scholarly journals Baseline susceptibility of Philippine Ostrinia furnacalis (Lepidoptera: Crambidae) populations to insecticidal Cry1A.105 and Cry2Ab2 proteins and validation of candidate diagnostic concentration for monitoring resistance

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Edwin Alcantara ◽  
MARNELLE M. ATIENZA ◽  
LUIS CAMACHO ◽  
SRINIVAS PARIMI
Keyword(s):  
Reference Strain ◽  
The Philippines ◽  
Ostrinia Furnacalis ◽  
Resistance Development ◽  
Upper Limit ◽  
Validation Experiment ◽  
Overlay Method ◽  
Insect Populations ◽  
Bioassay Data ◽  
Susceptibility Assay

Abstract. Alcantara EP, Atienza MM, Camacho L, Parimi S. 2021. Baseline susceptibility of Philippine Ostrinia furnacalis (Lepidoptera: Crambidae) populations to insecticidal Cry1A.105 and Cry2Ab2 proteins and validation of candidate diagnostic concentration for monitoring resistance. Biodiversitas 22: 956-960. This study estimated the baseline susceptibility of Ostrinia furnacalis populations from the Philippines, to purified insecticidal Cry1A.105 and Cry2Ab2 proteins and determined a diagnostic concentration (DC) through a validation experiment. The insect populations were collected from separate sites of corn farms in Northern and Central Luzon and in South Cotabato province of the island of Mindanao. Dose-response bioassays using artificial diet surface overlay method were conducted on eight populations. The bioassay results revealed that the LC50 of Cry1A.105 and Cry2Ab2 to O. furnacalis ranged from 0.03 ng/cm2 to 0.18 ng/cm2 and 1.40 ng/cm2 to 9.98 ng/cm2, respectively. The relative susceptibility ratios between the most susceptible and most tolerant populations were 6-fold for Cry1A.105 and about 7-fold for Cry2Ab2. The candidate diagnostic concentrations (DC) based on the LC99 were calculated using the baseline bioassay data for both Cry1A.105 and Cry2Ab2. The validation was performed on populations from the same locations used in the baseline susceptibility assay and a reference strain to produce at least 99% mortality for each protein. Data showed that populations tested with Cry1A.105 produced average mortality of at least 99% for the upper limit, while this was observed in the LC99 estimate for Cry2Ab2. The validated diagnostic concentration can be used for monitoring the resistance development of O. furnacalis exposed to Bt Corn, MON89034, in the Philippines.

scholarly journals Ultrastructure characters and partial mtDNA-COI haplotypes of Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae) from Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Ahmad Taufiq Arminudin ◽  
Suputa ◽  
Arman Wijonarko ◽  
Y. Andi Trisyono
Keyword(s):  
The Philippines ◽  
Coi Gene ◽  
Ostrinia Furnacalis ◽  
High Similarity ◽  
Microscopy Observation ◽  
Corn Borer ◽  
Mtdna Coi ◽  
Asian Corn Borer ◽  
Relationship Of ◽  
The Relationship

Abstract. Arminudin AT, Suputa, Wijonarko A, Trisyono YA. 2020. Ultrastructure characters and partial mtDNA-COI haplotypes of Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae) from Indonesia. Biodiversitas 21: 2914-2922. In addition to the confirmation of the species of corn borer, we analyzed the relationship of Ostrinia furnacalis in Indonesia with that in other countries. Moths of O. furnacalis were collected from several areas in Java and Sumatra islands of Indonesia. Forewing and labial palpi scales were investigated under Scanning Electron Microscopy observation. A 658 bp of O. furnacalis partial COI gene sequences of 1480-2138 nucleotides were downloaded from GenBank and BOLD system databases. The ridge lamellae and window characters as unique ultrastructure characters of scale were further supported by the COI gene analysis. High similarity was observed between the Java and the Philippines specimens, but they were a different haplotype compared with Sumatra’s specimen. The results could provide the baseline data on the genetic variation of O. furnacalis in Indonesia.

Insecticide resistance in the currant–lettuce aphid, Nasonovia ribisnigri (Hemiptera: Aphididae) in the UK

1999 ◽  
Vol 89 (1) ◽  
pp. 17-23 ◽  
Author(s):  
M.D. Barber ◽  
G.D. Moores ◽  
G.M. Tatchell ◽  
W.E. Vice ◽  
I. Denholm
Keyword(s):  
Insecticide Resistance ◽  
Gel Electrophoresis ◽  
Reference Strain ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aphis Gossypii ◽  
Nasonovia Ribisnigri ◽  
Resistant Strains ◽  
Bioassay Data ◽  
The Uk ◽  
Esterase Band

AbstractBioassay data for a reference strain of Nasonovia ribisnigri (Mosely), exhibiting similar responses to proven susceptible strains of Myzus persicae (Sulzer) and Aphis gossypii Glover, were used to assess insecticide resistance in two suspected resistant strains and nine field strains of N. ribisnigri originating from lettuce in the UK. Results showed widespread but varied levels of resistance to pirimicarb, lower and also varied resistance to pyrethroids and organophosphates, and no significant differences in response to imidacloprid. In some strains, resistance was associated with an intensely-staining esterase band disclosed by polyacrylamide gel electrophoresis (PAGE). However, no direct relationship between esterases and resistance has yet been established. There was no biochemical evidence of an altered acetylcholinesterase contributing to pirimicarb resistance in these strains.

scholarly journals Study the morphological charactertics and the effect of foods on development and growth of Ostrinia furnacalis (Guenee, 1854) (Lepidoptera: Pyralidae)

