RAPD-ANALYSIS OF CYCLAMEN SPP. GENOME POLYMORPHISM

Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

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Short Communication Biochemical and Molecular Genetic Studies on Some Cyanobacterial Isolates

Biological Sciences - PJSIR ◽

10.52763/pjsir.biol.sci.54.3.2011.172.174 ◽

2011 ◽

Vol 54 (3) ◽

pp. 172-174

Author(s):

Ehab Abdel-Razik Kamel ◽

Samier Abdel-Aziz Ebrahim ◽

Rabea Abdel- Tawab Thabet ◽

Engy Mohamed Rabea El-Shal

Keyword(s):

Rapd Markers ◽

Molecular Genetic ◽

Principal Component ◽

Genetic Studies ◽

Molecular Fingerprints ◽

Isolation And Purification ◽

Rapd Pcr ◽

Sds Page ◽

Genetic Techniques ◽

Polymorphic Bands

In the present study, the isolation and purification of a set of Cyanobacteria strains belonging to genus Oscillatoria was undertaken, followed by the analyses of phylogenetic relationships using different biochemical and molecular genetic techniques (SDS-PAGE and RAPD-PCR). A total of 45 protein bands were observed within the studied Oscillatoria isolates by SDS-PAGE (only three unique bands, eight monomorphic bands and 37 polymorphic bands). On the other hand, extracted DNA from isolates was used to identify the molecular fingerprints. A sum of 94 polymorphic bands was generated by these primers in the Ocsillatoria genotypes under study. A total of 20 unique bands were identified out of the polymorphic ones. These unique bands were used to discriminate among the studied Ocsillatoria isolates. Most isolates of Ocsillatoria genotypes were discriminated by one or more unique bands. Numerical taxonomic using 45 protein attributes of 19 isolates and RAPD markers on five isolates. Two methods - Clustering (UPGMA) and Principal Component Analysis (PCA) were used for these analyses. The similarities and clusters produced between the studied isolates were discussed.

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Prevalence of nontuberculous mycobacteria in Gomel region

Health and Ecology Issues ◽

10.51523/2708-6011.2020-17-2-11 ◽

2020 ◽

pp. 78-84

Author(s):

I. V. Bujnevich ◽

D. Yu. Ruzanov ◽

L. V. Zolotuhina ◽

V. N. Bondarenko ◽

V. I. Majseenko ◽

...

Keyword(s):

Species Diversity ◽

Nontuberculous Mycobacteria ◽

Molecular Genetic ◽

Genetic Studies ◽

The Third ◽

Common Causes

Objective: to assess the species diversity and frequency of detection of nontuberculous mycobacteria (NTM) in patients of antituberculous organizations in Gomel region. Material and methods. 1057 isolates of mycobacteria were analyzed. To identify the species of the mycobacteria, phenotypic and molecular genetic studies of the isolates were performed. Results. A total of 13 NTM types have been identified. The most common were M. avium and M. intracellulare (MAC complex) - 63.7 % (60.79-66.67), followed by M. gordonae and M. fortuitum (7.66 %; 6.13-9.43 and 7.57 %; 6.05-9.33, respectively). Multiple isolation of NTMs of one species (from 2 or more samples) was observed in 190 patients, which amounted to 37.4 % of all the patients. In 75.3 % of the cases, MAC was obtained (χ2 = 97.01; p <0.001). The second place in terms of etiological significance was occupied by M. xenopi (6.43 %), the third one - by M. gordonae (5.3 %). Conclusion. Out of 13 identified NTMs, the most common causes of the development of mycobacteriosis were MAС complex, M. xenopi , M. gordonae .

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Using RAPD-markers in studying molecular genetic polymorphism in the genus Miscanthus species

Agrobìologìâ ◽

10.33245/2310-9270-2019-146-1-6-12 ◽

2019 ◽

pp. 6-12

Author(s):

A. Bab'jazh ◽

O. Cherednychok ◽

N. Hryhorenko

Keyword(s):

