β-Galactosidase-catalyzed Synthesis of 1, 2-Hexanediol Galactoside and its Purification using Ethyl Acetate Extraction followed by Silica Gel Chromatography

2016 ◽  
Vol 33 (3) ◽  
pp. 498-506
Author(s):  
Yi-Ok Kim ◽  
Kyung-Hwan Jung
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Gel Chromatography ◽  
Silica Gel Chromatography

Gas-Liquid Chromatographic Determination of Captan and Two Metabolites in Milk and Meat

1977 ◽  
Vol 60 (3) ◽  
pp. 679-681
Author(s):  
John H Onley
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Chromatographic Determination ◽  
Gel Chromatography ◽  
Milk Samples ◽  
Liquid Chromatographic Determination ◽  
Meat Samples ◽  
Silica Gel Chromatography ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic (GLC) method has been developed for the determination of captan (N-trichloromethylthio-4-cyclohexene-1, 2-dicarboximide) and 2 metabolites, tetrahydrophthalimide (THPI) and tetrahydrophthalamic acid (THPMA), in milk and meat. The sample is extracted with ethyl acetate and is cleaned up by acetonitrile partition and silica gel chromatography where captan, THPI, and THPMA are separated. Captan is directly determined by GLC. THPI and THPMA are separately derivatized in an acetone solution of penta fluorobenzyl bromide. The resultant derivatives are purified separately on an Al2O3, column and quantitated by GLC, using an electron capture detector. Recoveries from milk samples fortified at 0.02–10 ppm ranged from 71 to 102%; recoveries from meat samples fortified at 0.04–10 ppm ranged from 75 to 99%.

scholarly journals CAAC Boranes. Synthesis and characterization of cyclic (alkyl) (amino) carbene borane complexes from BF3 and BH3

2010 ◽  
Vol 6 ◽  
pp. 709-712 ◽  
Author(s):  
Julien Monot ◽  
Louis Fensterbank ◽  
Max Malacria ◽  
Emmanuel Lacôte ◽  
Steven J Geib ◽  
...  
Keyword(s):  
Silica Gel ◽  
Synthesis And Characterization ◽  
Gel Chromatography ◽  
Nmr Analysis ◽  
X Ray ◽  
X Ray Crystallography ◽  
In Situ Formation ◽  
Silica Gel Chromatography

In situ formation of two cyclic (alkyl) (amino) carbenes (CAACs) followed by addition of BF3•Et2O provided the first two examples of CAAC–BF3 complexes: 1-(2,6-diisopropylphenyl)-3,5,5-trimethyl-3-phenylpyrrolidin-2-ylidene trifluoroborane, and 2-(2,6-diisopropylphenyl)-3,3-dimethyl-2-azaspiro[4.5]decan-1-ylidene trifluoroborane. These CAAC–BF3 complexes are robust compounds that are stable to ambient laboratory conditions and silica gel chromatography. They were characterized by spectroscopy and X-ray crystallography. In contrast, a CAAC complex with borane (BH3) was readily formed in situ according to 1H and 11B NMR analysis, but did not survive the workup conditions. These results set the stage for further studies of the chemistry of CAAC boranes.

Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reversed-Phase Silica Gel Chromatography

2016 ◽  
pp. 87-93 ◽  
Author(s):  
Anna C. A. Yendo ◽  
Fernanda de Costa ◽  
Carla Kauffmann ◽  
Juliane D. Fleck ◽  
Grace Gosmann ◽  
...  
Keyword(s):  
Silica Gel ◽  
Reversed Phase ◽  
Gel Chromatography ◽  
Saponin Fraction ◽  
Silica Gel Chromatography

Purification of deoxynivalenol (vomitoxin) by water-saturated silica gel chromatography

1985 ◽  
Vol 33 (4) ◽  
pp. 745-748 ◽  
Author(s):  
Mary F. Witt ◽  
L. Patrick Hart ◽  
James J. Pestka
Keyword(s):  
Silica Gel ◽  
Gel Chromatography ◽  
Silica Gel Chromatography ◽  
Water Saturated

scholarly journals Determination of Phospholipid Fractions in Red Blood Cells in Retarded Physically Handicapped Children

1973 ◽  
Vol 10 (1-6) ◽  
pp. 23-27 ◽  
Author(s):  
Fay M. John ◽  
G. C. Forrest
Keyword(s):  
Red Blood Cells ◽  
Silica Gel ◽  
Blood Cells ◽  
Red Cells ◽  
Gel Chromatography ◽  
Handicapped Children ◽  
Silica Gel Chromatography ◽  
Physically Handicapped

Phospholipids from the red cells of 85 children, both normal and belonging to various clinical groups, have been extracted and subjected to silica gel chromatography. The several phospholipids have been quantitated. The results have not proved to be useful in diagnosis and hence do not confirm earlier reports. The problems associated with analysis of phospholipids by this type of method are discussed.

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