scholarly journals Cannabis sativa Increases Seizure Severity and Brain Lipid Peroxidation in Pentylenetetrazole-Induced Kindling in Rats

2018 ◽  
Vol 11 (3) ◽  
pp. 1187-1197 ◽  
Author(s):  
Omar M.E. Abdel-Salam ◽  
Amany A. Sleem ◽  
Marawan Abd El Baset Mohamed Sayed ◽  
Yasser A. Khadrawy ◽  
Fatma A. Morsy
Keyword(s):  
Lipid Peroxidation ◽  
Ache Activity ◽  
Cannabis Sativa ◽  
Reduced Glutathione ◽  
Inflammatory Cells ◽  
Brain Lipid ◽  
Glutathione Level ◽  
Significant Elevation ◽  
Control Value ◽  
Brain Lipid Peroxidation

The effect of Cannabis sativa extract on chemical kindling induced in rats by the repeated intraperitoneal (ip) injections of pentylenetetrazole (PTZ) was studied. Rats were treated with PTZ (35 mg/kg) once every 48 hours for 12 times alone or with ip Cannabis sativa (20 mg/kg expressed as Δ9-THC content) 30 min prior to PTZ injection. Seizures were recorded for 20 minutes. Control rats received ip saline. Cannabis treatment caused significant elevation of mean seizure score as compared to PTZ only group after the 5th, 6th and 7th PTZ repeated injections during seizure development. In particular, cannabis caused significant elevation in the frequency of myoclonic jerks, rearing (stage 3), turn over onto one side position and back position (stage 4), and generalized tonic-clonic seizures (stage 5) compared with the PTZ only group. PTZ caused significant elevations in brain lipid peroxidation (malondialdehyde), and nitric oxide along with deceased reduced glutathione level. In addition, brain acetylcholinesterase (AChE) activity significantly decreased compared to control value after PTZ treatment. Cannabis given to PTZ treated rats caused significant increase in brain malondialdehyde and AChE activity compared to PTZ only group. Reduced glutathione level was restored by cannabis. Histopathological studies indicated the presence of spongiform changes, degenerated and necrotic neurons, inflammatory cells, and gliosis in cerebral cortex and degeneration of some Purkinje cells in the cerebellum in both PTZ- and cannabis-PTZ-treated groups. It is concluded that in an epilepsy model induced by repeated PTZ administration, cannabis increased lipid peroxidation and mean seizure score.

Non-steroidal anti-inflammatory drug Ibuprofen modulates brain lipid peroxidation and anti-oxidant enzymes in rat

2020 ◽  
Vol 29 (6) ◽  
pp. 1271-1276
Author(s):  
Ezinwa Hope Chinwe ◽  
Raymond C. Okum ◽  
Ifeanyi Christian Ezeoyili ◽  
Ifeanyi O. Aguzie ◽  
Christopher D. Nwani
Keyword(s):  
Lipid Peroxidation ◽  
Brain Lipid ◽  
Inflammatory Drug ◽  
Anti Oxidant ◽  
Brain Lipid Peroxidation ◽  
Anti Inflammatory

Prenatal malnutrition and lead intake produce increased brain lipid peroxidation levels in newborn rats

2015 ◽  
Vol 19 (7) ◽  
pp. 301-309
Author(s):  
Brenda Gabriela Maldonado-Cedillo ◽  
Araceli Díaz-Ruiz ◽  
Sergio Montes ◽  
Sonia Galván-Arzate ◽  
Camilo Ríos ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Brain Lipid ◽  
Newborn Rats ◽  
Brain Lipid Peroxidation ◽  
Lead Intake ◽  
Prenatal Malnutrition

The effect of quinolinate on rat brain lipid peroxidation is dependent on iron

1997 ◽  
Vol 30 (2) ◽  
pp. 233-237 ◽  
Author(s):  
S STIPEK ◽  
F STASTNY ◽  
J PLATENIK ◽  
J CRKOVSKA ◽  
T ZIMA
Keyword(s):  
Lipid Peroxidation ◽  
Rat Brain ◽  
Brain Lipid ◽  
Brain Lipid Peroxidation

scholarly journals Features of disorders of the system of cytokines and lipid peroxidation in acute ulcerative gastroduodenal hemorrhages

