scholarly journals Isolation and some characterization of S-locus-specific glycoproteins associated with self-incompatibility in Brassica campestris.

1986 ◽  
Vol 50 (5) ◽  
pp. 1365-1367 ◽  
Author(s):  
Seiji TAKAYAMA ◽  
Akira ISOGAI ◽  
Chise TSUKAMOTO ◽  
Yukari UEDA ◽  
Kokichi HINATA ◽  
...  
Keyword(s):  
Brassica Campestris ◽  
Self Incompatibility ◽  
S Locus

scholarly journals Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana

2001 ◽  
Vol 13 (3) ◽  
pp. 627-643 ◽  
Author(s):  
Makoto Kusaba ◽  
Kathleen Dwyer ◽  
Jennifer Hendershot ◽  
Julia Vrebalov ◽  
June B. Nasrallah ◽  
...  
Keyword(s):  
Self Incompatibility ◽  
S Locus

scholarly journals Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1587-1597 ◽  
Author(s):  
Katsunori Hatakeyama ◽  
Takeshi Takasaki ◽  
Masao Watanabe ◽  
Kokichi Hinata
Keyword(s):  
Brassica Rapa ◽  
Genomic Dna ◽  
Transmembrane Domain ◽  
Brassica Species ◽  
Self Incompatibility ◽  
S Locus ◽  
Intron Sequence ◽  
High Degree ◽  
Degree Of Similarity

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

scholarly journals Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)

Genetics ◽  
1999 ◽  
Vol 153 (1) ◽  
pp. 391-400 ◽  
Author(s):  
Go Suzuki ◽  
Naoko Kai ◽  
Tamaki Hirose ◽  
Kiichi Fukui ◽  
Takeshi Nishio ◽  
...  
Keyword(s):  
Brassica Campestris ◽  
Protein A ◽  
S Locus ◽  
Cysteine Rich Protein ◽  
Pollen Coat ◽  
Genes Encoding ◽  
Gene Rich Region ◽  
Identification And Characterization ◽  
S Locus Glycoprotein

Abstract In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

The Brassica self-incompatibility multigene family

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 969-972 ◽  
Author(s):  
Kathleen G. Dwyer ◽  
Anna Chao ◽  
Betty Cheng ◽  
Che-Hong Chen ◽  
June B. Nasrallah
Keyword(s):  
Genomic Dna ◽  
Genomic Organization ◽  
Genetic Locus ◽  
Brassica Species ◽  
Self Incompatibility ◽  
S Locus ◽  
S Gene ◽  
Gene Copies ◽  
Genomic Regions

The pollen–stigma interaction of self-incompatibility in Brassica species is controlled by a single genetic locus, the S locus. This locus encodes the S locus specific glycoproteins of the stigma. Hybridization of restriction enzyme digested genomic DNA isolated from homozygous strains of Brassica with cDNA probes encoding these glycoproteins yields a pattern of multiple fragments of varying size and intensity. The presence of S sequences as multiple related copies in the Brassica oleracea genome has been verified by the cloning of several different genomic regions containing S homology. Probes capable of distinguishing between S gene copies have been obtained and have demonstrated that two such copies are expressed. Characterization of additional probes specific for S gene copies will permit the study of the expression of the other copies, and the analysis of the genomic organization of the self-incompatibility multigene family.Key words: self-incompatibility, Brassica, cDNA probes.

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