Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata

Abstract Self-incompatibility in Brassica oleracea is controlled by the highly polymorphic S locus. Isolation and subsequent characterization of the S-locus-glycoprotein (SLG) gene, which encodes the S-locus-specific glycoprotein (SLSG), has revealed the presence of a self-incompatibility multigene family. One of these S-locus-related genes, SLR1, has been shown to be expressed. In this study we present the isolation and preliminary characterization of a second expressed S-locus-related sequence, SLR2. Through restriction fragment length polymorphism (RFLP) linkage analysis we demonstrate that the SLR1 and SLR2 loci reside approximately 18.5 map units apart in one linkage group that segregates independently of the S-locus. The identification of a second SLR gene expressed in stigmas suggests that loci unlinked to the S-locus may play a role in the self-incompatibility response, or in pollination in general.

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Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars

International Journal of Horticultural Science ◽

10.31421/ijhs/20/3-4/1128 ◽

2014 ◽

Vol 20 (3-4) ◽

Cited By ~ 3

Author(s):

J. Halász ◽

A. Kurilla ◽

A. Hegedűs

Keyword(s):

Genetic Diversity ◽

Unknown Origin ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

Prunus Domestica ◽

High Genetic Diversity ◽

First Intron ◽

S Alleles

European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the second intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.

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Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽

10.3390/plants10102186 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2186

Author(s):

Zhiquan Liu ◽

Bing Li ◽

Yong Yang ◽

Changbin Gao ◽

Bin Yi ◽

...

Keyword(s):

Brassica Napus ◽

Genetic Locus ◽

Recognition System ◽

Inbred Lines ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

S Haplotype ◽

Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

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Molecular Characterization of a 313-kb Genomic Region Containing the Self-incompatibility Locus of Ipomoea trifida, a Diploid Relative of Sweet Potato

Breeding Science ◽

10.1270/jsbbs.54.165 ◽

2004 ◽

Vol 54 (2) ◽

pp. 165-175 ◽

Cited By ~ 15

Author(s):

Rubens Norio Tomita ◽

Go Suzuki ◽

Kazuo Yoshida ◽

Yukihito Yano ◽

Tohru Tsuchiya ◽

...

Keyword(s):

Molecular Characterization ◽

Sweet Potato ◽

Genomic Region ◽

The Self ◽

Self Incompatibility ◽

Ipomoea Trifida ◽

Incompatibility Locus

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Molecular characterization of the S locus in two self-incompatible Brassica napus lines.

The Plant Cell ◽

10.1105/tpc.8.12.2369 ◽

1996 ◽

Vol 8 (12) ◽

pp. 2369-2380 ◽

Cited By ~ 33

Author(s):

K Yu ◽

U Schafer ◽

T L Glavin ◽

D R Goring ◽

S J Rothstein

Keyword(s):

Brassica Napus ◽

Molecular Characterization ◽

S Locus

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Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana

The Plant Cell ◽

10.1105/tpc.13.3.627 ◽

2001 ◽

Vol 13 (3) ◽

pp. 627-643 ◽

Cited By ~ 181

Author(s):

Makoto Kusaba ◽

Kathleen Dwyer ◽

Jennifer Hendershot ◽

Julia Vrebalov ◽

June B. Nasrallah ◽

...

Keyword(s):

Self Incompatibility ◽

S Locus

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Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmz057 ◽

2019 ◽

Vol 51 (7) ◽

pp. 723-733 ◽

Cited By ~ 1

Author(s):

Songmei Shi ◽

Qiguo Gao ◽

Tonghong Zuo ◽

Zhenze Lei ◽

Quanming Pu ◽

...

Keyword(s):

Brassica Oleracea ◽

Signaling Pathway ◽

Chromosomal Region ◽

The Self ◽

Mature Stage ◽

Self Incompatibility ◽

Receptor Kinase ◽

S Locus ◽

Phylogenic Tree ◽

Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

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Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L

Genetics ◽

10.1093/genetics/149.3.1587 ◽

1998 ◽

Vol 149 (3) ◽

pp. 1587-1597 ◽

Cited By ~ 5

Author(s):

Katsunori Hatakeyama ◽

Takeshi Takasaki ◽

Masao Watanabe ◽

Kokichi Hinata

Keyword(s):

Brassica Rapa ◽

Genomic Dna ◽

Transmembrane Domain ◽

Brassica Species ◽

Self Incompatibility ◽

S Locus ◽

Intron Sequence ◽

High Degree ◽

Degree Of Similarity

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

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