scholarly journals Optimization of a-Amylase Production from Bacillus Amyloliquefaciens using Taguchi method

2021 ◽  
Vol 18 (2) ◽  
pp. 337-345
Author(s):  
Pankhuri Sharma ◽  
Shilpa Chapadgaonkar
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Major Effect ◽  
Culture Collection ◽  
Maximum Effect ◽  
Optimization Strategy ◽  
Media Composition ◽  
Amylase Production ◽  
Effect On Yield ◽  
Media Concentration ◽  
Microbial Sources

α-amylase, an enzyme of industrial importance is used extensively in food, pharmaceutical, textile and detergent industries. Since, a substantial quantity of α-amylase isderived from microbial sources, manipulation of bacterial strain, fermentation conditions and media composition has a major effect on yield of enzyme. Bacillus amyloliqifaciens, obtained from MTCC culture collection was used to study the enhancement of α-amylase production using media concentration manipulation. Taguchi’s orthogonal array was designed for maximization of α-amylase output. The different media components selected as parameters to be optimized were calcium chloride, starch, tryptone, ammonium sulphate and glucose. The concentration of starch and tryptone demonstrated to have maximum effect on amylase production. The optimization strategy was successful in obtaining substantial increase in amylase production of about 2 folds as compared to the unoptimized medium.

scholarly journals Assessment of the optimum fertilizer rates and planting density for soybean production in China

2019 ◽  
Author(s):  
Jilong Lv ◽  
Ping He ◽  
Dan Wei ◽  
Xinpeng Xu ◽  
Shaojun Qiu ◽  
...  
Keyword(s):  
Fertilization Rate ◽  
Major Effect ◽  
Optimal Rate ◽  
Planting Density ◽  
High Yield ◽  
Soybean Yield ◽  
Factors Affecting ◽  
K Fertilizer ◽  
P Fertilizer ◽  
Effect On Yield

AbstractFertilization rate and planting density are important factors affecting crop yield. A large number of soybean [Glycine max (L.) Merr] field experimental data (1998-2017) were collected through different database sources to evaluate the optimum fertilizer rate and planting density for high yield of spring and summer soybean in China. The yield of spring and summer soybean gradually increased over year, with their average yields were 2610 and 2724 kg ha−1, respectively. Based on the fitted quadratic curve, the optimal rate of nitrogen (N), phosphorus (P), and potassium (K) fertilizers for high yield of summer soybean was 96 kg N ha−1, 80 kg P2O5 ha−1, and 126 kg K2O ha−1, and the corresponding yields were 3038, 2801 and 2305 kg ha−1, respectively. The optimal rate of N, P and K fertilizers for spring soybean was 71 kg N ha-1, 108 kg P2O5 ha-1 and 74 kg K2O ha−1, and the corresponding yields were 2932, 2834 and 2678 kg ha−1, respectively. The optimum density was 27×104 and 34×104 plants ha−1 under high yield for summer and spring soybean, respectively. Stepwise regression analysis showed that the P fertilizer had the greatest influence on the spring soybean yield followed by K fertilizer and planting density. For summer soybean, population density had the major effect on yield followed by P fertilizer. Overall, the P fertilization and planting density should be payed attention to increase soybean yield in different regions of China.

Cell recycling studies for ?-amylase production by Bacillus amyloliquefaciens

1995 ◽  
Vol 14 (1) ◽  
pp. 23-31 ◽  
Author(s):  
S. Gr�n ◽  
C. Morcel ◽  
C. Emborg ◽  
K. Biedermann
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Amylase Production ◽  
Cell Recycling

Kitchen waste for economical amylase production using Bacillus amyloliquefaciens KCP2

2020 ◽  
Vol 26 ◽  
pp. 101654 ◽  
Author(s):  
Bhumi Bhatt ◽  
Vimal Prajapati ◽  
Kamlesh Patel ◽  
Ujjval Trivedi
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Kitchen Waste ◽  
Amylase Production

scholarly journals Cyclic AMP-dependent protein phosphorylation and insulin secretion in intact islets of Langerhans

1984 ◽  
Vol 218 (1) ◽  
pp. 87-99 ◽  
Author(s):  
M R Christie ◽  
S J H Ashcroft
Keyword(s):  
Insulin Secretion ◽  
Cyclic Amp ◽  
Protein Phosphorylation ◽  
Cholera Toxin ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Sodium Dodecyl ◽  
Major Effect ◽  
Maximum Effect ◽  
Calmodulin Antagonist

Effects on insulin release, cyclic AMP content and protein phosphorylation of agents modifying cyclic AMP levels have been tested in intact rat islets of Langerhans. Insulin release induced by glucose was potentiated by dibutyryl cyclic AMP, glucagon, cholera toxin and 3-isobutyl-1-methylxanthine (IBMX); the calmodulin antagonist trifluoperazine reversed these potentiatory effects. Inhibition by trifluoperazine of IBMX-potentiated release was, however, confined to concentrations of IBMX below 50 microM; higher concentrations, up to 1 mM, were resistant to inhibition by trifluoperazine. IBMX-potentiated insulin release was also inhibited by 2-deoxyadenosine, an inhibitor of adenylate cyclase. In the absence of glucose, IBMX at concentrations up to 1 mM did not stimulate insulin release and in the presence of 3.3 mM-glucose IBMX was effective only at a concentration of 1 mM; under the latter conditions trifluoperazine again did not inhibit insulin secretion. The maximum effect on insulin release was achieved with 25 microM-IBMX. Islet [cyclic AMP] was increased by IBMX, with the maximum rise occurring with 100 microM-IBMX. The increase in [cyclic AMP] elicited by IBMX was more rapid than that induced by cholera toxin. Trifluoperazine did not significantly affect islet cyclic AMP levels under any of the conditions tested. When islets were incubated with [32P]Pi, radioactivity was incorporated into islet ATP predominantly in the gamma-position. The rate of equilibration of label was dependent on medium Pi and glucose concentration and at optimal concentrations of these 100% equilibration of internal [32P]ATP with external [32P]Pi required a period of 3h. Radioactivity was incorporated into islet protein and, in response to an increase in islet [cyclic AMP], the major effect was on a protein of Mr 15 000 on sodium dodecyl sulphate/polyacrylamide gels. The extent of phosphorylation of the Mr-15 000 protein was correlated with the level of cyclic AMP: phosphorylation in response to IBMX was inhibited by 2-deoxyadenosine but not by trifluoperazine. Fractionation of islets suggested that the Mr-15 000 protein was of nuclear origin: the protein co-migrated with histone H3 on acetic acid/urea/Triton gels. In the islet cytosol a number of proteins were phosphorylated in response to elevation of islet [cyclic AMP]: the major species had Mr values of 18 000, 25 000, 34 000, 38 000 and 48 000. Culture of islets with IBMX increased the rate of [3H]-thymidine incorporation.(ABSTRACT TRUNCATED AT 400 WORDS)

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