scholarly journals Brain Levels of Reduced Glutathione and Malondialdehyde in Honey-Fed Wistar Rats

2021 ◽  
Vol 18 (3) ◽  
pp. 517-522
Author(s):  
Prosper Ejiro Awhin ◽  
Ugochukwu Enyinnaya Uzuegbu ◽  
Evelyn Ojugbeli ◽  
Lily Oghenevovwero Otomewo
Keyword(s):  
Wistar Rats ◽  
Biochemical Analysis ◽  
Reduced Glutathione ◽  
Brain Lipid ◽  
Brain Levels ◽  
Female Wistar Rats ◽  
Mda Content ◽  
Simultaneous Decrease ◽  
Brain Lipid Peroxidation ◽  
Natural Honey

This research sought to verify the effect of natural honey on brain levels of malondialdehyde (MDA) and reduced glutathione (GSH) in rats. Forty nine male and female Wistar rats were used for the experiment. The rats were allotted into seven groups of seven rats in each group. For one month, rats in groups 1-4 were fed with 100% feed, 20%, 30% and 40% honey respectively. The remaining 3 groups were fed with amounts of refined fructose and glucose equivalent to those in 20%, 30% and 40% honey. The brains were then excised, homogenized and used for biochemical analysis. Results showed that honey in all concentrations caused a significant increase in GSH levels but only 20% honey caused a significant decrease in MDA level when compared with control. Also, fructose feeding at 20%, 30% and 40% increased both brain GSH and MDA levels. Consequently, the influence of GSH as an antioxidant against brain lipid peroxidation needs further studies for better understanding since an increase in GSH for fructose- and honey-fed rats did not cause a simultaneous decrease in MDA content.

scholarly journals Changes in Antioxidant Markers and Histopathology of Wistar Rats Fed with Edible Clay (Nzu)

2021 ◽  
pp. 16-20
Author(s):  
A. Dokubo ◽  
A. E. Okwudike ◽  
K. T. Nwauche
Keyword(s):  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Oxygen Species ◽  
Histopathological Changes ◽  
Male And Female ◽  
Female Wistar Rats ◽  
Group A ◽  
Continuous Feeding ◽  
Antioxidant Markers

The changes in the antioxidant markers and histopathology in both adult male and female wistar rats fed with Calabash Chalk (nzu) was investigated. Twenty (20) wistar rats weighing between (120-150 g) were used for this study. They were randomly divided into four (4) Groups containing five (5) animals each (n=5) A-D. Groups (B-D) were fed with 1.0%, 2.0% and 8.0% of clay. Group A, not fed with clay served as control. After 21 days of continuous feeding, the animals were sacrificed and their liver organs excised for the following antioxidant markers (Catalase CAT, Reduced Glutathione GSH, Superoxide dismutase, SOD) and histopathology. The results showed significant (p< 0.05) increase in catalase activity in rats fed with 1.0% and 8.0% clay compared to control (0 clay) and group fed with 2.0%. However, no significant (p>0.05) difference was obtained for the group fed with 2.0% when compared to control.  The results obtained for GSH and SOD also showed no significant (p>0.05) difference in the fed groups when compared to control. Histopathological changes indicated mild periportal and intraparenchymal inflammation in group fed with 8.0%. From the study it can be deduced that clay consumption has the potential to elicit the activities of antioxidant markers and subsequent depletion providing weak defenses against reactive oxygen species (ROS) and liver damage in the rats.

scholarly journals Cannabis sativa Increases Seizure Severity and Brain Lipid Peroxidation in Pentylenetetrazole-Induced Kindling in Rats

2018 ◽  
Vol 11 (3) ◽  
pp. 1187-1197 ◽  
Author(s):  
Omar M.E. Abdel-Salam ◽  
Amany A. Sleem ◽  
Marawan Abd El Baset Mohamed Sayed ◽  
Yasser A. Khadrawy ◽  
Fatma A. Morsy
Keyword(s):  
Lipid Peroxidation ◽  
Ache Activity ◽  
Cannabis Sativa ◽  
Reduced Glutathione ◽  
Inflammatory Cells ◽  
Brain Lipid ◽  
Glutathione Level ◽  
Significant Elevation ◽  
Control Value ◽  
Brain Lipid Peroxidation

