scholarly journals High Performance Thin Layer Chromatographic Quantification Domperidone and Paracetamol in Tablets Forms Domperidone, Paractamol, Silica gel HPTLC Plate, HPTL

2014 ◽  
Vol 30 (1) ◽  
pp. 395-399 ◽  
Author(s):  
Amar Singh ◽  
M Khan
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
High Performance ◽  
Hptlc Plate

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

A HPTLC Densitometer Determination of Sinapic acid in Chandrasur (Lepidium sativum)

2008 ◽  
Vol 1 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Preeti Sagar Nayak ◽  
Sunil D Upadhyaya ◽  
Anubha Upadhyaya
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
High Performance ◽  
Sinapic Acid ◽  
Lepidium Sativum ◽  
Analysis Method ◽  
Qualitative And Quantitative Analysis ◽  
Quantitative Analysis Method ◽  
Acetic Acid Water

A qualitative and quantitative analysis method was established to improve quality assessment standards for Lepidium sativum seeds. The sinapic acid was identified by comparing with standards and quantified simultaneously by High performance thin layer chromatographic (HPTLC). HPTLC of Lepidium sativum methanolic extract was performed on Silica gel 60F254 [20 cm × 10 cm] plates with butanol: acetic acid: water (4:1:5), as mobile phase. Quantitative evaluation of the plate was performed in the absorbance-reflectance mode at 326 nm. The sinapic acid was separated on a thin layer of silica gel and determined by HPTLC-photo densitometry. The proposed method is simple, sensitive and can be used for the routine assay. Keywords: Lepidium sativum; Sinapic acid; HPTLC; Photo densitometry. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1196  

Simple, Rapid Cleanup Method for Analysis of Aflatoxins and Comparison with Various Methods

1985 ◽  
Vol 68 (3) ◽  
pp. 458-461 ◽  
Author(s):  
Hisashi Kamimura ◽  
Motohiro Nishijima ◽  
Kazuo Yasuda ◽  
Hirofumi Ushiyama ◽  
Setsuko Tabata ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Rapid Determination ◽  
Hptlc Plate ◽  
Simple Method ◽  
Florisil Column ◽  
Chromatographic Procedure ◽  
Test Materials ◽  
Column Chromatographic

Abstract A method is described for simple and rapid determination of aflatoxins in corn, buckwheat, peanuts, and cheese. Aflatoxins were extracted with chloroform-water and were purified by a Florisil column chromatographic procedure. Column eluates were concentrated and spotted on a high performance thin layer chromatographic (HPTLC) plate, which was then developed in chloroform-acetone (9 + 1) and/or ethermethanol- water (94 + 4.5 + 1.5)orchloroform-isopropanol-acetone (85 + 5 + 10). Each aflatoxin was quantitated by densitometry. The minimum detectable aflatoxin concentrations (μg/kg) in various test materials were 0.2, B1; 0.1, B2; 0.2, G1; 0.1, G2; and 0.1,M1.Recoveries of the aflatoxins added to corn, peanut, and cheese samples at 10-30 μg/kg were > 69% (aflatoxin G2) and averaged 91%, B1; 89%, B2; 91%, G1; 78%, G2; and 92%, M1. The simple method described was compared with the AOAC CB method, AOAC BF method, and AOAC milk and cheese method. These methods were applied to corn, peanut, and cheese composites spiked with known amounts of aflatoxins, and to naturally contaminated buckwheat and cheese. Recoveries were much lower for the BF method compared with our simple method and the CB method.

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