Inheritance of random amplified polymorphic DNA markers in cassava (Manihot esculenta Crantz)

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1039-1043 ◽  
Author(s):  
Rocio Gomez ◽  
Fernando Angel ◽  
Merideth W. Bonierbale ◽  
Fernando Rodriguez ◽  
Joseph Tohme ◽  
...  
Keyword(s):  
Single Dose ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Manihot Esculenta Crantz ◽  
Genetic Studies ◽  
Genetic Segregation ◽  
Molecular Genetic Map ◽  
History Of ◽  
Hybrid Clones

The informativeness and inheritance of randomly amplified polymorphic DNA (RAPD) markers were investigated in an intraspecific F1 progeny derived from two heterozygous parents. The analysis confirmed the utility of RAPD markers for comparing candidate parents for the development of a molecular genetic map, and provided numerous markers for linkage analysis in a crop with a very limited history of classical or molecular genetic studies. Six potential parental lines (themselves F1 hybrid clones) showed between 1.82 and 0.62 segregating bands per primer in three hybrid families. Forty-three percent (309) of 722 primers produced polymorphic products in the most informative of these three crosses, revealing 328 single-dose (SD) markers segregating 1:1 for presence/absence in a progeny of 90 individuals. A second class of informative markers were those present in both parents but segregating in the progeny. Fifty-seven or 67% of the monomorphic but segregating markers exhibited the 3:1 ratio expected for SD dominant markers in a cross between heterozygotes. Linkage groups were constructed from the segregation of SD RAPD markers originating in the female (TMS 30572) and the male (CM2177-2) parent. Key words : RAPDs, molecular markers, genetic segregation, Manihot, single-dose markers.

scholarly journals Molecular genetic verification of isolated mineralocorticoid deficiency due to aldosterone synthase deficiency

2009 ◽  
Vol 55 (1) ◽  
pp. 28-30
Author(s):  
N Yu Kalinchenko ◽  
N A Zubkova ◽  
A N Tyulpakov
Keyword(s):  
Autosomal Recessive ◽  
Molecular Genetic ◽  
Autosomal Recessive Disorder ◽  
Adrenal Hyperplasia ◽  
Genetic Studies ◽  
Aldosterone Synthase ◽  
Salt Wasting ◽  
History Of ◽  
17 Hydroxyprogesterone ◽  
Aldosterone Synthase Deficiency

Isolated mineralocorticoid deficiency is a rare hereditary autosomal recessive disorder that is characterized by salt wasting and that has the severest manifestations in infants. This paper is the first in the Russian literature to describe cases of isolated aldosterone deficiency. In both cases, the patients were monitored and treated for misdiagnosed congenital adrenal hyperplasia; however, the permanently low level of 17-hydroxyprogesterone could put in doubt the diagnosis and suspect isolated mineralocorticoid deficiency, by keeping in mind a history of salt wasting. By using the presented cases as an example, the authors give an algorithm for the examination and differential diagnosis of this condition and other diseases that have the similar clinical picture. Aldosterone synthase deficiency in patients was verified by molecular genetic studies - there were mutations in the CYP112 gene.

scholarly journals RAPD-ANALYSIS OF CYCLAMEN SPP. GENOME POLYMORPHISM

2019 ◽  
Vol 7 ◽  
pp. 949-953
Author(s):  
Elza Makaradze ◽  
Galina Meparishvili ◽  
Natela Varshanidze ◽  
Inga Diasamidze ◽  
Ketevan Dolidze ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Species Diversity ◽  
Rapd Markers ◽  
Biological Diversity ◽  
Molecular Genetic ◽  
Wild Plants ◽  
Large Species ◽  
Genetic Studies ◽  
Rapd Pcr ◽  
Rare Endemic

