scholarly journals Molecular Detection and Characterization of Haemobartonella felis in Domestic Cats in Japan Employing Sequence-Specific Polymerase Chain Reaction (SS-PCR)

2003 ◽  
Vol 65 (10) ◽  
pp. 1111-1114 ◽  
Author(s):  
Masashi WATANABE ◽  
Masaharu HISASUE ◽  
Kazuko HASHIZAKI ◽  
Mitsuru FURUICHI ◽  
Munetsugu OGATA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Domestic Cats ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping

1998 ◽  
Vol 4 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Anna Gaszewska-Mastalarz ◽  
Marzena Bartoszewicz-Potyrała ◽  
Anna Przondo-Mordarska ◽  
Marian Mordarski ◽  
Jolanta Zakrzewska-Czerwińka
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Coagulase Negative Staphylococci ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals In VitroandIn VivoBiofilm Characterization of Methicillin-ResistantStaphylococcus aureusfrom Patients Associated with Pharyngitis Infection

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Shanmugaraj Gowrishankar ◽  
Arumugam Kamaladevi ◽  
Krishnaswamy Balamurugan ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Polymerase Chain Reaction ◽  
Congo Red ◽  
Molecular Detection ◽  
Microtitre Plate ◽  
Chain Reaction ◽  
Molecular Investigation ◽  
Polymerase Chain

The present investigation was deliberately aimed at evaluating the biofilm-forming ability of 63 clinical MRSA isolates recovered from pharyngitis patients through different phenotypic assays. The molecular detection of adhesion (icaA/icaD/icaB/icaC), adhesins (fnbA/fnbB,clfA, andcna), staphylococcal accessory regulator (sarA), andα-toxin (hla) genes was done by employing polymerase chain reaction (PCR). Out of 63 isolates, 49 (77.8%) were found slime positive by the Congo red agar (CRA) method and 44 (69.8%) as biofilm positive by the quantitative microtitre plate assays. The results of MATH assay showed that most of the test pathogens are hydrophilic in nature. The molecular investigation of biofilm-associated genes revealed that 84.13% (n=53) of isolates were found positive foricaADBCgenes. ThefnbAandfnbBgenes were present in 49 (77.8%) and 51 (81%) MRSA isolates, respectively. In addition, 58.7% (n=37), 73% (n=46), and 69.8% (n=44) of the isolates harboured theclfA,cna, andhlagenes, respectively. Further, nearly 81% (n=51) of the isolates were found positive for the genesarAand all theicanegative isolates were also negative for the gene. Furthermore, the results ofin vivoadherence assay unveiled the factual commonness in thein vitroadherence method.

scholarly journals Optimization of Polymerase Chain Reaction for Molecular Detection of Babesia Felis in Domestic Cats

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Muhammad Younus Khan ◽  
Haroon Akbar ◽  
Raheela Akhtar ◽  
Wasim Shehzad ◽  
Saher Islam ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Domestic Cats ◽  
Chain Reaction ◽  
Polymerase Chain

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

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