In Vitro Maturation of Human Immature Oocytes in Culture Medium Supplemented with Patient's Own Serum or Donor Follicular Fluid

2008 ◽  
Vol 25 (3) ◽  
pp. 163-166
Author(s):  
Hiroyuki Kanaya ◽  
Aisaku Fukuda ◽  
Shu Hashimoto ◽  
Yoshihiko Hosoi ◽  
Yoshiharu Morimoto
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Immature Oocytes

188 HUMAN FOLLICULAR FLUID IN EQUINE IN VITRO MATURATION MEDIUM SUPPORTS IN VITRO MATURATION AND VIABLE INTRACYTOPLASMIC SPERM INJECTION-BLASTOCYST PREGNANCIES

2012 ◽  
Vol 24 (1) ◽  
pp. 206
Author(s):  
C. Makloski ◽  
R. Gotti ◽  
K. Harris ◽  
J. Bottger ◽  
M. Meintjes
Keyword(s):  
Follicular Fluid ◽  
Intracytoplasmic Sperm Injection ◽  
In Vitro Maturation ◽  
Transvaginal Ultrasound ◽  
Human Follicular Fluid ◽  
Immature Oocytes ◽  
Treatment Groups ◽  
Maturation Medium ◽  
And Control

The aim of this study was 2-fold: (1) To determine if altrenogest-treated mares will yield higher numbers of quality in vitro-matured (IVM) oocytes than early pregnant mares and cycling/control mares and (2) if the addition of human follicular fluid (HFF) to IVM medium can support IVM and viable pregnancies from in vitro-produced blastocysts. In this study, 18 mares were assigned to 3 equally sized treatment groups and each mare was subjected to follicle aspiration every 10 to 11 days without monitoring follicular growth. The 3 treatment groups were altrenogest-treated mares (0.044 mg kg–1 of PO daily), early pregnant mares (30–110 days) and control/cycling mares. Using transvaginal ultrasound guidance, all visible follicles were aspirated. Altrenogest-treated mares each yielded more follicles (8.75) per aspiration session when compared with the control mare group (5.75) and the pregnant mare group (3.72), but there was no difference in oocyte recovery rates among the groups (Table 1). A limited number of these oocytes were subjected to in vitro maturation. After heated (38.5°C) transport of oocytes to an off-site laboratory, the oocytes were placed in maturation medium containing 10% HFF obtained from preovulatory follicles after ovulation induction, 20% serum substitute supplement and no hormones for 36 h. This approach yielded a maturation rate of 61.8, 68.8 and 82.0% for the altrenogest, pregnant and control treatment groups, respectively (not significant). Mature oocytes (n = 65) were injected with frozen-thawed sperm using a standard intracytoplasmic sperm injection (ICSI) technique. Four expanding blastocysts (Table 1) were selectively transported back to the embryo transfer facility and transcervically transferred into recipient mares on Day 6 post-ICSI. These 4 transfers resulted in 2 viable, normally progressing pregnancies, ongoing beyond 60 days of gestation. Both pregnancies resulted from the altrenogest-treated aspiration group. In this study we concluded that (1) altrenogest-treated mares provide more follicles and may be a better source of viable immature oocytes for the production of ICSI embryos and foals, but their overall advantage is unclear; (2) addition of HFF to IVM media, in the absence of added gonadotropins, can support oocyte maturation, blastocyst production and viable pregnancies; (3) an aspiration schedule of every 10 to 11 days without ultrasonic monitoring can yield viable immature oocytes, capable of producing ICSI blastocysts, resulting in viable pregnancies. Table 1.Altrenogest-treated mares compared to early pregnant mares and control mares

scholarly journals ROLE OF SMART MEDIUM SUPPLEMENTED WITH FOLLICULAR FLUID IN IN VITRO MATURATION FOR SHEEP IMMATURE OOCYTES RECOVERED FROM XENOTRANSPLANTED OVARIAN TISSUE CORTEX.

2016 ◽  
Vol 4 (10) ◽  
pp. 1295-1300
Author(s):  
Prof.Dr.MuhammadBaqir M.R.Fakhrildin ◽  
◽  
Dr.NadiaJ.Sudani Al-Behadili ◽  
Prof.Dr.Mohammad OdaSelman. ◽  
◽  
...  
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Ovarian Tissue ◽  
Immature Oocytes

scholarly journals Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 883 ◽  
Author(s):  
Pablo Fernández-Hernández ◽  
María Jesús Sánchez-Calabuig ◽  
Luis Jesús García-Marín ◽  
María J. Bragado ◽  
Alfonso Gutiérrez-Adán ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Creatine Phosphate ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Successful Pregnancy ◽  
The Mean ◽  
Mean Concentration

Production of equine embryos in vitro is currently a commercial technique and a reliable way of obtaining offspring. In order to produce those embryos, immature oocytes are retrieved from postmortem ovaries or live mares by ovum pick-up (OPU), matured in vitro (IVM), fertilized by intracytoplasmic sperm injection (ICSI), and cultured until day 8–10 of development. However, at best, roughly 10% of the oocytes matured in vitro and followed by ICSI end up in successful pregnancy and foaling, and this could be due to suboptimal IVM conditions. Hence, in the present work, we aimed to elucidate the major metabolites present in equine preovulatory follicular fluid (FF) obtained from postmortem mares using proton nuclear magnetic resonance spectroscopy (1H-NMR). The results were contrasted against the composition of the most commonly used media for equine oocyte IVM: tissue culture medium 199 (TCM-199) and Dulbecco’s modified eagle medium/nutrient mixture F-12 Ham (DMEM/F-12). Twenty-two metabolites were identified in equine FF; among these, nine of them are not included in the composition of DMEM/F-12 or TCM-199 media, including (mean ± SEM): acetylcarnitine (0.37 ± 0.2 mM), carnitine (0.09 ± 0.01 mM), citrate (0.4 ± 0.04 mM), creatine (0.36 ± 0.14 mM), creatine phosphate (0.36 ± 0.05 mM), fumarate (0.05 ± 0.007 mM), glucose-1-phosphate (6.9 ± 0.4 mM), histamine (0.25 ± 0.01 mM), or lactate (27.3 ± 2.2 mM). Besides, the mean concentration of core metabolites such as glucose varied (4.3 mM in FF vs. 5.55 mM in TCM-199 vs. 17.5 mM in DMEM/F-12). Hence, our data suggest that the currently used media for equine oocyte IVM can be further improved.

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