Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits

This study was conducted in Mia chicken breed to evaluate the association between four single nucleotide polymorphisms (SNPs), in insulin (INS) and growth hormone (GH) genes, with growth traits. Three genotypes for the SNP A3971G of INS gene and the SNP G662A and C423T of GH gene were present in the population while only two genotypes were found in the Mia chicken breed for SNP T3737C of INS gene (TT and TC). The SNP T3737C INS gene and G662A GH gene had significant association with growth traits (P less than 0.05). A significant association of T3737C INS gene with body weight (BW) was observed at 10 to 12 weeks of age and average daily gain (ADG) at 6-8 weeks of age. The SNP G662A of the GH gene was significantly associated (P less than 0.05) with BW of Mia chicken at ages from 7 to 14 weeks and with ADG (4-6; 6-8; 8-10; 10-12 and 2-16 weeks). Chicken with the GG genotype had greater BW and ADG compared to the other genotypes. The results demonstrated that this SNP G662A GH gene may be used as a candidate marker gene for genetic improvement of growth traits in Mia chicken breed.

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Single Nucleotide Polymorphisms in Growth Hormone Gene and Their Association with Growth Traits in Siniperca chuatsi (Basilewsky)

International Journal of Molecular Sciences ◽

10.3390/ijms15047029 ◽

2014 ◽

Vol 15 (4) ◽

pp. 7029-7036 ◽

Cited By ~ 23

Author(s):

Changxu Tian ◽

Min Yang ◽

Liyuan Lv ◽

Yongchao Yuan ◽

Xufang Liang ◽

...

Keyword(s):

Growth Hormone ◽

Single Nucleotide Polymorphisms ◽

Growth Traits ◽

Growth Hormone Gene ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Siniperca Chuatsi

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Single-nucleotide polymorphisms in FABP4 gene associated with growth traits in Egyptian sheep

Veterinary World ◽

10.14202/vetworld.2020.1126-1132 ◽

2020 ◽

Vol 13 (6) ◽

pp. 1126-1132

Author(s):

Heba Ibrahim Shafey ◽

Karima Fathy Mahrous ◽

Amal Ahmed Mohamed Hassan ◽

Hossam Eldin Rushdi ◽

Mohamed Abd El-Aziz Mohamed Ibrahim

Keyword(s):

Single Nucleotide Polymorphisms ◽

Growth Traits ◽

Average Daily Gain ◽

Correlation Coefficients ◽

Nucleotide Polymorphisms ◽

Negative Effects ◽

Single Nucleotide ◽

Fatty Acid Binding ◽

Sheep Breeds ◽

Intron 1

Aim: The present study was performed to assess the association of single-nucleotide polymorphisms (SNPs) in the fatty acid-binding protein 4 (FABP4) gene with birth weight (BW), final weight (FW), and average daily gain (ADG) in three Egyptian sheep breeds. Materials and Methods: Genomic DNA was extracted from the blood samples of 50 male and female individuals representing Ossimi, Rahmani, and Barki sheep breeds. A 407 bp nucleotide (nt) segment from the first intron of FABP4 was amplified by polymerase chain reaction, sequenced, and analyzed in the different samples. Results: Sequence analysis of the determined segment (407 bp) revealed four SNPs (all transition types) at nt position 372 (CP011894.1:g.57605471) A>G, nt position 211 (CP011894.1:g.57605632) A>G, nt position 143 (CP011894.1:g.57605700) T>C, and nt position 111 (CP011894.1:g.57605732) T>C. The allelic and genotypic frequencies for the identified SNPs in the sheep breeds were calculated. At nt positions 372 and 211, two alleles were identified (A and G). Only two genotypes were present at nt position 372 (AA and AG), while three genotypes were present at nt position 211 (AA, AG, and GG). Two alleles (T and C) and three identified genotypes (TT, TC, and CC) were detected at nt positions 143 and 111. Analysis of the results revealed that AA genotype at nt position 372 is associated with higher estimates for BW, FW, and ADG when compared to all the other genotypes. Very high correlation coefficients were found between the genotypes 143-TT and 111-TT and also between 143-TC and 111-TC. The genotypes 372-AG, 211-GG, 211-AA, 143-TT, 143-CC, 111-TT, 111-TC, and 111-CC were associated with negative effects on BW, FW, and ADG. Conclusion: The detection of four SNPs in a partial sequence of the Egyptian ovine FABP4 gene intron 1 reflected that this gene harbors substantial diversity. In addition, a novel SNP at nt position 372 (CP011894.1:g.57605471) A>G was associated with higher estimates for BW, FW, and ADG.

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Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein-Friesian dairy cattle

Journal of Dairy Science ◽

10.3168/jds.2010-3385 ◽

2010 ◽

Vol 93 (12) ◽

pp. 5959-5969 ◽

Cited By ~ 22

Author(s):

M.P. Mullen ◽

D.P. Berry ◽

D.J. Howard ◽

M.G. Diskin ◽

C.O. Lynch ◽

...

Keyword(s):

Growth Hormone ◽

Single Nucleotide Polymorphisms ◽

Dairy Cattle ◽

Bos Taurus ◽

Growth Hormone Gene ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Holstein Friesian ◽

Performance Traits ◽

And Performance

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Analysis of HorseMyostatinGene and Identification of Single Nucleotide Polymorphisms in Breeds of Different Morphological Types

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/542945 ◽

2010 ◽

Vol 2010 ◽

pp. 1-11 ◽

Cited By ~ 19

Author(s):

Stefania Dall'Olio ◽

Luca Fontanesi ◽

Leonardo Nanni Costa ◽

Marco Tassinari ◽

Laura Minieri ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Tata Box ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Analysis Of Molecular Variance ◽

Molecular Variance ◽

Intron 1 ◽

Significant Difference ◽

Intron 2 ◽

Negative Modulator

Myostatin (MSTN) is a negative modulator of muscle mass. We characterized the horse (Equus caballus)MSTNgene and identified and analysed single nucleotide polymorphisms (SNPs) in breeds of different morphological types. Sequencing of coding, untranslated, intronic, and regulatory regions ofMSTNgene in 12 horses from 10 breeds revealed seven SNPs: two in the promoter, four in intron 1, and one in intron 2. The SNPs of the promoter (GQ183900:g.26TC and GQ183900:g.156TC, the latter located within a conserved TATA-box like motif) were screened in 396 horses from 16 breeds. The g.26C and the g.156C alleles presented higher frequency in heavy (brachymorphic type) than in light breeds (dolichomorphic type such as Italian Trotter breed). The significant difference of allele frequencies for the SNPs at the promoter and analysis of molecular variance (AMOVA) on haplotypes indicates that these polymorphisms could be associated with variability of morphology traits in horse breeds.

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