Short Communication Biochemical and Molecular Genetic Studies on Some Cyanobacterial Isolates

In the present study, the isolation and purification of a set of Cyanobacteria strains belonging to genus Oscillatoria was undertaken, followed by the analyses of phylogenetic relationships using different biochemical and molecular genetic techniques (SDS-PAGE and RAPD-PCR). A total of 45 protein bands were observed within the studied Oscillatoria isolates by SDS-PAGE (only three unique bands, eight monomorphic bands and 37 polymorphic bands). On the other hand, extracted DNA from isolates was used to identify the molecular fingerprints. A sum of 94 polymorphic bands was generated by these primers in the Ocsillatoria genotypes under study. A total of 20 unique bands were identified out of the polymorphic ones. These unique bands were used to discriminate among the studied Ocsillatoria isolates. Most isolates of Ocsillatoria genotypes were discriminated by one or more unique bands. Numerical taxonomic using 45 protein attributes of 19 isolates and RAPD markers on five isolates. Two methods - Clustering (UPGMA) and Principal Component Analysis (PCA) were used for these analyses. The similarities and clusters produced between the studied isolates were discussed.

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RAPD-ANALYSIS OF CYCLAMEN SPP. GENOME POLYMORPHISM

CBU International Conference Proceedings ◽

10.12955/cbup.v7.1483 ◽

2019 ◽

Vol 7 ◽

pp. 949-953

Author(s):

Elza Makaradze ◽

Galina Meparishvili ◽

Natela Varshanidze ◽

Inga Diasamidze ◽

Ketevan Dolidze ◽

...

Keyword(s):

Genetic Polymorphism ◽

Species Diversity ◽

Rapd Markers ◽

Biological Diversity ◽

Molecular Genetic ◽

Wild Plants ◽

Large Species ◽

Genetic Studies ◽

Rapd Pcr ◽

Rare Endemic

Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

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Understanding the roles of genome and envirome: methods in genetic epidemiology

The British Journal of Psychiatry ◽

10.1192/bjp.178.40.s12 ◽

2001 ◽

Vol 178 (S40) ◽

pp. s12-s17 ◽

Cited By ~ 19

Author(s):

Jenae M. Neiderhiser

Keyword(s):

Genetic Epidemiology ◽

Early Stage ◽

Molecular Genetic ◽

Basic Knowledge ◽

Careful Examination ◽

Specific Gene ◽

Environment Interaction ◽

Genetic Studies ◽

Genetic Techniques ◽

Complex Relationships

BackgroundIn order to understand studies of psychiatric epidemiology focusing on the ‘genome’ and ‘envirome’, basic knowledge of the logic and methods is necessary.AimsTo provide a review of typical methods used in genetic epidemiology.MethodReviews of the research designs usually employed in quantitative and molecular genetic studies. Genotype-environment correlation and interaction are also discussed.ResultsQuantitative genetic studies indicate that genetic influences are important for both psychiatric disorders and behavioural traits. Specific gene loci can be tested for associations with both psychiatric risk and behavioural traits by means of molecular genetic techniques. There has been little examination of genotype-environment correlation and interaction, although the few reports that have appeared suggest that these complex relationships are important.ConclusionsAdvances in quantitative and molecular genetics now permit more careful examination of genotype-environment interaction and correlation. Studies combining molecular genetic strategies with measurement of the environment are still at an early stage, however, and their results must be awaited.

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When Genetics Gets under Your Skin

Journal of Cutaneous Medicine and Surgery ◽

10.1177/120347549600100208 ◽

1996 ◽

Vol 1 (2) ◽

pp. 97-100 ◽

Cited By ~ 6

Author(s):

Sherri J. Bale

Keyword(s):

Risk Factors ◽

Skin Diseases ◽

Association Studies ◽

Molecular Genetic ◽

Underlying Mechanism ◽

Statistical Techniques ◽

Disease Genes ◽

Genetic Studies ◽

Genetic Techniques ◽

Skin Biology

Background: Only recently has the advent of the use of modern statistical and molecular genetic techniques begun to increase our understanding of the study of dermatology and skin biology. Objective: This paper will briefly outline several statistical techniques that are used in genetic studies of skin disease by reviewing these techniques, the types of questions that can be answered using them, and issues that should be considered in evaluating and interpreting papers that use them. Methods: A discussion of association studies, segregation analyses, and linkage analyses with respect to skin diseases is presented. Results: Association studies can be used to identify both genetic and environmental risk factors for disease. Segregation analyses are used to identify the underlying mechanism for disease aggregation in families. Linkage analysis is used to map disease genes to chromosomes. Conclusion: Dermatologists should be familiar with the types of genetic questions that can be answered with each technique, and should remain aware of the limitations in interpretation.