2017 ◽  
Vol 98 (5) ◽  
pp. 686-691 ◽  
Author(s):  
D N Gadzhiev ◽  
V A Allakhverdiev ◽  
N D Gadzhiev
Keyword(s):  
Lipid Peroxidation ◽  
Interleukin 6 ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Malonic Dialdehyde ◽  
Interferon Γ ◽  
Diene Conjugates ◽  
Control Value ◽  
Factor Α ◽  
Necrosis Factor

Aim. Investigation of the local and systemic levels of some pro- and anti-inflammatory cytokines as well as the system of lipid peroxidation - antioxidant defense of the organism in acute ulcerative gastroduodenal hemorrhages, taking into account the severity of the blood loss. Methods. We performed the investigation of cytokines in the blood (tumor necrosis factor α, interferon γ, interleukin-1, -2, -4, -6, -8, -10) and parameters of lipoperoxidation (in plasma - diene conjugates, malonic dialdehyde, catalase; in erythrocytes - reduced glutathione, glutathione peroxidase, total antioxidant activity), interleukin-6 in gastric content and urine, diene conjugates and malonic dialdehyde in biopsy samples from the ulcer edges from patients with acute ulcerative gastroduodenal bleeding. The blood serum of 156 patients with mild (54), moderate (51) and severe (51) bleeding was studied. Interleukin-6 in gastric contents was determined in 37 patients, diene conjugates and malonic dialdehyde in biopsy samples taken from periulcerous zone - in 20 patients. Results. Patients had a statistically significant increase of serum levels of tumor necrosis factor α, interferon γ, interleukins-1, -2, -4, -6, -8, and decrease of interleukin-10 concentration in comparison with the control group. The level of interleukin-6 in gastric juice was 37.5% lower, and in urine - 8.2 times higher than the control value. In plasma and biopsy samples, the content of diene conjugates and malonic dialdehyde was statistically significantly higher, in erythrocytes the level of reduced glutathione was lower than the control value. The depth of imbalance in the system of cytokines and lipid peroxidation - antioxidant defense increased with increasing of blood loss severity. Conclusion. The relationship between cytokine production and lipid peroxidation indices is established, which confirms the role of local and systemic disorders of cytokine and antioxidant status in the occurrence of ulcer bleeding.

scholarly journals Brain Levels of Reduced Glutathione and Malondialdehyde in Honey-Fed Wistar Rats

2021 ◽  
Vol 18 (3) ◽  
pp. 517-522
Author(s):  
Prosper Ejiro Awhin ◽  
Ugochukwu Enyinnaya Uzuegbu ◽  
Evelyn Ojugbeli ◽  
Lily Oghenevovwero Otomewo
Keyword(s):  
Wistar Rats ◽  
Biochemical Analysis ◽  
Reduced Glutathione ◽  
Brain Lipid ◽  
Brain Levels ◽  
Female Wistar Rats ◽  
Mda Content ◽  
Simultaneous Decrease ◽  
Brain Lipid Peroxidation ◽  
Natural Honey

This research sought to verify the effect of natural honey on brain levels of malondialdehyde (MDA) and reduced glutathione (GSH) in rats. Forty nine male and female Wistar rats were used for the experiment. The rats were allotted into seven groups of seven rats in each group. For one month, rats in groups 1-4 were fed with 100% feed, 20%, 30% and 40% honey respectively. The remaining 3 groups were fed with amounts of refined fructose and glucose equivalent to those in 20%, 30% and 40% honey. The brains were then excised, homogenized and used for biochemical analysis. Results showed that honey in all concentrations caused a significant increase in GSH levels but only 20% honey caused a significant decrease in MDA level when compared with control. Also, fructose feeding at 20%, 30% and 40% increased both brain GSH and MDA levels. Consequently, the influence of GSH as an antioxidant against brain lipid peroxidation needs further studies for better understanding since an increase in GSH for fructose- and honey-fed rats did not cause a simultaneous decrease in MDA content.

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