scholarly journals Metabolomics and Biological activities of Chlorella vulgaris grown under modified growth medium (BG11) composition

2020 ◽  
Vol 19 (1) ◽  
pp. 91-123
Author(s):  
Eman A. El-fayoumy ◽  
◽  
Sanaa M. M. Shanab ◽  
Emad A. Shalaby ◽  
◽  
...  
2018 ◽  
Vol 154 ◽  
pp. 01009
Author(s):  
Dianursanti ◽  
Zenitha Lintang Agustin ◽  
Dwini Normayulisa Putri

Chlorella vulgaris and Nannochloropsis oculata are a highly potential microalgae to be used in pilot-scale of biodiesel synthesis. The essential content from these microalgae is the fatty acid of lipid which is the main target for the feed and biodiesel industries. One of the key factor in improving lipid microalgae are the arrangemment of nutrients in the growth medium. Research on the regulation of nutrients using bicarbonate (HCO3-) as an additional inorganic carbon source has been done by many studies, but the yield of lipids obtained has not been much. The aim of the study was to improve the lipid yield of Chlorella vulgaris and Nannochloropsis oculata. Variation of [HCO3-] which added to Walne medium were 25 ppm and 75 ppm, while the Walne medium without the addition of bicarbonate acts as control. The results showed that [HCO3-] 75 ppm could increase Chlorella vulgaris biomass by 0.9162 g/l with 17.0% wt, while Nannochloropsis oculata produced the greatest lipid content in [HCO3-] 25 ppm of 20.3% wt and the largest biomass on [HCO3-] 75 ppm of 1.7233 g/l.


2013 ◽  
Vol 68 (5-6) ◽  
pp. 223-230 ◽  
Author(s):  
Jian-ping Wang ◽  
Abdessamad Debbab ◽  
Catalina Francis Pérez Hemphill ◽  
Peter Proksch

Enniatins are cyclic depsipeptides produced by fungi of the genus Fusarium that are of interest due to their pronounced biological activities; especially enniatins A, A1, B, and B1 possess anticarcinogenic and anti-HIV properties. In the present study, F. tricinctum was grown on seven solid media and in one liquid growth medium with or without addition of peptone or of amino acid precursors in order to identify favourable media with simple cultivation conditions for maximum enniatin production. Additionally, the optimal duration of growth was investigated for the highest yields of enniatins. From the different media analysed, white beans (Phaseolus vulgaris, solid medium) induced the highest accumulation of enniatins A, A1, B, and B1, that reached a maximum of 1,365 mg total enniatins in 1 L growth medium after 18 days of fermentation. Fermentation of F. tricinctum on white beans gave the highest yield of enniatins compared to all other media analysed in this study.


2012 ◽  
Vol 123 ◽  
pp. 366-374 ◽  
Author(s):  
F. Hadj-Romdhane ◽  
P. Jaouen ◽  
J. Pruvost ◽  
D. Grizeau ◽  
G. Van Vooren ◽  
...  

2016 ◽  
Vol 11 (1) ◽  
pp. 53
Author(s):  
Sumarnie Hasto Priyono

Ninety percent from approximately 1200 plant species belonged to Piper genus(Piperaceae) have not been explorated their phytochemical potency and biological activities. Piper miniatum has beeing used traditionally in Papua as spices and tonic. However, the propagation effort has never been done yet so its population in nature is decresed gradually. Therefore, simple propagation is conducted that can be adopted by the local people in order to cultivate as well as to explore its potency as antibacterial agent. The propagation experiment was carried out using air layering method with variable treatment of internode maturity ( R1, R2, R3 ), growth medium (Selaginela sp.,Asplenium nidus root, compost) with completely randomized design with 10 replications.Air layering media using Asplenium nidus root of 18 weeks gave the best respon.Key words: Piper miniatum, Air layering.


2014 ◽  
Vol 2 (1) ◽  
pp. 665-674 ◽  
Author(s):  
Matthew Forrest Blair ◽  
Bahareh Kokabian ◽  
Veera Gnaneswar Gude

Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.


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