lyophyllum shimeji
Recently Published Documents


TOTAL DOCUMENTS

23
(FIVE YEARS 1)

H-INDEX

9
(FIVE YEARS 0)

2021 ◽  
Vol 22 (21) ◽  
pp. 11598
Author(s):  
Jia-Qi Lu ◽  
Wei-Wei Shi ◽  
Meng-Jie Xiao ◽  
Yun-Sang Tang ◽  
Yong-Tang Zheng ◽  
...  

Ribosome-inactivating proteins (RIPs) hydrolyze the N-glycosidic bond and depurinate a specific adenine residue (A-4324 in rat 28S ribosomal RNA, rRNA) in the conserved α-sarcin/ricin loop (α-SRL) of rRNA. In this study, we have purified and characterized lyophyllin, an unconventional RIP from Lyophyllum shimeji, an edible mushroom. The protein resembles peptidase M35 domain of peptidyl-Lys metalloendopeptidases. Nevertheless, protein either from the mushroom or in recombinant form possessed N-glycosidase and protein synthesis inhibitory activities. A homology model of lyophyllin was constructed. It was found that the zinc binding pocket of this protein resembles the catalytic cleft of a classical RIP, with key amino acids that interact with the adenine substrate in the appropriate positions. Mutational studies showed that E122 may play a role in stabilizing the positively charged oxocarbenium ion and H121 for protonating N-3 of adenine. The tyrosine residues Y137 and Y104 may be used for stacking the target adenine ring. This work first shows a protein in the peptidase M35 superfamily based on conserved domain search possessing N-glycosidase activity.



Author(s):  
D Xu ◽  
C Q Sun ◽  
B Li ◽  
J J Xu ◽  
H Zhao ◽  
...  


2016 ◽  
Vol 7 (1) ◽  
pp. 61-65
Author(s):  
Ertuğrul Sesli ◽  
Ayşegül Topcu Sesli
Keyword(s):  




2014 ◽  
Vol 38 (2) ◽  
pp. 99-105
Author(s):  
정종원 ◽  
김진수 ◽  
Park Bo ram ◽  
Sugwan Kim ◽  
김춘성 ◽  
...  


2014 ◽  
Vol 117 (5) ◽  
pp. 544-550 ◽  
Author(s):  
Sung-Min Moon ◽  
Jae-Sung Kim ◽  
Heung-Joong Kim ◽  
Mi Suk Choi ◽  
Bo Ram Park ◽  
...  


Mycorrhiza ◽  
2014 ◽  
Vol 24 (6) ◽  
pp. 453-463 ◽  
Author(s):  
Sandra B. Visnovsky ◽  
Nicholas Cummings ◽  
Alexis Guerin-Laguette ◽  
Yun Wang ◽  
Akiyoshi Yamada ◽  
...  


Mycoscience ◽  
2011 ◽  
Vol 52 (5) ◽  
pp. 289-295 ◽  
Author(s):  
Ellen Larsson ◽  
Henrik Sundberg


2010 ◽  
Vol 9 (1) ◽  
pp. 57 ◽  
Author(s):  
Clara Salaheddin ◽  
Yoshimitsu Takakura ◽  
Masako Tsunashima ◽  
Barbara Stranzinger ◽  
Oliver Spadiut ◽  
...  


Sign in / Sign up

Export Citation Format

Share Document