The effect of Cannabis sativa extract on chemical kindling induced in rats by the repeated intraperitoneal (ip) injections of pentylenetetrazole (PTZ) was studied. Rats were treated with PTZ (35 mg/kg) once every 48 hours for 12 times alone or with ip Cannabis sativa (20 mg/kg expressed as Δ9-THC content) 30 min prior to PTZ injection. Seizures were recorded for 20 minutes. Control rats received ip saline. Cannabis treatment caused significant elevation of mean seizure score as compared to PTZ only group after the 5th, 6th and 7th PTZ repeated injections during seizure development. In particular, cannabis caused significant elevation in the frequency of myoclonic jerks, rearing (stage 3), turn over onto one side position and back position (stage 4), and generalized tonic-clonic seizures (stage 5) compared with the PTZ only group. PTZ caused significant elevations in brain lipid peroxidation (malondialdehyde), and nitric oxide along with deceased reduced glutathione level. In addition, brain acetylcholinesterase (AChE) activity significantly decreased compared to control value after PTZ treatment. Cannabis given to PTZ treated rats caused significant increase in brain malondialdehyde and AChE activity compared to PTZ only group. Reduced glutathione level was restored by cannabis. Histopathological studies indicated the presence of spongiform changes, degenerated and necrotic neurons, inflammatory cells, and gliosis in cerebral cortex and degeneration of some Purkinje cells in the cerebellum in both PTZ- and cannabis-PTZ-treated groups. It is concluded that in an epilepsy model induced by repeated PTZ administration, cannabis increased lipid peroxidation and mean seizure score.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

Oral in vivo Bactofection in Dextran Sulfate Sodium Treated Female Wistar Rats

2010 ◽  
Vol 58 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Roland Pálffy ◽  
Michal Behuliak ◽  
Roman Gardlík ◽  
Peter Jáni ◽  
L'udevít Kádaši ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Female Wistar Rats

Renal Cortical Slices: An In Vitro Model for Kidney Metabolism and Toxicity

1992 ◽  
Vol 20 (1) ◽  
pp. 71-76
Author(s):  
Andrea Trevisan ◽  
Stefano Maso ◽  
Paola Meneghetti
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
Organic Anion ◽  
Cortical Slice ◽  
Lactate Dehydrogenase Release ◽  
Age Related ◽  
Renal Cortical Slice ◽  
Male Wistar Rats ◽  
Vitro Model

The in vitro renal cortical slice model was used to study: 1) the effects on the kidney of some haloalkanes and haloalkenes using 3-month-old male Wistar rats; 2) influence of age and sex on renal cortical slice indices in non-treated rats; and 3) effects of 1,2-dichloropropane on the slices after pretreatment of 3-month-old male Wistar rats with DL-butathionine-[S,R]-sulphoximine. The most nephrotoxic chemical used was 1,3-dichloropropene, which caused a total depletion in the levels of reduced glutathione, a high peroxidation of lipid (about three thousand-fold with respect to control), a significant release of tubular enzymes into the medium, and loss of organic anion ( p-aminohippurate) accumulation. All the chemicals affected the cytosol more than the brush border. The most remarkable age-related differences in the untreated slices were the progressive decrease of reduced glutathione (p<0.05 from three months of age), and an increase in lactate dehydrogenase release into the medium (p<0.05 from six months of age). By contrast, sex differences were slight. The ‘treatment with 1,2-dichloropropane of slices prepared from rats pretreated with DL-butathionine-[S,R]-sulphoximine significantly increased the depletion of glutathione content (p<0.05) and malondialdehyde release in the medium (p<0.001) caused by the solvent alone.

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