Wild plants form the basis of biological resources both for Georgia and the whole world. A strategic task of any country is to preserve the biological diversity of plants. In the territory of Ajara, a large species diversity of plants grows, among which there are rare, endemic and relict plants. In particular, Cyclamen adzharicum. Modern systematics of wild plants in Georgia is based on classical methods of botany. In this regard, it is relevant to conduct genetic studies of species diversity and genetic polymorphism of species and populations using molecular genetic markers, in particular RAPD-PCR methods. The purpose of this study was to identify genetic polymorphism in Cyclamen L species using RAPD methods. As a result of the conducted research the 65 RAPD-markers in length from 150 to 1500 BP have been revealed. The number of the amplified fragments DNA varied depending on the primer from 6 (OPA-2) to 11 (OPB-4). The results of grouping Cyclamen adzharicum and C. coum samples allowed two clusters to be identified. In the first cluster were samples of three populations Cyclamen adzharicum and showed a low stubble in the intra-species variability. Cyclamen coum was attributed to the second cluster. The used primers gave the opportunity to identify polymorphism between the tested types of cyclamen.

Molecular Diversity Analysis of Lentil (Lens culinaris Medik.) through RAPD Markers

2012 ◽  
Vol 22 (1) ◽  
pp. 51-58 ◽  
Author(s):  
M.E. Hoque ◽  
M.M. Hasan
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Group Method ◽  
Diversity Analysis ◽  
Pair Group ◽  
Molecular Genetic Diversity

Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular genetic diversity analysis among six BARI released lentil varieties viz. BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. PCR amplified products were visualized on 1.0% agarose gel and the band for each primer were scored. Ten RAPD markers were used in this study. Out of them 7 primers showed amplification of 53 DNA fragments with 60.37% of them being polymorphic. The highest number of polymorphic loci was noticed in the variety BARI masur-3. The same variety also showed maximum Nei’s gene diversity value (0.0552). The highest Nei’s genetic distance (0.5002) was observed in BARI masur-1 vs. BARI masur-5 whereas, the lowest genetic distance (0.0692) was found in BARI masur-1 vs. BARI masur-2. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s genetic distance grouped the six cultivars into two main clusters. BARI masur-1, BARI masur-2 and BARI masur-3 were in cluster I and BARI masur-4, BARI masur-5 and BARI masur-6 were in cluster II. The cultivar BARI masur-4 was closest to the cultivar BARI masur-6 with the lowest genetic distance (0.0972) and the highest genetic distance (0.5002) was found between BARI masur-1 and BARI masur-5. The RAPD markers were found to be useful in molecular characterization of lentil varieties which could be utilized by the breeders for the improvement of lentil cultivars. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11260 Plant Tissue Cult. & Biotech. 22(1): 51-58, 2012 (June)

Primary Pure Angiosarcoma of the Testis: A Case Report

2014 ◽  
Vol 1 ◽  
Author(s):  
Samay Jain ◽  
Richard Cantley ◽  
Justus Philip
Keyword(s):  
Germ Cell ◽  
Molecular Genetic ◽  
Malignant Neoplasm ◽  
Cell Precursor ◽  
Cell Component ◽  
Genetic Studies ◽  
Spindle Cell Neoplasm ◽  
Primary Angiosarcoma ◽  
History Of ◽  
Vascular Channels

Angiosarcoma is a rare and aggressive, malignant neoplasm of endothelial-cell origin.   A primary angiosarcoma originating in the testicle is extremely rare, with only five previous cases reported in the current literature. We report a case of primary, pure angiosarcoma of the testis in a 63-year-old patient with no history of previous chemotherapy or radiation therapy. By histology, the tumor was a high-grade spindle cell neoplasm, arranged in sheets and poorly-formed vascular channels. The tumor cells were positive for vascular markers (CD31, CD34) by immunohistochemical staining. No evidence of a germ cell component was seen by morphology, immunohistochemistry, or molecular genetic studies. This finding is unique in that it is one of only three reported cases of primary angiosarcomas of the testicle without a germ cell precursor or component.

scholarly journals Molecular characterization of onion (Allium cepa) using RAPD markers

