Faculty Opinions recommendation of Identification and characterization of components of a putative petunia S-locus F-box-containing E3 ligase complex involved in S-RNase-based self-incompatibility.

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

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Identification and characterization of pin and thrum alleles of two genes that co-segregate with the Primula S locus

The Plant Journal ◽

10.1111/j.1365-313x.2007.03125.x ◽

2007 ◽

Vol 51 (1) ◽

pp. 18-31 ◽

Cited By ~ 35

Author(s):

Jinhong Li ◽

Margaret Webster ◽

Masaki Furuya ◽

Philip M. Gilmartin

Keyword(s):

S Locus ◽

Identification And Characterization

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Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata

Plant Molecular Biology ◽

10.1023/b:plan.0000019068.00034.09 ◽

2003 ◽

Vol 53 (4) ◽

pp. 565-580 ◽

Cited By ~ 46

Author(s):

Yan Wang ◽

Xi Wang ◽

Andrew G. McCubbin ◽

Teh-hui Kao

Keyword(s):

Genetic Mapping ◽

Molecular Characterization ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

Petunia Inflata

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Identification and characterization of newS-alleles associated with self-incompatibility in almond

Plant Breeding ◽

10.1111/j.1439-0523.2008.01541.x ◽

2008 ◽

Vol 127 (6) ◽

pp. 632-638 ◽

Cited By ~ 14

Author(s):

O. Kodad ◽

A. Sánchez ◽

N. Saibo ◽

M. Oliveira ◽

R. Sociasi Company

Keyword(s):

Self Incompatibility ◽

Identification And Characterization

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Characterization of a putative S-locus encoded receptor protein kinase and its role in self-incompatibility. Progress report

10.2172/10148217 ◽

1994 ◽

Author(s):

J.B. Nasrallah

Keyword(s):

Protein Kinase ◽

Progress Report ◽

Receptor Protein ◽

Self Incompatibility ◽

S Locus

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The S –locus and unilateral incompatibility

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2003.1284 ◽

2003 ◽

Vol 358 (1434) ◽

pp. 1133-1140 ◽

Cited By ~ 39

Author(s):

C. Nathan Hancock ◽

Katsuhiko Kondo ◽

Brian Beecher ◽

Bruce McClure

Keyword(s):

Control Mechanisms ◽

S Locus ◽

Pollen Rejection ◽

Stigma Surface ◽

Allele Specific ◽

Genetic Mechanisms ◽

Active Research ◽

Compatible Pollen ◽

Identification And Characterization

Plants have many ways to regulate the type of pollen that arrives on the stigma surface. Once there, further control mechanisms regulate compatibility. The latter controls are largely based on biochemical interactions that support compatible pollination and prevent incompatible matings. S–RNase–based self–incompatibility (SI) systems are the most phylogenetically widespread mechanisms for controlling pollination. Studies of Nicotiana establish a firm link between SI and unilateral interspecific incompatibility. Although implicated in both inter– and intraspecific compatibility, S–RNase operates through at least three distinct genetic mechanisms that differ in their dependence on non–S–RNase factors. Identification and characterization of these non–S–RNase factors is currently an area of active research. Searching for genetic and biochemical interactions with S–RNase can identify candidate non–S–RNase factors. HT–protein is one factor that is required for S –allele–specific pollen rejection in the Solanaceae. Major style arabinogalactan proteins such as TTS interact biochemically with S–RNase. These glycoproteins are known to interact with compatible pollen tubes and have long been suggested as possible recognition molecules. Their binding to S–RNase implies a link between stylar systems for compatibility and incompatibility. Thus, genetic and biochemical studies suggest a highly networked picture of pollen–pistil interactions.

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Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)

Genetics ◽

10.1093/genetics/153.1.391 ◽

1999 ◽

Vol 153 (1) ◽

pp. 391-400 ◽

Cited By ~ 7

Author(s):

Go Suzuki ◽

Naoko Kai ◽

Tamaki Hirose ◽

Kiichi Fukui ◽

Takeshi Nishio ◽

...

Keyword(s):

Brassica Campestris ◽

Protein A ◽

S Locus ◽

Cysteine Rich Protein ◽

Pollen Coat ◽

Genes Encoding ◽

Gene Rich Region ◽

Identification And Characterization ◽

S Locus Glycoprotein

Abstract In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.

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