The purpose of this study was to evaluate the effects of various root canal treatment methods on bonding strength of a glass-fıber post to root canal dentin. 60 human maxillary anterior teeth were prepared with ParaPost (Coltène) up to diameter of 1.75 mm with drills of 1.14, 1.25, 1.40, 1.50 and 1.75 mm from 1 mm coronal level of apices. The upper surfaces of polished areas were ground to acquire correct angle with the long axis of drills and the specimens were divided into 6 groups (n=10). Glass-fiber posts (1.5mm) (everStick POST, StickTech) were cemented with a dual-cure composite resin cement (ParaCem, Coltène) after following treatment methods. (Group 1) 0.9% isotonic NaCl solution (control), (Group 2) 2.5% NaOCl + Er:YAG Laser (Kavo K-E-Y), (Group 3) 2% Chlorhexidine + Er:YAG Laser , (Group 4) Slurry Bioactive Glass Granules (Vivoxid), (Group 5) Silane-based Primer (3M, ESPE, RelyX) + Er:YAG Laser, (Group 6) Slurry Bioactive Glass Granules + Silane-based Primer + Er:YAG Laser. From each root, two disks of 2 mm thickness was obtained. The push-out test was carried out by using a universal testing device (Lloyd LRX) with a loading tip (diameter 1.7 mm, crosshead speed of 1.0 mm/min) until loosening of the post. Statistical analysis was performed using ANOVA followed by post-hoc comparisons (Tukey B, p<0.05). Root canals were evaluated by SEM. Bond strength of fiber posts were significantly affected by the type of treatment (p<0.05). The highest bond strength was observed in Group 5, whereas the lowest bond strength values were recorded with the Group 3. Bond strength of glass-fiber post to root canal dentin was affected by treatment method. Treatment with laser and Bioactive Glass Granules in combination of using silane yielded the highest bond strength.