1970 ◽  
Vol 35 (2) ◽  
pp. 313-322 ◽  
Author(s):  
M Maniruzzaman ◽  
ME Haque ◽  
MM Haque ◽  
MA Sayem ◽  
M Al-Amin
Keyword(s):  
Allium Cepa ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Group Method ◽  
Genetic Studies ◽  
Pair Group ◽  
Genetic Level ◽  
Polymerase Chain

A polymerase chain reaction (PCR) based approach, namely random amplified polymorphic DNA (RAPD) analysis was applied to l0 varieties of onion (Allium cepa) in order to assess the degree of polymorphism within the genes and to investigate if this approach was suitable for genetic studies of onion. For this study, ten cultivars of onion were evaluated for variability using a set of 15 random l0-mer primers. The polymorphisms in PCR amplification products were subjected to the unweighed pair group method for arithmetic averages (UPGMA) and plotted in a phenogram. The dendogram constructed from the similarity data showed that all the cultivars analyzed were related. Among them, 12 of the primers revealed scorable (168 bands) polymorphisms between cultivars of A. cepa and the rest did not show polymorphism in their genetic level. In this study, it was found that Bermis and India-2 were more dissimilar and on the other hand, Faridpuri and Bhati were the most similar in their genetic level. Keywords: RAPD; onion; genetic diversity; polymorphism. DOI: 10.3329/bjar.v35i2.5894Bangladesh J. Agril. Res. 35(2) : 313-322, June 2010

scholarly journals Study genetic diversity in maize inbreds and improved hybrid vigor in hybrids produce to determine commercial hybrids in Iraq

2015 ◽  
Vol 5 (3) ◽  
pp. 728-731
Author(s):  
Ziyad A. Abed
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Field Experiments ◽  
Random Amplified Polymorphic Dna ◽  
Hybrid Vigor ◽  
Breeding Programs ◽  
Genetic Studies ◽  
Maize Inbreds ◽  
High Level

 A field experiments was conducted in greenhouse to determinate the genetic diversity among 7 genotypes from maize(4 inbreds and 3hybrids) by using molecular markers with Random Amplified polymorphic DNA(RAPD),that shown high level of polymorphism among genotypes of maize ,where the percentage of polymorphism ranged from(66%) and (83.33%) the highest number of polymorphism band (16) and size fragment ranged between (3800 bp) with the primer ( Bnlg 1185 ) and the lowest 180 with the primer( Bnlg 1464).The genetic distance value ranged between (0.3451) and (0.6534) ,where the lowest genic distance between (k1 and k2),while the highest genetic distance between(k4) and (k3xk4).In this study RAPD markers were shown to be powerful to detect genetic diversity and provided us high polymorphism values within genotypes of maize ,also we can conclude for useful those primers for genetic studies in plant breeding programs for developing synthetic cultivars or improved inbreds of maize. 

scholarly journals Genetic Diversity and Discrimination of Chimonanthus praecox (L.) Link Germplasm Using ISSR and RAPD Markers

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1144-1148 ◽  
Author(s):  
Kai-Ge Zhao ◽  
Ming-Qin Zhou ◽  
Long-Qing Chen ◽  
Donglin Zhang ◽  
Gituru Wahiti Robert
Keyword(s):  
Genetic Relationship ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficients ◽  
Flower Plant ◽  
History Of ◽  
The Difference ◽  
Relationship Of ◽  
First Time ◽  
Chimonanthus Praecox

