Utilization of germinated broad bean seeds as an oviposition substrate in mass rearing of the predatory bug, Orius sauteri (Poppius) (Heteroptera: Anthocoridae).

Some details on the biology, behavior and laboratory mass rearing of Anastrepha obliqua are offered. Information on larvaI diets and oviposition substrates are discussed. Eggs of A. obliqua are, very succeptible to dehydratation and they collapse just few minutes after oviposition, if substrate for oviposition is not near 100% R.H. When using fruits as oviposition substrate, smallerfruit species, Spanish plums (Spondias spp.) offer higher yields. Bigger fruits (mango) loose large amounts of water and it accumulates in the sand substrate drowning mature larvae. After analyzing the biological cycle, the weaker part seems to be the 20 minute period in which mature larvae abandon the fallen fruit substrate and crawl few a centimeters on the floor seeking for an appropiate place for ovipositing. In this period large amounts of predators easily diminish larval population. Once pupation takes place, adult forms hatch in small groups, after eleven days, when atmosferic relativehumidity reaches 70%. Another observations are also included.

Download Full-text

Facilitated harvesting of eggs from laboratory-reared Chrysoperla externa (Neuroptera: Chrysopidae)

Revista Colombiana de Entomología ◽

10.25100/socolen.v42i2.6683 ◽

2016 ◽

Vol 42 (2) ◽

pp. 133 ◽

Cited By ~ 3

Author(s):

Ana Luiza Viana Sousa ◽

Brígida Souza ◽

Carlos Eduardo Souza Bezerra ◽

Bruno Barbosa Amaral

Keyword(s):

Complete Removal ◽

Mass Rearing ◽

Growth Chamber ◽

Embryonic Stage ◽

Control Programs ◽

Microtiter Plates ◽

Oviposition Substrate ◽

Stage Development ◽

Chemical Techniques ◽

Egg Destruction

Chrysoperla externa (Neuroptera: Chrysopidae) eggs are attached to the oviposition substrate by long silk stalks. The complete removal of these stalks is crucial for efficient egg release in biological control programs. The present study aimed at establishing an appropriate oviposition substrate and determining the best embryonic stage for submission of C. externa eggs to manual destalking and harvesting. Eggs oviposited on bond or chamois paper substrates were transferred from rearing cages and incubated in a growth chamber under controlled conditions for 24, 48, 72 or 96 hours according to the embryonic stage development required. Substrates were positioned in an inclined tray and softly brushed with a folded rectangle of soft muslin cloth. Destalked eggs were placed individually in microtiter plates and incubated in a growth chamber until hatching. Egg destruction at all embryonic stages and oviposition on the chamois substrate were considerably higher as compared to those from bond paper. Young eggs harvested from chamois paper were particularly susceptible and exhibited 88 % destruction, whereas eggs aged 48, 72 or 96 hours showed < 10 % destruction on both substrates. Viability of eggs collected at 24 hours for both substrates was significantly different from the observed for the other embryonic stages. The method described will contribute to improve the efficiency of manual harvesting of C. externa eggs and can be employed as an alternative to chemical techniques of destalking in mass rearing.

Download Full-text

Basic Studies Aiming at Orius minutus (Hemiptera: Anthocoridae) Mass-Rearing

Insects ◽

10.3390/insects13010077 ◽

2022 ◽

Vol 13 (1) ◽

pp. 77

Author(s):

Hye-Jeong Jun ◽

Kyoung-Su Kim ◽

Eun-Hye Ham

Keyword(s):

Brine Shrimp ◽

Biological Control Agent ◽

Mass Rearing ◽

Control Agent ◽

Artificial Substrates ◽

Ephestia Cautella ◽

Control Group ◽

Significant Difference ◽

Oviposition Substrate ◽

Artificial Oviposition Substrate

This study presented biological and economic data for the mass-rearing of Orius minutus in Korea. Simplifying the mass-rearing process through an alternative diet and an artificial oviposition substrate is a prerequisite for enhancing the usability of this insect as a biological control agent. We compare the hatch rate of O. minutus eggs deposited on a plant substrate with that of eggs deposited on two artificial substrates, cork sheets and rubber. The results indicate that cork sheet is the most cost-effective artificial oviposition substrate for the mass-rearing of O. minutus. We also examine five feeding treatments that included two types of brine shrimp eggs and eggs of Ephestia cautella to compare the number of eggs laid in the fifth generation. We found no significant difference between the two treatment groups; 61.3 eggs were laid in the treatment group fed iron-coated brine shrimp and moth eggs, and 67.4 eggs were laid in the control group. The plant-free model developed in our study can reduce rearing costs by 70.5% compared to the conventional mass-rearing model.

Download Full-text

Developing apple leafcurling midge rearing techniques

New Zealand Plant Protection ◽

10.30843/nzpp.2018.71.201 ◽

2018 ◽

Vol 71 ◽

pp. 353

Author(s):

Amanda J. Hawthorne ◽

Dominic E. Hartnett

Keyword(s):

Mass Rearing ◽

Day Length ◽

Controlled Environment ◽

Ambient Conditions ◽

Export Markets ◽

Long Day ◽

Large Numbers ◽

Oviposition Substrate ◽

Rearing Techniques ◽

Dasineura Mali

Apple leafcurling midge (Dasineura mali; ALCM) is considered a quarantine pest in some of New Zealand’s valuable export markets. Research into alternatives to methyl bromide as a fumigant requires a mass rearing programme to provide large numbers of all life stages. Our aim was to focus on developing an understanding of the environmental conditions that break ALCM diapause and facilitate ALCM oviposition. A trial was conducted comparing ALCM oviposition rates on apple seedlings in a controlled temperature room versus a shade house in ambient conditions over the summer months. Additionally, long day length and high humidity conditions were tested to break the diapause of ALCM cocooning larvae. Oviposition rates on young apple seedlings were similar in the controlled environment room and the shade house; however, apple seedlings grew better in the shade house due to lower incidence of powdery mildew. Adults emerged from ALCM cocoons that had been in diapause for five months and then held at 20.9Â°C for seven days. Conditions to break ALCM diapause have been determined. Seedlings at the six- to eight-leaf stage have the potential to provide an oviposition substrate. Further investigations are required into artificial oviposition substrates and the development of a larval diet to mass rear ALCM larvae.

Download Full-text