Genetic Polymorphism ◽

Molecular Genetic ◽

Bioenergy Crops ◽

Genetic Method ◽

Plant Groups ◽

Rapd Pcr ◽

Model Sample ◽

Different Populations ◽

The Difference ◽

High Level

Aim. The study aims to investigate molecular genetic polymorphism in the representatives of different populations and bioenergy crops plant groups of the genus Miscanthus of the collection of the Institute of Bioenergy Crops and Sugar Beet of the NAAS of Ukraine as well as to search and select the molecular markers to differentiate them using RAPD PCR methods. Methods. The studies used the molecular genetic method for detecting polymorphism by analyzing the lengths of amplification fragments and the method of electrophoretic distribution of amplification products in agarose gel. Results. In the course of amplification with 7 RAPD primers, 28 loci were obtained, of which 23 were polymorphic. The polymorphism degree averaged 65 %. The index of polymorphism locus ranged from 0.17 to 0. 56. There were identified six alleles by marker P822 -with sizes ranging from 230 to 613 bp. P820 primer amplified two loci of 311 and 482 bp, three alleles sized from 219 to 530 bp were identified by P816 and P817 markers. The spectra of the amplicons obtained using the above-mentioned primers make it possible to differentiate representatives of the Miscanthus genus of different species, since the difference in the number of loci for each species representative is established. A unique allele with the frequency of 0.35 and the size of 605 bp was obtained using the RAPD2 primer. Conclusions. According to the results it was found that the use of primers P816 and RAPD2 allowed to separate genotypes of M. sacchariflorus, M. gigantheus, M. sinensis. A large proportion of polymorphic loci confirm the population composition of the model sample which resulted in high level of polymorphism. Key words: genus Miscanthus, RAPD primers, PCR, polymorphism, allele frequencies.

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Inheritance of random amplified polymorphic DNA markers in cassava (Manihot esculenta Crantz)

Genome ◽

10.1139/g96-130 ◽

1996 ◽

Vol 39 (5) ◽

pp. 1039-1043 ◽

Cited By ~ 9

Author(s):

Rocio Gomez ◽

Fernando Angel ◽

Merideth W. Bonierbale ◽

Fernando Rodriguez ◽

Joseph Tohme ◽

...

Keyword(s):

Single Dose ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Manihot Esculenta Crantz ◽

Genetic Studies ◽

Genetic Segregation ◽

Molecular Genetic Map ◽

History Of ◽

Hybrid Clones

The informativeness and inheritance of randomly amplified polymorphic DNA (RAPD) markers were investigated in an intraspecific F1 progeny derived from two heterozygous parents. The analysis confirmed the utility of RAPD markers for comparing candidate parents for the development of a molecular genetic map, and provided numerous markers for linkage analysis in a crop with a very limited history of classical or molecular genetic studies. Six potential parental lines (themselves F1 hybrid clones) showed between 1.82 and 0.62 segregating bands per primer in three hybrid families. Forty-three percent (309) of 722 primers produced polymorphic products in the most informative of these three crosses, revealing 328 single-dose (SD) markers segregating 1:1 for presence/absence in a progeny of 90 individuals. A second class of informative markers were those present in both parents but segregating in the progeny. Fifty-seven or 67% of the monomorphic but segregating markers exhibited the 3:1 ratio expected for SD dominant markers in a cross between heterozygotes. Linkage groups were constructed from the segregation of SD RAPD markers originating in the female (TMS 30572) and the male (CM2177-2) parent. Key words : RAPDs, molecular markers, genetic segregation, Manihot, single-dose markers.

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THE SYNTAXONOMIC AND SPECIES DIVERSITY OF THE CLASS FESTUCO-BROMETEA BR.-BL. EX KLIKA & HADAČ 1944 IN THE AREA OF PREČKO POLJE

Radovi Šumarskog fakulteta Univerziteta u Sarajevu ◽

10.54652/rsf.2019.v49.i2.33 ◽

2021 ◽

Vol 49 (2) ◽

pp. 27-32

Author(s):

Sabina Trakić ◽

Emina Sarač-Mehić ◽

Velida Durmić ◽

Sedik Velić

Keyword(s):

Species Diversity ◽

Plant Species ◽

Biological Diversity ◽

Conservation Status ◽

Natura 2000 ◽

Orchid Species ◽

Natura 2000 Site ◽

Mesobromion Erecti ◽

High Degree ◽

Rare Endemic

UDK: 581.55(497.6) The ecosystems of the class Festuco-Brometea in Bosnia and Herzegovina play an important role in the overall biological diversity. They are distinguished by a large number of vegetation units, as well as by significant number of rare, endemic or relict plant species. The study area, Prečko polje, covers 1.27 at 1100 m a.s.l., and takes place in the heart of mountain complex Treskavica-Visočica-Bjelašnica. We analised thermophylous meadows of Prečko polje after method of phytocoenological relevé. The class Festuco-Brometea in study area is being differentiated into the alliances Xerobromion erecti (with suballiance Fumano-Scabiosenion leucophyllae) and Mesobromion erecti (with suballiance Eu-Mesobromenion). In general, thermophylous meadows are characterized by high degree of species diversity. Moreover, within thermophylous meadows of Prečko polje we have identified nine species which are considered to have certain conservation status, according to the national and/or international legislation. Our study has shown that investigated area should be considered as potential Natura 2000 site for it is inhabited by a significant number of rare and/or endangered orchid species.

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