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Inheritance of random amplified polymorphic DNA markers in cassava (Manihot esculenta Crantz)

Genome ◽

10.1139/g96-130 ◽

1996 ◽

Vol 39 (5) ◽

pp. 1039-1043 ◽

Cited By ~ 9

Author(s):

Rocio Gomez ◽

Fernando Angel ◽

Merideth W. Bonierbale ◽

Fernando Rodriguez ◽

Joseph Tohme ◽

...

Keyword(s):

Single Dose ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Manihot Esculenta Crantz ◽

Genetic Studies ◽

Genetic Segregation ◽

Molecular Genetic Map ◽

History Of ◽

Hybrid Clones

The informativeness and inheritance of randomly amplified polymorphic DNA (RAPD) markers were investigated in an intraspecific F1 progeny derived from two heterozygous parents. The analysis confirmed the utility of RAPD markers for comparing candidate parents for the development of a molecular genetic map, and provided numerous markers for linkage analysis in a crop with a very limited history of classical or molecular genetic studies. Six potential parental lines (themselves F1 hybrid clones) showed between 1.82 and 0.62 segregating bands per primer in three hybrid families. Forty-three percent (309) of 722 primers produced polymorphic products in the most informative of these three crosses, revealing 328 single-dose (SD) markers segregating 1:1 for presence/absence in a progeny of 90 individuals. A second class of informative markers were those present in both parents but segregating in the progeny. Fifty-seven or 67% of the monomorphic but segregating markers exhibited the 3:1 ratio expected for SD dominant markers in a cross between heterozygotes. Linkage groups were constructed from the segregation of SD RAPD markers originating in the female (TMS 30572) and the male (CM2177-2) parent. Key words : RAPDs, molecular markers, genetic segregation, Manihot, single-dose markers.

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Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Folia Forestalia Polonica ◽

10.2478/ffp-2019-0025 ◽

2019 ◽

Vol 61 (4) ◽

pp. 255-266

Author(s):

◽

Rini Hafzari ◽

Tia Setiawati ◽

Budi Irawan ◽

Joko Kusmoro

Keyword(s):

Genetic Diversity ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Similarity Coefficient ◽

Bamboo Species ◽

Base Pairs ◽

Natural Genetic Variation ◽

Molecular Genetic Diversity ◽

Polymorphic Bands

Abstract Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

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RAPD-PCR identification of Verticillium dahliae isolates with differential pathogenicity on cotton

Australian Journal of Agricultural Research ◽

10.1071/ar9960681 ◽

1996 ◽

Vol 47 (5) ◽

pp. 681 ◽

Cited By ~ 16

Author(s):

JR Ramsay ◽

DS Multani ◽

BR Lyon

Keyword(s):

Verticillium Dahliae ◽

Dna Sequences ◽

Molecular Genetic ◽

Economic Losses ◽

Pcr Analysis ◽

In Planta ◽

Rapd Pcr ◽

Fungal Populations ◽

Genetic Techniques

Strains of the fungal wilt pathogen Verticillium dahliae cause considerable economic losses in field crops throughout the world. We have investigated the use of molecular genetic techniques for the identification and classification of strains infecting cotton plants (Gossypium spp.) in the major production regions of Australia. The amplification, sequencing, and restriction digestion of specific ribosomal DNA sequences proved to be diagnostic for the genus/species, but was incapable of differentiating between individual isolates with quite diverse morphologies or origins. RAPD-PCR analysis using a subset of 13 informative decamer primers, however, did reveal significant differences between the isolates and enabled the genetic similarity of the V. dahliae strains to be estimated. RAPD-PCR fingerprints were shown to be reproducible following in planta culture of V. dahliae isolates and could be a valuable tool for the identification and epidemiological study of fungal populations. Although fungal isolates were found to elicit a range of reactions during pathogenicity testing on 5 cotton cultivars, comparison of the RAPD-PCR banding patterns did not suggest a strong correlation between molecular genetic and virulence characteristics.

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