Different Detection Methods of Virulent Helicobacter Pylori in Gastric Biopsies

Abstract The continuous rise in the number of patients suffering from Helicobacter pylori is probably due to the changes in modern life. Nowadays patients suffering from gastrointestinal problems are diagnosed through invasive and noninvasive techniques. The choice of a diagnostic test is influenced by factors such as the tests' sensitivity and specificity, the clinical conditions, and the cost-effectiveness of the testing strategy. This study aimed to compare molecular detection methods of H. pylori by polymerase chain reaction (PCR) targeting the 16S rRNA, ureA and glmM genes with an invasive histopathological technique. A total of 290 patients from various hospitals in Khartoum State were recruited in this study between March 2018 and January 2020. Two gastric biopsies were collected from each patient for PCR and histopathology.A total of 103 (35.5%) samples were positive by histopathological examination, 88 (30.3%) by 16SrRNA, 39 (13.4%) by glmM gene, and 56 (19.3%) by ureA gene. The highest sensitivity was observed in 16SrRNA (46.6%), followed by ureA (35%), and glmM (24.3%). While the best specificity was observed in glmM gene (92.5%), followed by ureA (89.3%) and 16SrRNA (78.6%). In conclusion, the PCR test targeting the16SrRNA gene exhibited the best results for molecular detection of H. pylori compared to other genes.

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Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

Infectious Disorders - Drug Targets ◽

10.2174/1871526518666180515113218 ◽

2019 ◽

Vol 19 (3) ◽

pp. 274-278 ◽

Cited By ~ 1

Author(s):

Saba Fakhrieh Asl ◽

Mehrnaz Pourvahedi ◽

Ali Mojtahedi ◽

Mohammad Shenagari

Keyword(s):

Helicobacter Pylori ◽

Gastric Diseases ◽

Specific Primers ◽

The North ◽

Different Types ◽

North Of Iran ◽

Gastric Biopsies ◽

Pcr Method ◽

Non Ulcer Dyspepsia ◽

H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

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DETECTION OF HELICOBACTER PYLORI cagA GENE IN GASTRIC BIOPSIES, CLINICAL ISOLATES AND FAECES

Indian Journal of Medical Microbiology ◽

10.1016/s0255-0857(21)02349-5 ◽

2006 ◽

Vol 24 (3) ◽

pp. 195-200

Author(s):

KM Bindayna ◽

WAAl Baker ◽

GA Botta

Keyword(s):

Helicobacter Pylori ◽

Clinical Isolates ◽

Caga Gene ◽

Gastric Biopsies

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M1088 Rapid Molecular Assay for the Diagnosis of Clarithromycin Resistant Helicobacter pylori Directly from Human Gastric Biopsies-An Approach to Design Antibiotic Regimen

Gastroenterology ◽

10.1016/s0016-5085(08)61564-4 ◽

2008 ◽

Vol 134 (4) ◽

pp. A-335

Author(s):

Chittor M. Habibullah ◽

Santosh K. Tiwari ◽

Md. Aejaz Habeeb ◽

Manoj Gopi ◽

Shakeel Ahmed ◽

...

Keyword(s):

Helicobacter Pylori ◽

Antibiotic Regimen ◽

Molecular Assay ◽

Gastric Biopsies

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Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

10.51429/ejmm29308 ◽

2020 ◽

Vol 29 (3) ◽

pp. 59-64

Author(s):

Hanaa M. El Maghraby ◽

Samar Mohaseb

Keyword(s):

Helicobacter Pylori ◽

Epigastric Pain ◽

Deletion Mutation ◽

Rapid Urease Test ◽

Rrna Gene ◽

University Hospitals ◽

Metronidazole Resistance ◽

Urease Test ◽

Gastric Biopsies ◽

H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

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Assessing the Reliability of PCR, Microbiological and Histologic Methods in Detecting Helicobacter pylori in Gastric Biopsies

Journal of Biomedical Sciencies ◽

10.4172/2254-609x.100076 ◽

2017 ◽

Vol 06 (04) ◽

Cited By ~ 1

Author(s):

Yaji Mnena E ◽

Nna Emmanuel ◽

Ega RAI

Keyword(s):

Helicobacter Pylori ◽

Gastric Biopsies

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Isolation and characterization of Helicobacter pylori recovered from gastric biopsies under anaerobic conditions

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2016.07.009 ◽

2016 ◽

Vol 86 (2) ◽

pp. 136-140 ◽

Cited By ~ 4

Author(s):

Guillerm Ignacio Perez-Perez ◽

Thinh Nguyen Van ◽

Duong Thu Huong ◽

Gao Zhan ◽

Do Nguyet Anh ◽

...

Keyword(s):

Helicobacter Pylori ◽

Anaerobic Conditions ◽

Isolation And Characterization ◽

Gastric Biopsies

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Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies

Malaysian Journal of Medical Sciences ◽

10.21315/mjms2020.27.5.6 ◽

2020 ◽

Vol 27 (5) ◽

pp. 53-61

Author(s):

Abdullah Saleh Alkhamiss

Keyword(s):

Helicobacter Pylori ◽

Alcian Blue ◽

National Guard ◽

Periodic Acid ◽

Giemsa Stain ◽

Periodic Acid Schiff ◽

Gastric Biopsies ◽

Hematoxylin And Eosin ◽

H Pylori ◽

Sensitivity Specificity

Background: This study was undertaken to evaluate the preferred method (Giemsa or periodic acid Schiff-Alcian blue [PAS-AB] stains) of detecting Helicobacter pylori (H. pylori) in gastric mucosal biopsies in terms of sensitivity, specificity and applicability. To the best of my knowledge, this is the first report comparing Giemsa and PAS-AB staining for the detection of H. pylori in such biopsies. Methods: The formalin-fixed paraffin-embedded blocks of 49 gastric biopsies from different patients were collected from the archive of anatomical pathology at King Abdulaziz Medical City, National Guard, Riyadh, Saudi Arabia. From each block, three slides were prepared and analysed using the hematoxylin and eosin (H&E), Giemsa and PAS-AB stains to detect the presence/absence of H. pylori, and the results were compared in terms of sensitivity, specificity and applicability. Results: The majority of the biopsies in this study showed antrum-type gastric mucosa. Only 15 biopsies showed active gastritis, whereas the rest showed chronic gastritis. Three biopsies showed intestinal metaplasia. All were detected by PAS-AB stain, but only two-thirds were detected by H&E stain. Fifteen gastric biopsies showed H. pylori infection in general and in 13 of them, active gastritis cases were discovered. Fourteen out of these 15 H. pylori infection cases were detected by Giemsa stain, whereas only 13 cases were detected by H&E stain. PAS-AB stain showed the worst results since it demonstrated only 40% sensitivity and 67.65% specificity in H. pylori detection. Conclusion: Giemsa stain has better sensitivity and specificity in gastric H. pylori infection detection than PAS-AB. Therefore, using PAS-AB stain to detect H. pylori infection is not recommended.

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