Photoacoustic signal recorded by photoacoustic imaging system can be modeled as convolution of initial photoacoustic response by the photoacoustic absorber with the system impulse response. Our goal was to compute the size of photoacoustic absorber using the initial photoacoustic response, deconvolved from the recorded photoacoustic data. For deconvolution, we proposed to use the impulse response of the photoacoustic system, estimated using discrete wavelet transform based homomorphic filtering. The proposed method was implemented on experimentally acquired photoacoustic data generated by different phantoms and also verified by a simulation study involving photoacoustic targets, identical to the phantoms in experimental study. The photoacoustic system impulse response, which was estimated using the acquired photoacoustic signal corresponding to a lead pencil, was used to extract initial photoacoustic response corresponding to a mustard seed of 0.65 mm radius. The recovered radius values of the mustard seed, corresponding to the experimental and simulation studies were 0.6 mm and 0.7 mm.