Chimonanthus praecox (wintersweet) is endemic to China. It has been cultivated there for more than 1000 years as a garden, potted, and cut-flower plant. Many cultivars have been developed during its long history of cultivation, and recently many germplasms were collected in Wuhan and Nanjing, China. The identification and genetic relationship of these resources were studied based mainly on morphological traits. In the current study, intersimple sequence repeat markers (ISSR) and random amplified polymorphic DNA markers (RAPD) were used for the first time to investigate 72 wintersweet clones from the two regions. Eleven ISSR primers amplified 115 bands, 90 (78.26%) of which were polymorphic. Nineteen RAPD primers amplified 165 bands, 105 (63.63%) of which were polymorphic. Either ISSR or RAPD markers were sufficient to distinguish all the clones surveyed. A Dendrogram based on Jaccard's similarity coefficients indicated that the distribution pattern of the 72 clones was coherent with their geographical origins. Most of the genetic variation (85.68% with ISSR data; 86.75% with RAPD data) occurred among clones within each region. However, the difference between Wuhan and Nanjing groups is statistically significant (ΦST = 0.143, P < 0.001, with ISSR data; ΦST = 0.132, P < 0.001, with RAPD data). Morphological variation and classification of wintersweet cultivars were also discussed compared with the genetic relationship based on ISSR and RAPD markers. This is the first report of the partitioning of genetic variability within and between different cultivated wintersweet regions, and it provides useful baseline data for optimizing sampling strategies in breeding. These results are important for future genetic improvement, identification, and conservation of Chimonanthus praecox germplasm.

Strict maternal inheritance of RAPD fingerprints confirms apomixis in cassava (Manihot esculenta Crantz)

1996 ◽  
Vol 76 (2) ◽  
pp. 379-382 ◽  
Author(s):  
Dario Grattapaglia ◽  
Cynthia Costa e Silva ◽  
Nagib M. A. Nassar
Keyword(s):  
Key Words ◽  
Interspecific Hybrid ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Maternal Inheritance ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Evidence ◽  
Manihot Esculenta Crantz ◽  
Rapd Fingerprints

Undisputed molecular evidence for apomixis in cassava is presented. Based on genome sampling with 130 RAPD markers we confirmed apomixis for one offspring of a cassava clone derived from an interspecific hybrid. The probability that complete uniformity in RAPD fingerprints between mother and offspring occurred by chance was < 10−5. Key words: Apomixis, cassava, Manihot esculenta, random amplified polymorphic DNA

scholarly journals A molecular genetic map of cassava (Manihot esculenta Crantz)

1997 ◽  
Vol 95 (3) ◽  
pp. 431-441 ◽  
Author(s):  
M. Fregene ◽  
F. Angel ◽  
R. Gomez ◽  
F. Rodriguez ◽  
P. Chavarriaga ◽  
...  
Keyword(s):  
Genetic Map ◽  
Manihot Esculenta ◽  
Molecular Genetic ◽  
Manihot Esculenta Crantz ◽  
Molecular Genetic Map

scholarly journals Molecular genetic studies of Chilean avifauna: an overview about current progress

2019 ◽  
Vol 14 (4) ◽  
pp. 459-477
Author(s):  
Nelson Colihueque ◽  
Alberto Gantz
Keyword(s):  
Molecular Genetic ◽  
Research Field ◽  
Bibliographic Databases ◽  
Genetic Studies ◽  
Origin And Evolution ◽  
Research Areas ◽  
Or Groups ◽  
D Loop ◽  
History Of ◽  
Active Research

The biodiversity of Chilean avifauna has been shaped by geological events, geographical factors and extreme climatic regimens. Molecular genetic studies developed to date have begun to provide valuable information to elucidate the effect of these factors on the evolutionary history of Chilean birds. We conducted a search in two bibliographic databases (PubMed and Scopus) over the period between 1998 and 2018 to identify the number and class of articles published in this research field. The quantification of specific research categories was also performed to assess progress in different molecular genetic issues of Chilean birds. Representative studies of the more active research areas: population structure, phylogeography, systematics and taxonomy and genetic diversity, were briefly commented on. Studies on genetic divergence analysis of cryptic species and the underlying genetics at molecular level of adaptive phenotypes in populations, were very scarce. Several types of mitochondrial and nuclear markers have been used, with COI plus D-loop and microsatellite loci, respectively, being the most widely-used markers. However, high-throughput analyses based on complete mitochondrial genomes have also been published. More than one hundred species have been analyzed; the most studied groups being penguins, followed by New World swallows. Although, to date, molecular genetic studies of Chilean birds still require further analysis, the studies available on some species or groups have provided important data to improve our understanding of the origin and evolution of this avifauna.

Sign in / Sign up

Export Citation Format

Share Document