2018 ◽  
Vol 34 (1) ◽  
Author(s):  
Bui Minh Hong ◽  
Nguyen Duc Hung ◽  
Tran Dinh Chien
Keyword(s):  
Life Cycle ◽  
Agricultural Research ◽  
Average Length ◽  
Survival Rates ◽  
Mass Rearing ◽  
The Philippines ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Reproductive Ability ◽  
Complete Life Cycle

The effects of foods on the life cycle, survival rate, and reproductive ability of Ostrinia furnacalis (Guenee, 1854) were studied in laboratory. Maize HN88-Fed O. furnacalis had a complete life cycle of 32.9 ± 1.7 days, which was 38.4 ± 1.5 days shorter than those fed with maize LVN4. The average survival rates of O. furnacalis on two types of feeds were 97.5% and 94.4%, respectively. When O. furnacalis were fed with 100% honey or 50% sugar or water, the average length of female life cycle as well as their everage laid egg number were 11.1 ± 0.9 days and 535.2 ± 77.2 eggs/female; 9.9 ± 0.8 days and 371.7 ± 47.4 eggs/female; 7.3 ± 0.6 days and 216 ± 32.6 eggs/female, respectively. Keywords The effect of foods on Ostrinia furnacalis References Đặng Thị Dung (2003). Một số dẫn liệu về sâu đục thân ngô (Ostrinia furnacalis Guenee) Pyralidae-Lepidoptera trong vụ xuân 2003 tại Gia Lâm, Hà Nội, Tạp chí BVTV số 6, tr 7-12.[2]. Patanakamjorn Somporn (1975). Biology of the tropical corn borer, Ostrinia furnacalis (Guenee) inrelation to host plant resistance research. Retrospective Theses and Dissertations. 5435 [3]. Đặng Xuân Hưng (2010). Nghiên cứu một số đặc điểm sinh học, sinh thái của sâu đục thân ngô Ostrinia furnacalis Guenee và biện pháp phòng chống vụ đông 2009 và hè thu 2010 tại Gia Lâm, Hà Nội. Luận văn Thạc sỹ Nông nghiệp, Trường Đại học Nông nghiệp Hà Nội 75 trang.11/2010. [4]. Yoshio Hirai and Danilo M. Legacion (1985). Improvement of the Mass Rearing Techniques for the Asiatic Corn Borer, Ostrinia furnacalis (Guenee), in the Philippines. Japan Agricultural Research Quarterly (JARQ) Vol. 19, No. 3, 1985

Two mitochondrial lineages occur in the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae), in Japan

2008 ◽  
Vol 98 (5) ◽  
pp. 519-526 ◽  
Author(s):  
S. Hoshizaki ◽  
R. Washimori ◽  
S. Kubota ◽  
S. Ohno ◽  
Y. Huang ◽  
...  
Keyword(s):  
Population Expansion ◽  
The Philippines ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Preliminary Examination ◽  
Geographical Population ◽  
Asian Corn Borer ◽  
Geographical Regions ◽  
Genetic Features ◽  
Coii Gene

AbstractThe genealogy and diversity of the mitochondrial cytochrome oxidase subunit II (COII) gene were investigated for Ostrinia furnacalis in Japan. A preliminary examination of mitochondrial lineages in China and the Philippines was also made. Two lineages (A and B) were found in the COII gene. Lineage A was frequent throughout the Japanese main islands (Hokkaido, Honshu, Shikoku and Kyushu), while the frequency of lineage B varied among these islands. No clear patterns of geographical population structure were found. Population genetic features suggested that the O. furnacalis population harboring the lineage A mitochondria expanded in the recent past, while lineage B showed weak signals of a population expansion. It is not clear whether the two lineages of mtDNA evolved in separate or identical geographical regions. We discuss two hypotheses regarding the two lineages of mtDNA: a cryptic race/species hypothesis and a selective sweep hypothesis.

Disaster Medicine Training in the Philippines

2002 ◽  
Vol 17 (S2) ◽  
pp. S27
Author(s):  
Teodoro Javier Herbosa
Keyword(s):  
The Philippines ◽  
Disaster Medicine

How to Evaluate Remote Tower Metrics in Connection With Weather Observations

2018 ◽  
Vol 8 (2) ◽  
pp. 100-111 ◽  
Author(s):  
Maik Friedrich ◽  
Christoph Möhlenbrink
Keyword(s):  
Traffic Control ◽  
Air Traffic Control ◽  
Influential Factors ◽  
Air Traffic ◽  
Weather Event ◽  
Evaluation Of Performance ◽  
Weather Events ◽  
Weather Observations ◽  
Validation Experiment ◽  
Remote Controller

Abstract. Owing to the different approaches for remote tower operation, a standardized set of indicators is needed to evaluate the technical implementations at a task performance level. One of the most influential factors for air traffic control is weather. This article describes the influence of weather metrics on remote tower operations and how to validate them against each other. Weather metrics are essential to the evaluation of different remote controller working positions. Therefore, weather metrics were identified as part of a validation at the Erfurt-Weimar Airport. Air traffic control officers observed weather events at the tower control working position and the remote control working position. The eight participating air traffic control officers answered time-synchronized questionnaires at both workplaces. The questionnaires addressed operationally relevant weather events in the aerodrome. The validation experiment targeted the air traffic control officer’s ability to categorize and judge the same weather event at different workplaces. The results show the potential of standardized indicators for the evaluation of performance and the importance of weather metrics in relation to other evaluation metrics.

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