Specific IgE Immunoassay for House Dust Mites by Using Multiple Allergen Simultaneous Test (MAST) in Asthmatic Children

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

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179 Sensitization to house dust mites in asthmatic children in Argentina and cross-reactivity studies

Journal of Allergy and Clinical Immunology ◽

10.1016/s0091-6749(96)80397-6 ◽

1996 ◽

Vol 97 (1) ◽

pp. 227-227

Author(s):

C BAENACAGNANI ◽

E FERNANDEZCALDAS ◽

L ARLIAN ◽

R CODINA ◽

M CUELLO ◽

...

Keyword(s):

House Dust ◽

Cross Reactivity ◽

House Dust Mites ◽

Dust Mites ◽

Asthmatic Children

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Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

BMC Veterinary Research ◽

10.1186/s12917-021-02768-2 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

N. Khantavee ◽

C. Chanthick ◽

A. Tungtrongchitr ◽

N. Techakriengkrai ◽

S. Suradhat ◽

...

Keyword(s):

House Dust ◽

Characteristic Curve ◽

False Positive Rate ◽

Specific Ige ◽

House Dust Mites ◽

Dust Mites ◽

Dermatophagoides Farinae ◽

Allergen Specific Immunotherapy ◽

Intradermal Testing ◽

Positive Rate

Abstract Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy (n = 33) and atopic dogs (AD) (n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p = 1.00). High levels of all antibody isotypes (IgE, IgG, IgG1 and IgG2) against HDM were found in aged healthy dogs (> 3 years old). In AD, HDM-IgE and IgG1 levels were higher in dogs that were IDT positive to HDM than in IDT negative animals. Levels of IgE and IgG1 could be used to distinguish the specific allergens, whereas total IgG and IgG2 levels were not different between IDT-positive and IDT-negative AD. By the receiver operating characteristic curve at a false-positive rate = 0.10, both IgE and IgG1 showed better sensitivity than IgG and IgG2. Similar to IgE, serum IgG1 concentration was also relevant to IDT outcomes. Conclusions Our in-house ELISAs coated with local HDM were useful for evaluating antibody levels, and we propose use of the HDM-specific IgG1 subclass as a biomarker to detect HDM specific allergens in AD, potentially together with an IgE based platform.

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Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E Detection in Patients with Isolated Allergic Conjunctivitis

Journal of Clinical Medicine ◽

10.3390/jcm10050960 ◽

2021 ◽

Vol 10 (5) ◽

pp. 960

Author(s):

Jung Yeob Han ◽

Hun Lee ◽

Jae Lim Chung ◽

Young Jun Kim ◽

Jae Yong Kim ◽

...

Keyword(s):

House Dust ◽

Allergic Conjunctivitis ◽

Immunoglobulin E ◽

Age Groups ◽

Specific Ige ◽

House Dust Mites ◽

Detection Rates ◽

Immunoblot Assay ◽

Simultaneous Test ◽

Positive Rate

We aimed to investigate the immunoglobulin E (IgE) detection rate and allergen patterns in patients with isolated allergic conjunctivitis using the multiple allergen simultaneous test (MAST)-immunoblot assay. A total of 120 patients with allergic conjunctivitis and no associated rhinitis, asthma, or dermatitis underwent the MAST-immunoblot assay to measure serum total IgE (TIgE) and serum specific IgE (SIgE) against 57 allergens. Patients were classified into subgroups based on the season when the eye symptoms were exacerbated, and TIgE and SIgE positivity. Differences between sex and age groups were also analyzed. Of the 120 patients, 57.5% (69 patients) and 69.2% (83 patients) were positive for TIgE (≥100 IU/mL) and SIgE (≥0.7 IU/mL), respectively. The allergens that most frequently triggered sensitization in the study population were Dermatophagoides farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Alternaria, and house dust. House dust mites, such as D. farinae and D. pteronyssinus, showed the highest detection rates regardless of the season. Men had a higher positive rate for TIgE than women, whereas a higher rate of sensitization, detected as SIgE positivity, was seen in younger patients. In conclusion, MAST-immunoblot assay can detect sensitizing allergens in patients with isolated allergic conjunctivitis.

