scholarly journals Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

2018 ◽  
Vol 19 (2) ◽  
pp. 626-631
Author(s):  
PASKAH PARTOGI AGUNG ◽  
WIDYA PINTAKA BAYU PUTRA ◽  
SAIFUL ANWAR ◽  
ARI SULISTYO WULANDARI ◽  
SYAHRUDDIN SAID ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Single Nucleotide Polymorphisms ◽  
Bos Indicus ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Association of single nucleotide polymorphisms in the insulin and growth hormone gene with growth traits of Mia Chicken

2019 ◽  
Author(s):  
Nguyen Hoang Thinh ◽  
Hoang Anh Tuan ◽  
Nguyen Thi Vinh ◽  
Bui Huu Doan ◽  
Nguyen Thi Phuong Giang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Marker Gene ◽  
Average Daily Gain ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Chicken Breed ◽  
Gh Gene

This study was conducted in Mia chicken breed to evaluate the association between four single nucleotide polymorphisms (SNPs), in insulin (INS) and growth hormone (GH) genes, with growth traits. Three genotypes for the SNP A3971G of INS gene and the SNP G662A and C423T of GH gene were present in the population while only two genotypes were found in the Mia chicken breed for SNP T3737C of INS gene (TT and TC). The SNP T3737C INS gene and G662A GH gene had significant association with growth traits (P less than 0.05). A significant association of T3737C INS gene with body weight (BW) was observed at 10 to 12 weeks of age and average daily gain (ADG) at 6-8 weeks of age. The SNP G662A of the GH gene was significantly associated (P less than 0.05) with BW of Mia chicken at ages from 7 to 14 weeks and with ADG (4-6; 6-8; 8-10; 10-12 and 2-16 weeks). Chicken with the GG genotype had greater BW and ADG compared to the other genotypes. The results demonstrated that this SNP G662A GH gene may be used as a candidate marker gene for genetic improvement of growth traits in Mia chicken breed.

Single nucleotide polymorphisms in the growth hormone gene ofOreochromis niloticusand their association with growth performance

2017 ◽  
Vol 48 (12) ◽  
pp. 5835-5845 ◽  
Author(s):  
Suhaila Karim Khalil Jaser ◽  
Marco Aurélio Dessimoni Dias ◽  
Aline de Assis Lago ◽  
Rafael Vilhena Reis Neto ◽  
Alexandre Wagner Silva Hilsdorf
Keyword(s):  
Growth Hormone ◽  
Single Nucleotide Polymorphisms ◽  
Growth Performance ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals PSXII-30 SNPs IN THE GROWTH HORMONE GENE OF WILD AND DOMESTIC REINDEER (Rangifer tarandus) IN RUSSIA

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 241-242
Author(s):  
Anna Krutikova ◽  
Natalia Dementeva
Keyword(s):  
Growth Hormone ◽  
Cervus Elaphus ◽  
Rangifer Tarandus ◽  
Genetic Difference ◽  
Live Weight ◽  
Proteinase K ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Climatic Zones

Abstract In the northern part of Russia, there are wild and domestic reindeer. There are four breeds of domestic reindeer in Russia: Nenets, Evenki, Even and Chukchi. The breeds are adapted to various climatic zones of habitat (tundra or taiga). Breeds vary in growth and live weight, as well as in areas of application. The aim was to study the polymorphism of the gene for growth hormone in the reindeer. The DNA was extracted by the phenolic method using the standard method using proteinase K from blood and tissues. Specially designed primers were used to amplify the site of growth hormone gene. As a result of amplification, a 422 bp fragment was obtained covering the region of the somatotropin gene containing 2 and 3 exons. The resulting amplification was sequenced on an Applied Biosystems 3500 Genetic Analyzer using the BigDye® Terminator v3.1 Sequencing Standard Kit according to the protocol. Four single-nucleotide polymorphisms of C12T, C72T, A122G, A235G were detected in the investigated region of the growth hormone gene (somatotropin). The positions of the found SNPs were determined from a similar section of the gene in a red deer (Cervus elaphus), the genome of which was completely sequenced. Two SNPs are in the intron, two are in the exon. The frequencies of genotypes and alleles were calculated in the six studied populations of domestic and wild reindeer. The results obtained in the study make it possible to draw conclusions about the genetic difference between the populations of wild and domestic reindeer of Russia, and between the domestic reindeer of different breeds. Theme АААА-А-18-118021590138-1.

scholarly journals Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

2021 ◽  
Vol 12 ◽  
Author(s):  
Marie-Christine Bartens ◽  
Amanda J. Gibson ◽  
Graham J. Etherington ◽  
Federica Di Palma ◽  
Angela Holder ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Bos Indicus ◽  
Full Length ◽  
Nucleotide Polymorphisms ◽  
Wild Type ◽  
Single Nucleotide ◽  
Brown Swiss ◽  
Cattle Breeds ◽  
Species Specific ◽  
Mycobacterial Antigens

Recent evidence suggests that several cattle breeds may be more resistant to infection with the zoonotic pathogen Mycobacterium bovis. Our data presented here suggests that the response to mycobacterial antigens varies in macrophages generated from Brown Swiss (BS) and Holstein Friesian (HF) cattle, two breeds belonging to the Bos taurus family. Whole genome sequencing of the Brown Swiss genome identified several potential candidate genes, in particular Toll-like Receptor-2 (TLR2), a pattern recognition receptor (PRR) that has previously been described to be involved in mycobacterial recognition. Further investigation revealed single nucleotide polymorphisms (SNP) in TLR2 that were identified between DNA isolated from cells of BS and HF cows. Interestingly, one specific SNP, H326Q, showed a different genotype frequency in two cattle subspecies, Bos (B.) taurus and Bos indicus. Cloning of the TLR2 gene and subsequent gene-reporter and chemokine assays revealed that this SNP, present in BS and Bos indicus breeds, resulted in a significantly higher response to mycobacterial antigens as well as tri-acylated lipopeptide ligands in general. Comparing wild-type and H326Q containing TLR2 responses, wild-type bovine TLR2 response showed clear, diminished mycobacterial antigen responses compared to human TLR2, however bovine TLR2 responses containing H326Q were found to be partially recovered compared to human TLR2. The creation of human:bovine TLR2 chimeras increased the response to mycobacterial antigens compared to the full-length bovine TLR2, but significantly reduced the response compared to the full-length human TLR2. Thus, our data, not only present evidence that TLR2 is a major PRR in the mammalian species-specific response to mycobacterial antigens, but furthermore, that there are clear differences between the response seen in different cattle breeds, which may contribute to their enhanced or reduced susceptibility to mycobacterial infection.

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