Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

Abstract. Susari NNW, Suastika P, Agustina KK. 2021. Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia. Biodiversitas 22: 165-172. Cytochrome oxidase subunit I (COI) is one of the molecular markers often used as a differentiator with many advantages in phylogeny analysis, and it thus rarely undergoes substitution. This research was conducted to characterize the genetics of Taro and Bali (Bos javanicus) cattle. Blood from the animals was collected from the jugularis vein and amplified by PCR. The target area was COI with a primer that was successfully amplified, namely the forward BICOIF (5'-TTC-TCAACCAACCATAAAGATATTGG-3') and the reverse BICOIR fragment (5'-TAG-ACTTCGGGGTGTCCAAAGAATCA-3'). The PCR products' sequencing was carried out by phylogeny analysis using MEGA 6 software. The amplicon value that succeeded in electrophoresis was 710bp, while six polymorphic sites were obtained at base positions of 1, 300, 379, 675, 676, and 679. The haplotypes (Hap) obtained were 4, with a genetic distance that ranged from 0.000-0.001. The nitrogenous bases of the amino acid composition from the samples showed no significant difference. The phylogenetic tree (Tamura-Nei) classified the cattle into two clades with a genetic distance of 0.0005.

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Phylogenetic analysis of the phoretic association between Bursaphelenchus conicaudatus (Nematoda: Aphelenchoididae) and Psacothea hilaris (Coleoptera: Cerambycidae)

Nematology ◽

10.1163/156854102760396566 ◽

2002 ◽

Vol 4 (6) ◽

pp. 759-771 ◽

Cited By ~ 13

Author(s):

Kazuyoshi Futai ◽

Natsumi Kanzaki

Keyword(s):

Phylogenetic Analysis ◽

Mitochondrial Dna ◽

Cytochrome Oxidase ◽

Molecular Analysis ◽

Phylogenetic Relationships ◽

Base Sequence ◽

Cytochrome Oxidase Subunit ◽

Cytochrome Oxidase Subunit I ◽

Oxidase Subunit ◽

Longicorn Beetle

AbstractThe phoretic relationships between the nematode Bursaphelenchus conicaudatus and the yellow-spotted longicorn beetle, Psacothea hilaris, collected at various districts in Japan were investigated. A high proportion of all ten subspecies of the beetle examined were infected with the nematodes, suggesting the universality of this phoretic association. Molecular analysis based on the partial base sequence of cytochrome oxidase subunit I in the mitochondrial DNA revealed that the phylogenetic relationships among ten subspecies of the beetle was similar to that among associated nematode isolates obtained from each beetle subspecies. Intraspecific vector replacements were supposed to occur in the isolates of B. conicaudatus on two islands. The phoretic association between the nematode and the beetle is assumed to have been established before the divergence of P.hilaris into subspecies.

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First record of body colour polymorphism in giant African snail Achatina fulica (Bowdich, 1822) - a comparative study using mitochondrial cytochrome oxidase subunit I (COI) gene

ENTOMON ◽

10.33307/entomon.v44i2.444 ◽

2019 ◽

Vol 44 (2) ◽

pp. 155-160

Author(s):

Keerthy Vijayan ◽

R. Sugantha Sakthivel ◽

T.V. Sajeev

Keyword(s):

Cytochrome Oxidase ◽

South India ◽

The Body ◽

Colour Polymorphism ◽

Coi Gene ◽

Body Colour ◽

Cytochrome Oxidase Subunit ◽

Cytochrome Oxidase Subunit I ◽

Black And White ◽

Oxidase Subunit

The presence of the body colour polymorphism in the tropical invasive pest giant African snail is reported for the first time from South India. Three different body colour polymorphs were recognised viz. grey, black and white. The grey body colour is the most common polymorph. The black and white colour polymorphs are found to be in almost equal proportions in the reported localities with the grey counterparts. The cytochrome oxidase subunit I (COI) sequences of the three colour polymorphs are found to be identical. The presence of the body colour polymorphism in south India may be attributed to the avian predation and other selection pressures.

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