Purification and Characterization of a Novel Fungal α-Glucosidase fromMortierella alliaceawith High Starch-hydrolytic Activity

2002 ◽  
Vol 66 (11) ◽  
pp. 2415-2423 ◽  
Author(s):  
Yoshio TANAKA ◽  
Tsunehiro AKI ◽  
Yûki HIDAKA ◽  
Yûji FURUYA ◽  
Seiji KAWAMOTO ◽  
...  
Keyword(s):  
Hydrolytic Activity ◽  
Purification And Characterization ◽  
High Starch

Purification and characterization of an alkaline amylopullulanase with both α-1,4 and α-1,6 hydrolytic activity from alkalophilic Bacillus sp. KSM-1378

1995 ◽  
Vol 1243 (3) ◽  
pp. 315-324 ◽  
Author(s):  
Katsutoshi Ara ◽  
Katsuhisa Saeki ◽  
Kazuaki Igarashi ◽  
Mikio Takaiwa ◽  
Takaaki Uemura ◽  
...  
Keyword(s):  
Hydrolytic Activity ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Alkalophilic Bacillus

Cloning, expression, purification and characterization of HSP105

2010 ◽  
Vol 34 (8) ◽  
pp. S46-S46
Author(s):  
Dong Han ◽  
Huang Xu
Keyword(s):  
Purification And Characterization

Cloning and high level expression of Cynodon dactylon (Bermuda grass) pollen profilin (Cyn d 12) in Escherichia coli: purification and characterization of the allergen

1997 ◽  
Vol 27 (11) ◽  
pp. 1307-1313 ◽  
Author(s):  
J. A. ASTURIAS ◽  
M. C. ARILLA ◽  
N. GOMEZ-BAYON ◽  
J. MARTINEZ ◽  
A. MARTINEZ ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Grass Pollen ◽  
Cynodon Dactylon ◽  
Bermuda Grass ◽  
Purification And Characterization ◽  
High Level Expression ◽  
Bermuda Grass Pollen ◽  
D 12 ◽  
High Level

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization

Purification and Characterization of Tissue-Type Plasminogen Activator in Maxillary Mucosa with Chronic Inflammation

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi
Keyword(s):  
Plasminogen Activator ◽  
Chronic Inflammation ◽  
Gel Filtration ◽  
Potassium Acetate ◽  
Tissue Type ◽  
Purification And Characterization ◽  
Tissue Type Plasminogen Activator ◽  
Urokinase Inhibitor ◽  
Type Plasminogen Activator

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

Purification and Characterization of a Novel Serine Protease from the Fruiting Bodies of a Rare Edible Medicinal Mushroom, Lyophyllum shimeji (Agaricomycetes)

2016 ◽  
Vol 18 (6) ◽  
pp. 547-554 ◽  
Author(s):  
Xueran Geng ◽  
Rigen Te ◽  
Guoting Tian ◽  
Yongchang Zhao ◽  
Liyan Zhao ◽  
...  
Keyword(s):  
Serine Protease ◽  
Medicinal Mushroom ◽  
Fruiting Bodies ◽  
Purification And Characterization ◽  
Lyophyllum Shimeji

Purification and Characterization of Cellulase from Aspergillusniger Causing Soft Rot of White Yam in Three Yam-growing Environments in Nigeria

2019 ◽  
Vol 9 (6) ◽  
pp. p90133
Author(s):  
Popoola O. Omokunmi ◽  
Ajibade G Abdulsalami ◽  
M.S Abba ◽  
Daniel Adams ◽  
D. David
Keyword(s):  
Soft Rot ◽  
Purification And Characterization ◽  
White Yam
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