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Allergic rhinitis complicated by hypertrophy of the mucous membrane of nasal turbinates in patients of Northern Vietnam

Biomedical Research and Therapy ◽

10.15419/bmrat.v7i6.609 ◽

2020 ◽

Vol 7 (6) ◽

pp. 3813-3818

Author(s):

Kryukov Andrei Ivanovich ◽

Galina Petrovna Bondareva ◽

Nguyen Thi Phuong Thao

Keyword(s):

Allergic Rhinitis ◽

Nasal Cavity ◽

Mucous Membrane ◽

House Dust ◽

Inferior Turbinate ◽

Specific Ige ◽

House Dust Mites ◽

Moderate Degree ◽

Dust Mites ◽

Northern Vietnam

Introduction: Constant contact with allergens contributes to the formation of allergic rhinitis and swelling of the mucous membrane of the nasal cavity. Moreover, constant inflammation stimulates hypertrophy of the mucous membrane of the nasal turbinates and paranasal sinuses. This study aimed to assess the clinical features of hypertrophic rhinitis (HR) in combination with allergic rhinitis (AR) in patients from Northern Vietnam to improve the effectiveness of treatment and quality of life of patients with this pathology of the nasal cavity. Methods: The study was conducted from June to September 2018 by the Department of Otorhinolaryngology, Thai Nguyen Central General Hospital, Vietnam. A total of 158 patients with symptoms of chronic rhinitis, aged 5–70 years, were examined. All patients with allergic rhinitis were subjected to standard, specific allergological tests, such as the determination of specific IgE in the blood, and statistics were assessed according to the otorhinolaryngology examination and CT scan. All patients were also examined by otolaryngologists. Results: According to the results of our study, among 64 patients with a diagnosis of AR, a total of 45 (70.31%) patients were diagnosed as AR in combination with a hypertrophy of the mucous membrane of the nasal turbinate, with an average duration of morbidity of 5.8 ±1.6 years. Moreover, 17 (37.78%) patients were diagnosed with severe hypertrophy of the mucous membrane of the nasal inferior turbinate (the nasal inferior turbinate is 50% or more of nasal cavity and with nasal polyps). The sensitization to house dust mites and fungi was high. Conclusion: The peculiar findings of AR in combination with HR detected in patients of Northern Vietnam was associated with the onset of the disease at the age of 20-40 years, the predominance in men, the predominance of the disease in smokers, and the predominance in residents of cities and industrial areas (71.1%). Indeed, there was an observed combination of AR with severe and moderate degree of HR. The sensitization of patients with AR in combination with HR in Northern Vietnam are similar to those in Asia, with predominant allergy to house dust mites.

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Association between house dust mites sensitization and level of asthma control and severity in children attending Mansoura University Children’s Hospital

Egyptian Journal of Bronchology ◽

10.1186/s43168-021-00082-x ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Nahla Mohamed Okasha ◽

Amr Ali Sarhan ◽

Engy Osman Ahmed

Keyword(s):

Asthma Control ◽

House Dust ◽

Asthma Severity ◽

House Dust Mites ◽

Dust Mites ◽

Asthma Phenotype ◽

Asthmatic Children ◽

Significant Difference ◽

Control Asthma ◽

Gina Guidelines

Abstract Background House dust mites (HDM) are considered as a major source of indoor aeroallergen all over the world that precipitate allergic reactions including rhinoconjunctivitis, food allergy, atopic dermatitis, and allergic asthma. In this study, we aimed to assess the clinical and laboratory profile of some Egyptian asthmatic children who are sensitized to house dust mites and determine the association of HDM sensitization and severity of asthma according to recent GINA guidelines. Results The most frequent clinical phenotype among the HDM-positive group was a cough (96.7%), while the clinical phenotypic wheeze was frequent in HDM negative group (96.7%). There is a statistically significant difference between studied cases classified according to sensitivity to HDM regarding level of asthma control (50.0% were uncontrolled among cases sensitive to HDM and 56.7% of cases non-sensitive to HDM were controlled (p = 0.001*). Severity according to treatment results was significantly higher among cases sensitive to HDM (26.7% of cases sensitive to HDM versus 3.3% of cases non-sensitive to HDM), p = 0.017. Conclusions HDM sensitization is associated mainly with cough asthma phenotype and allergic rhinitis in our studied cases. HDM sensitization increases asthma severity and decreases the ability to control asthma symptoms. Trial registration ClinicalTrial.gov, NCT04958616.

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MITE ASTHMA IN CHILDHOOD: A STUDY OF THE RELATIONSHIP BETWEEN EXPOSURE TO HOUSE DUST MITES AND DISEASE ACTIVITY

PEDIATRICS ◽

10.1542/peds.94.2.250b ◽

1994 ◽

Vol 94 (2) ◽

pp. 250-251

Author(s):

Martin Sachs

Keyword(s):

House Dust ◽

Respiratory Infections ◽

Mite Allergen ◽

House Dust Mites ◽

Dust Mites ◽

Asthmatic Children ◽

Allergic Asthmatic ◽

Viral Respiratory Infections ◽

The Relationship ◽

Viral Respiratory

When mite allergic asthmatic children are exposed to high levels of mite allergen, the number of mites in the mattress dust does not correlate with increased symptoms. The authors further suggested that other factors (eg, viral respiratory infections) are more likely to be associated with increased symptoms.

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