scholarly journals Application of Bacillus subtilis as a live vaccine vector: A review

2020 ◽  
Vol 82 (11) ◽  
pp. 1693-1699
Author(s):  
Penghao LV ◽  
Yanying SONG ◽  
Cong LIU ◽  
Lanping YU ◽  
Yingli SHANG ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Live Vaccine ◽  
Vaccine Vector

scholarly journals Evaluation of Toxoplasma gondii as a live vaccine vector in susceptible and resistant hosts

2011 ◽  
Vol 4 (1) ◽  
pp. 168 ◽  
Author(s):  
Jun Zou ◽  
Xiao-Xi Huang ◽  
Guang-Wen Yin ◽  
Ye Ding ◽  
Xian-Yong Liu ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Live Vaccine ◽  
Vaccine Vector

scholarly journals Molecular Cloning and Characterization of Genes for Shigella sonnei Form I O Polysaccharide: Proposed Biosynthetic Pathway and Stable Expression in a Live Salmonella Vaccine Vector

2002 ◽  
Vol 70 (8) ◽  
pp. 4414-4423 ◽  
Author(s):  
De-Qi Xu ◽  
John O. Cisar ◽  
Nicholas Ambulos ◽  
Donald H. Burr ◽  
Dennis J. Kopecko
Keyword(s):  
Biosynthetic Pathway ◽  
Polyacrylamide Gel Electrophoresis ◽  
Surface Expression ◽  
Live Vaccine ◽  
Shigella Sonnei ◽  
Open Reading Frames ◽  
Vaccine Vector ◽  
Coding Region ◽  
Is Elements ◽  
The Stability

ABSTRACT The gene region for biosynthesis of Shigella sonnei form I O polysaccharide (O-Ps) and flanking sequences, totaling >18 kb, was characterized by deletion analysis to define a minimal construct for development of Salmonella-based live vaccine vector strains. Lipopolysaccharide (LPS) expression and DNA sequence studies of plasmid deletion derivatives indicated form I O-Ps expression from a 12.3-kb region containing a putative promoter and 10 contiguous open reading frames (ORFs), one of which is the transposase of IS630. A detailed biosynthetic pathway, consistent with the predicted functions of eight of the nine essential ORFs and the form I O-Ps structure, is proposed. Further sequencing identified partial IS elements (i.e., IS91 and IS630) and wzz upstream of the form I coding region and a fragment of aqpZ and additional full or partial IS elements (i.e., IS629, IS91, and IS911) downstream of this region. The stability of plasmid-based form I O-Ps expression was greater from low-copy vectors than from high-copy vectors and was enhanced by deletion of the downstream IS91 from plasmid inserts. Both core-linked (i.e., LPS) and non-core-linked (i.e., capsule-like) surface expression of form I O-Ps were detected by Western blotting and silver staining of polyacrylamide gel electrophoresis-separated Shigella and Escherichia coli extracts. However, salmonellae, which have a core that is chemically dissimilar to that of shigellae, expressed only non-core-linked surface-associated form I O-Ps. Finally, attenuated Salmonella enterica serovar Typhi live vaccine vector candidates, containing minimal-sized form I operon constructs, elicited immune protection in mice against virulent S. sonnei challenge, thereby supporting the promise of live, oral vaccines for the prevention of shigellosis.

Construction of recombinant Marek's disease virus type 1 (MDV1) expressing the Escherichia coli lacZ gene as a possible live vaccine vector: the US10 gene of MDV1 as a stable insertion site

Vaccine ◽  
1994 ◽  
Vol 12 (10) ◽  
pp. 953-957 ◽  
Author(s):  
Masashi Sakaguchi ◽  
Yumiko Hirayama ◽  
Hiroaki Maeda ◽  
Kazuo Matsuo ◽  
Michitaka Yamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Disease Virus ◽  
Virus Type ◽  
Insertion Site ◽  
Marek's Disease Virus ◽  
Live Vaccine ◽  
Vaccine Vector ◽  
Lacz Gene ◽  
Disease Virus Type

scholarly journals Establishment of Recombinant Eimeria acervulina Expressing Multi-Copies M2e Derived from Avian Influenza Virus H9N2

Vaccines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 791
Author(s):  
Sixin Zhang ◽  
Xinming Tang ◽  
Si Wang ◽  
Fangyun Shi ◽  
Chunhui Duan ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Eimeria Species ◽  
Live Vaccine ◽  
Vaccine Vector ◽  
Wing Vein ◽  
Transgenic Parasites

The potential of Eimeria parasites as live vaccine vectors has been reported with successful genetic manipulation on several species like E. tenella, E. mitis and E. necatrix. Among seven Eimeria species infecting chickens, E. acervulina is a highly prevalent, moderately pathogenic species. Thus, it is valuable for the study of transfection and for use as a potential as vaccine vector. In this study, a plasmid containing expression cassette with enhanced yellow fluorescent protein (EYFP), red fluorescent protein (RFP) and 12 copies of extracellular domain of H9N2 avian influenza virus M2 (M2e) protein was used for the transfection. Nucleofected sporozoites were inoculated into birds through wing vein. Recombinant E. acervulina oocysts with 0.1% EYFP+ and RFP+ populations were collected from the feces of the inoculated birds. The fluorescent rate of transgenic parasites reached over 95% after nine successive propagations with a pyrimethamine selection in vivo and fluorescent-activated cell sorting (FACS) of progeny oocysts. The expression of M2e in the transgenic parasites (EaM2e) was confirmed by Western blot and its cytoplasm localization in sporozoites was displayed by an indirect immunofluorescent assay (IFA). Meanwhile, we found that the fecundity of EaM2e was equivalent to that of wild type E. acervulina (EaWT). Taken together, the stable transfection of E. acervulina was successfully established. Future studies will focus on whether transgenic E. acervulina can serve as a live vaccine vector.

scholarly journals Cloning and manipulation of theCorynebacterium pseudotuberculosis recAgene for live vaccine vector development

1996 ◽  
Vol 142 (2-3) ◽  
pp. 139-145 ◽  
Author(s):  
Catherine A. Pogson ◽  
Cameron P. Simmons ◽  
Richard A. Strugnell ◽  
Adrian L.M. Hodgson
Keyword(s):  
Live Vaccine ◽  
Vaccine Vector
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