Real-Time Detection of Escherichia coli in Water Pipe Using a Microfluidic Device with One-Step Latex Immunoagglutination Assay

2009 ◽  
Vol 52 (3) ◽  
pp. 1031-1039
Author(s):  
J.-Y. Yoon ◽  
J.-H. Han ◽  
C. Y. Choi ◽  
M. Bui ◽  
R. G. Sinclair
2021 ◽  
Vol MA2021-01 (55) ◽  
pp. 1415-1415
Author(s):  
Rocio Arreguin Arreguin Campos ◽  
Kasper Eersels ◽  
Hanne Diliën ◽  
Bart van Grinsven ◽  
Thomas J. Cleij

2013 ◽  
Author(s):  
Samhwan Kim ◽  
Jongmoon Jang ◽  
Hongsoo Choi ◽  
Cheil Moon

2008 ◽  
Vol 71 (7) ◽  
pp. 1469-1474 ◽  
Author(s):  
FAITH J. CRITZER ◽  
DORIS H. D'SOUZA ◽  
DAVID A. GOLDEN

Expression of the multiple antibiotic resistance (mar) operon causes increased antimicrobial resistance in bacterial pathogens. The activator of this operon, MarA, can alter expression of >60 genes in Escherichia coli K-12. However, data on the expression of virulence and resistance genes when foodborne pathogens are exposed to antimicrobial agents are lacking. This study was conducted to determine transcription of marA (mar activator), stx1 (Shiga toxin 1), and eaeA (intimin) genes of E. coli O157:H7 EDL933 as affected by sodium benzoate. E. coli O157:H7 was grown in Luria-Bertani broth containing 0 (control) and 1% sodium benzoate at 37°C for 24 h, and total RNA was extracted. Primers were designed for hemX (209 bp; housekeeping gene), marA (261 bp), and eaeA (223 bp) genes; previously reported primers were used for stx1. Tenfold dilutions of RNA were used in a real-time one-step reverse transcriptase PCR to determine transcription levels. All experiments were conducted in triplicate, and product detection was validated by gel electrophoresis. For marA and stx1, real-time one-step reverse transcriptase PCR products were detected at a 1-log-greater dilution in sodium benzoate–treated cells than in control cells, although cell numbers for each were similar (7.28 and 7.57 log CFU/ml, respectively). This indicates a greater (albeit slight) level of their transcription in treated cells than in control cells. No difference in expression of eaeA was observed. HemX is a putative uroporphyrinogen III methylase. The hemX gene was expressed at the same level in control and treated cells, validating hemX as an appropriate housekeeping marker. These data indicate that stx1 and marA genes could play a role in pathogen virulence and survival when treated with sodium benzoate, whereas eaeA expression is not altered. Understanding adaptations of E. coli O157:H7 during antimicrobial exposure is essential to better understand and implement methods to inhibit or control survival of this pathogen in foods.


2014 ◽  
Vol 41 (12) ◽  
pp. 1215003
Author(s):  
黄庶识 Huang Shushi ◽  
卢明倩 Lu Mingqian ◽  
李冰 Li Bing ◽  
周冰 Zhou Bing ◽  
陈丽梅 Chen Limei

2020 ◽  
Vol 12 (32) ◽  
pp. 36458-36467 ◽  
Author(s):  
Marta Lafuente ◽  
Ismael Pellejero ◽  
Alberto Clemente ◽  
Miguel A. Urbiztondo ◽  
Reyes Mallada ◽  
...  

Nanoscale ◽  
2019 ◽  
Vol 11 (38) ◽  
pp. 17689-17698 ◽  
Author(s):  
Johann Mertens ◽  
Ana Cuervo ◽  
José L. Carrascosa

Variation in fluctuation of the bacteria membrane is measured by the coupling to an elastic microcantilever plate. We report the real time detection of E.coli infection by bacteriophage T7 using this method.


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