Detection of High-Molecular Weight Mucin-like Glycoprotein-A (HMGP-A) of Human Milk by Chicken Egg Yolk Antibody

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

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Chicken Egg Yolk Antibody (IgY) Controls Solobacterium moorei Under In Vitro and In Vivo Conditions

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-012-9869-3 ◽

2012 ◽

Vol 168 (6) ◽

pp. 1448-1458 ◽

Cited By ~ 6

Author(s):

Xiaoyu Li ◽

He Liu ◽

Yongping Xu ◽

Fanxing Xu ◽

Linhui Wang ◽

...

Keyword(s):

Egg Yolk ◽

Chicken Egg ◽

Egg Yolk Antibody ◽

Chicken Egg Yolk

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Passive immunotherapy using chicken egg yolk antibody (IgY) against diarrheagenic E. coli: A systematic review and meta-analysis

International Immunopharmacology ◽

10.1016/j.intimp.2021.108381 ◽

2021 ◽

pp. 108381

Author(s):

Mukunthan Karthikeyan ◽

Srichandrasekar Thuthikkadu Indhuprakash ◽

Gayathri Gopal ◽

Senthil Visaga Ambi ◽

Uma Maheswari Krishnan ◽

...

Keyword(s):

Systematic Review ◽

Meta Analysis ◽

Egg Yolk ◽

Passive Immunotherapy ◽

E Coli ◽

Chicken Egg ◽

Egg Yolk Antibody ◽

Chicken Egg Yolk

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Specific Chicken Egg Yolk Antibody Improves the Protective Response againstGallibacterium anatisInfection

Infection and Immunity ◽

10.1128/iai.00619-18 ◽

2018 ◽

Vol 87 (3) ◽

Cited By ~ 1

Author(s):

Jiao Jiao Zhang ◽

Tae Yoon Kang ◽

Taeho Kwon ◽

Hyebin Koh ◽

Nisansala Chandimali ◽

...

Keyword(s):

Brain Heart Infusion ◽

Egg Yolk ◽

Infection Model ◽

Antibody Treatment ◽

Protective Response ◽

Content Type ◽

Chicken Egg ◽

Antibody Titers ◽

Egg Yolk Antibody ◽

Chicken Egg Yolk

ABSTRACTGallibacterium anatisis a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against aG. anatisinfection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus ofGallibacteriumtoxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth ofG. anatisin brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated inG. anatis-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera ofG. anatis-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response againstG. anatisinfection in chicks.

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