow ◽

B16 Melanoma Cells

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Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells

Natural Product Communications ◽

10.1177/1934578x211019264 ◽

2021 ◽

Vol 16 (5) ◽

pp. 1934578X2110192

Author(s):

Yuki Ohno ◽

Shiori Kondo ◽

Kiho Tajima ◽

Toshiyuki Shibata ◽

Tomohiro Itoh

Keyword(s):

Melanoma Cells ◽

B16 Melanoma ◽

Physiological Effects ◽

Related Protein ◽

Eisenia Bicyclis ◽

Melanin Production ◽

Melanocyte Stimulating Hormone ◽

B16 Melanoma Cells ◽

Anti Cancer ◽

Tyrosinase Related Protein

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

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