scholarly journals Hybrid embryo formation in an intersubgeneric cross of soybean (Glycin max MERILL) with a wild relative (G. tomentella HAYATA).

1985 ◽  
Vol 35 (4) ◽  
pp. 363-374 ◽  
Author(s):  
Takako SAKAI ◽  
Norihiko KAIZUMA
Keyword(s):  
Wild Relative ◽  
Embryo Formation ◽  
Hybrid Embryo

Embryo Rescue Culture Medium for Improved Synthesis of 4x Wheat × Rye Hybrids

1994 ◽  
Vol 42 (4) ◽  
pp. 493
Author(s):  
CH Balatero ◽  
NL Darvey
Keyword(s):  
Embryo Rescue ◽  
Favourable Effect ◽  
Embryo Formation ◽  
Step Procedure ◽  
Hybrid Embryo ◽  
One Step ◽  
Well Differentiated ◽  
Semi Solid ◽  
Optimum Recovery

The cross-incompatibility barrier between 4x wheat and rye has limited the genetic base for triticale breeding. Experiments designed to improve the synthesis of wheat-rye amphihaploids were conducted. The effects of 2,4-D on crossability and 3x hybrid embryo differentiation, and the influence of one-step and two-step media on the culture of immature 3x embryos in vitro, were investigated. Application of 10 mg L-1 2,4-D slightly improved seed set but significantly reduced the frequency of normal embryos. In contrast to the reported favourable effect of 2,4-D on haploid embryo formation in wheat × maize crosses, the application of 2,4-D in the present study offers no real advantage on amphihaploid embryo formation from 4x wheat × rye crosses. For small and immature wheat-rye hybrid (3x) embryos, optimum recovery in vitro was obtained via a two-step procedure consisting of a semi-solid MN medium followed by MS medium supplemented with IAA (1 mg L-1) and BAP (1 mg L-1). For bigger and well-differentiated embryos, the use of a one-step Gamborg's B5 medium was sufficient.

Glycine canescens F. J. Herm., a Wild Relative of the Soybean 1

Crop Science ◽  
1975 ◽  
Vol 15 (6) ◽  
pp. 879-881 ◽  
Author(s):  
C. A. Newell ◽  
T. Hymowitz
Keyword(s):  
Wild Relative

scholarly journals Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
P. O. Olsson ◽  
A. H. Tinson ◽  
N. Al Shamsi ◽  
K. S. Kuhad ◽  
R. Singh ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Nuclear Transfer ◽  
Large Scale ◽  
Donor Cell ◽  
Blastocyst Formation ◽  
Short Term ◽  
Embryo Formation ◽  
Large Expansion ◽  
Breed Differences ◽  
Breed Comparison

AbstractCloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.

scholarly journals Depressurization-Induced Nucleation in the “Polylactide-Carbon Dioxide” System: Self-Similarity of the Bubble Embryos Expansion

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1115
Author(s):  
Dmitry Zimnyakov ◽  
Marina Alonova ◽  
Ekaterina Ushakova
Keyword(s):  
Initial Rate ◽  
Initial Pressure ◽  
External Pressure ◽  
Self Similarity ◽  
Embryo Formation ◽  
Linear Behavior ◽  
Joint Influence ◽  
External Pressures ◽  
Adiabatic Cooling ◽  
Self Similar

Self-similar expansion of bubble embryos in a plasticized polymer under quasi-isothermal depressurization is examined using the experimental data on expansion rates of embryos in the CO2-plasticized d,l-polylactide and modeling the results. The CO2 initial pressure varied from 5 to 14 MPa, and the depressurization rate was 5 × 10−3 MPa/s. The constant temperature in experiments was in a range from 310 to 338 K. The initial rate of embryos expansion varied from ≈0.1 to ≈10 µm/s, with a decrease in the current external pressure. While modeling, a non-linear behavior of CO2 isotherms near the critical point was taken into account. The modeled data agree satisfactorily with the experimental results. The effect of a remarkable increase in the expansion rate at a decreasing external pressure is interpreted in terms of competing effects, including a decrease in the internal pressure, an increase in the polymer viscosity, and an increase in the embryo radius at the time of embryo formation. The vanishing probability of finding the steadily expanding embryos for external pressures around the CO2 critical pressure is interpreted in terms of a joint influence of the quasi-adiabatic cooling and high compressibility of CO2 in the embryos.

scholarly journals Fertilization Narratives in the Art of Gustav Klimt, Diego Rivera and Frida Kahlo: Repression, Domination and Eros among Cells

Leonardo ◽  
2011 ◽  
Vol 44 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Scott F. Gilbert ◽  
Sabine Brauckmann
Keyword(s):  
20Th Century ◽  
Human Embryos ◽  
Diego Rivera ◽  
Frida Kahlo ◽  
Embryo Formation ◽  
Human Fertilization ◽  
Gustav Klimt ◽  
The Universe ◽  
Early Human

Fertilization narratives are powerful biological stories that can be used for social ends, and 20th-century artists have used fertilization-based imagery to convey political and social ideas. In Danae, Gustav Klimt used an esoteric stage of early human embryos to indicate successful fertilization and the inability of government repression to stifle creativity. In Man, Controller of the Universe, Diego Rivera painted a mural of a man controlling an ovulating ovary, depicting Trotsky's view that society will rationally regulate human fertilization. His former wife, Frida Kahlo, refuted this view in Moses: Nucleus of Creation, wherein she painted images of fertilization and embryo formation as the ultimate acts of erotic consummation and generation.

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