Effect of Growth Regulator, Sucrose, and Minimal-growth Conservation on In Vitro Propagation of Virus-free Sweet Potato Plantlets

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L−1 NAA and 1 mg L−1 BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L−1 NAA and 1 mg L−1 BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L−1 NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L−1 NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue. Keywords: Bulb, lily, micro bulbs, in vitro, shoots

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COMPARATIVE ULTRASTRUCTURES OF VITREOUS AND NON-VITREOUS SWEET POTATO (IPOMOEA BATATAS L.)LAM. PLANTS GROWN IN VITRO

HortScience ◽

10.21273/hortsci.25.8.860g ◽

1990 ◽

Vol 25 (8) ◽

pp. 860g-860

Author(s):

Nenita V. Desamero ◽

Billy B. Rhodes

Keyword(s):

Cell Wall ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Membrane Separation ◽

Culture Conditions ◽

Physiological Disorder ◽

Stomatal Complex ◽

Single Node ◽

Potato Plantlets

Vitrification, a physiological disorder characteristic of in vitro grown plants, was observed in single-node cultures of sweet potato in mannitol-enriched medium during their second year of storage. Vitrified or vitreous sweet potato plantlets were watersoaked, translucent or glassy in appearance, with thick, swollen, leaves and stems, stunted shoot growth and poor root growth. These plantlets were not able to regenerate normal plants when transferred into fresh regeneration medium nor were they able to survive outside culture conditions. Electron microscopy revealed changes in the ultrastructures of vitrified sweet potato plantlets. Vitrified plants had defective stomatal complex, starch grain-filled chloroplasts, disrupted cell wall, big air spaces (lacunae), high frequency of cell membrane separation from the cell wall, nuclear disintegration, and cytoplasmic disorganization. These changes in the internal structures of vitrified plants were reflected in their abnormal morphology and physiology.

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THE USE OF IN VITRO MIST CULTIVATION FOR THE PROPAGATION AND HARDENING-OFF OF VIRUS-FREE POTATO PLANTLETS.

HortScience ◽

10.21273/hortsci.27.6.698b ◽

1992 ◽

Vol 27 (6) ◽

pp. 698b-698

Author(s):

Kenneth L. Giles ◽

Kenneth Friesen ◽

Debra Novakovski

Keyword(s):

Growth Regulator ◽

High Efficiency ◽

Shoot Multiplication ◽

Culture Vessel ◽

Skoog Medium ◽

Murashige And Skoog Medium ◽

Nodal Cuttings ◽

Mineral Concentrations ◽

Potato Plantlets

The use of an in vitro mist culture systems for the growth of virus free shoots of several potato cultivars has been demonstrated using the “Mystifier®”, several different media and growth regulator concentrations have been used to monitor the efficiency of shoot multiplication and growth. Nodal cuttings of sterile in vitro virus free cultures were used as inocula and the mist was applied at various rates in order to assess optimum sucrose concentrations and mineral concentrations. Murashige and Skoog medium was used and diluted to half and quarter strength. Hardening off the resulting shoots was achieved by passing HEPA filtered air at various rates over the plantlets still in the culture vessel. Conditions for high efficiency hardening off of cultured material has been defined such that plantlets can be transferred directly to greenhouse conditions. The implications and opportunities that are indicated by this work are discussed.

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In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.)

сборник статей всероссийской научной конференции с международным участием "Растениеводство и луговодство" ◽

10.26897/978-5-9675-1762-4-2020-104 ◽

2020 ◽

Author(s):

H. G. Abubakarov ◽

◽

E.A. Kalashnikova ◽

R.N. Kirakosyan ◽

A.V. Shitikova

Keyword(s):

Sweet Potato ◽

Mineral Composition ◽

Nutrient Medium ◽

In Vitro Propagation ◽

Ipomoea Batatas ◽

Sweet Potatoes ◽

Simultaneous Formation

The results of optimizing the conditions for growing sweet potatoes in vitro are presented. It is shown that the mineral composition of the nutrient medium has a significant effect on the growth of side shoots. With the simultaneous formation of shoots, rooting of micro gears was observed.

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