scholarly journals Prevalence and Characterization of Foodborne Pathogens in Dairy Cattle in the Eastern Part of Japan

2013 ◽  
Vol 75 (4) ◽  
pp. 543-546 ◽  
Author(s):  
Yoshimasa SASAKI ◽  
Mariko MURAKAMI ◽  
Mika HARUNA ◽  
Noriko MARUYAMA ◽  
Tetsuya MORI ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Foodborne Pathogens

scholarly journals Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Celosia Lukman ◽  
Christopher Yonathan ◽  
Stella Magdalena ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
High Efficiency ◽  
Multiplicity Of Infection ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Pathogenic Escherichia Coli ◽  
Family Based

Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.

1063 The characterization of estradiol concentration before insemination and its effect on fertility in dairy cattle

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 509-510
Author(s):  
M. Gobikrushanth ◽  
P. A. Dutra ◽  
C. A. Felton ◽  
T. C. Bruinjé ◽  
M. G. Colazo ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Estradiol Concentration

Molecular methods for the detection and characterization of foodborne pathogens

2010 ◽  
Vol 82 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Xian-Ming Shi ◽  
Fei Long ◽  
Biao Suo
Keyword(s):  
Foodborne Pathogens ◽  
Biochemical Characterization ◽  
Molecular Methods ◽  
Nucleic Acid Hybridization ◽  
Food Industries ◽  
Restriction Length Polymorphism ◽  
Polymerase Chain ◽  
Commercial Applications ◽  
Restriction Length

The surveillance of foodborne pathogens in food industries has shown the urgent need for rapid and dependable methods to detect and characterize the organisms in food and environments of clinical and epidemiologic importance. Recent studies on rapid methods in microbiology have been focused on biochemical characterization, immunoassays, and molecular methods. Many molecular methods have been developed and applied to the detection and characterization of foodborne pathogens in laboratories and food industries. They can be mainly divided into DNA banding pattern-based tests and DNA sequence-based tests. The former includes nucleic acid hybridization, polymerase chain reaction (PCR), amplified restriction length polymorphism, and randomly amplified polymorphic DNA, etc. Most of these methods in commercial applications are based on PCR or hybridization techniques. The principle, characteristics, and application of molecular methods for the detection and characterization of foodborne pathogens were reviewed in this article.

scholarly journals Short communication: Characterization of Staphylococcus aureus from bulk tank milk of dairy cattle in Lombardy (northern Italy)

2020 ◽  
Vol 103 (3) ◽  
pp. 2685-2692
Author(s):  
A. Gazzola ◽  
A.M. Maisano ◽  
V. Bianchini ◽  
F. Vezzoli ◽  
A. Romanò ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Short Communication ◽  
Northern Italy ◽  
Bulk Tank Milk ◽  
Bulk Tank

scholarly journals Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 62
Author(s):  
Yilin Wang ◽  
Jianke Cao ◽  
Yankai Chang ◽  
Fuchang Yu ◽  
Sumei Zhang ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Ribosomal Rna ◽  
Triosephosphate Isomerase ◽  
Giardia Duodenalis ◽  
Northwest China ◽  
Small Subunit ◽  
Gastrointestinal Parasites ◽  
Rrna Gene ◽  
And Control

Cryptosporidium spp. and Giardia duodenalis are common gastrointestinal parasites with a broad range of hosts, including humans, livestock, and wildlife. To examine the infection status and assess the zoonotic potential of Cryptosporidium spp. and G. duodenalis in dairy cattle in Gansu, China, a total of 1414 fecal samples were collected from the rectum, with one sample collected from each individual animal. All the samples were tested using nested PCR based on the small subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp. and G. duodenalis. The overall infection rates of Cryptosporidium spp. and Giardia duodenalis were 4.2% (n = 59) and 1.0% (n = 14), respectively. Four Cryptosporidium species were identified: C. andersoni (n = 42), C. parvum (n = 12), C. bovis (n = 5), and C. ryanae (n = 1). In further analyses of subtypes of C. parvum isolates based on the 60 kDa glycoprotein (gp60) gene, five were successfully subtyped as IIdA19G1 (n = 4) and IIdA15G1 (n = 1). All 14 G. duodenalis isolates were identified as assemblage E using the triosephosphate isomerase (tpi) gene. The relatively low positive rates of Cryptosporidium spp. and G. duodenalis detected here and the predominance of non-human pathogenic species/assemblages of these parasites indicated their unique transmission dynamics in this area and the low level of threat posed to public health. However, continuous monitoring and further studies of these parasites should be conducted for the prevention and control of these pathogens.

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