Analysis of Land Suitability for the Development of Porang (Amarphopallus ancophillus ) Plants in Lewa Sub-Regency, East Sumba Regency

Lewa sub-district is one of the sub-districts in East Sumba Regency, which has dry land that can be maximized for the development of porang plants and development purposes; information on the potential of porang plantations is needed. This study aimed to determine the biophysical characteristics of the land and the land suitability class of porang plants. The analytical method used was the matching method by comparing the land characteristics and plant growth requirements and the overlay method. The matching results show that the land characteristics in Lewa Subdistrict are class S1 (Very suitable), covering an area of 26.220,209 ha and Class S2 (quite suitable), covering an area of 3.608,523 ha. Limiting factors in this area are water availability (OA) such as drainage, nutrient retention (nr) such as CEC and pH, and erosion hazards (eh) such as slope.

Groundwater Vulnerability towards Pollution in Area Around the Piyungan Landfill, Bantul Regency, D. I. Yogyakarta

The Piyungan landfill is located in Bantul Regency, Daerah Istimewa Yogyakarta, still uses an open dumping system. The open dumping system produced more quantity of leachate from other systems. Leachate spreads to the ground and seeps into the ground to the groundwater surface. These conditions have an impact on the environment. This study aims to assess the groundwater vulnerability to leachate contamination in the area around the Piyungan landfill. The research method used is survey and mapping, and data analysis is carried out using the Le Grand method. Geographic Information System (GIS) is also used to visualize data into maps. The Le Grand method considers as many as 5 physical environmental parameters, including the groundwater depth, absorption above ground, aquifer permeability, groundwater slope, and horizontal distance of wells with pollutant sources. Next, scoring is carried out for each of these parameters, and then a groundwater vulnerability map is made using the overlay method. The results of the study were three classes of the potential vulnerability of groundwater pollutions. That were large pollution potential (may or may be polluted), medium pollution potential (maybe polluted but slightly), and small pollution potential (very difficult to pollute).

1276. Assessment of Anti-biofilm Activity of Staphylococcus aureus Bacteriophages Against Clinical Isolates from Patients with Left Ventricular Assist Device Infections

Background Staphylococcus aureus is a common cause of biofilm-mediated left ventricular device (LVAD) infections which are difficult to resolve with antibiotics alone and are associated with substantial morbidity and mortality. Recently, bacteriophages (phage) therapy has been used to resolve LVAD infections in a few cases. Our goal was to assess in vitro susceptibility and anti-biofilm activity of two S. aureus bacteriophages against clinical isolates from patients with S. aureus LVAD infections in order to develop a S. aureus phage cocktail for clinical use. Methods Two bacteriophages, OMS1 and OMS2, from the Israeli Phage Bank, were assessed for lytic activity against 15 S.aureus isolates (9 methicillin resistant, MRSA and 6 methicillin susceptible, MSSA) via agar overlay method and plaque forming units (PFU)/mL were enumerated. We then formed bacterial biofilms after overnight incubation at 120 rpm in a 96-well plates in duplicate; experiments were repeated thrice. Wells were then treated with tryptic soy broth (TSB, control) or TSB containing phage at 109 PFU/mL for 24 hours. After washing, biofilms were stained with crystal violet and biomass quantified via optical density at 570mm. Results All bacterial isolates were susceptible to both phages via agar overlay to varying degrees as determined by phage titers obtained via serial dilutions (Figure 1a, 1b). OMS1 led to significant reduction in biofilm of 7/9 MRSA and 3/6 MSSA isolates and OMS2 reduced biofilms in 9/9 MRSA and 4/6 MSSA isolates (Figure 1c). Phage titers and Biofilm Biomass Figure 1a) Phage titers in PFU/ml obtained from each bacterial isolate via agar overlay method. 1b) Representative agar plate demonstrating lytic plaques from OMS1 and OMS2 on a Staphylococcus aureus LVAD isolate. 1c) Biofilm biomass of Staphylococcus aureus isolates alone (SA) or with individual phage (SA+OMSA1 and SA=OMS2) assessed by optical density readings at 570 nm; error bars represent standard error of the mean. Conclusion We demonstrated in vitro lytic and anti-biofilm activity of 2 S. aureus phages against clinical S. aureus isolates from patients with LVAD infection. Our data suggests that phage susceptibility measured with agar overlay does not always correlate with phage susceptibility of S. aureus biofilms, suggesting that more than one method should be used to assess in vitro activity. We plan to assess for synergistic activity with the phage combination. Disclosures Ryan K. Shields, PharmD, MS, Shionogi (Consultant, Research Grant or Support) Ran Nir-Paz, MD, BiomX (Consultant)Technophage (Scientific Research Study Investigator, Advisor or Review Panel member) Saima Aslam, MD, MS, BioMx (Consultant)Cystic Fibrosis Foundation (Grant/Research Support)Gilead (Consultant)Johnson and Johnson (Consultant)Merck (Consultant)

Analisis Tingkat Kekumuhan Permukiman Pesisir Di Kecamatan Abeli

Abstrak: Penelitian ini bertujuan untuk menentukan indeks kekumuhan permukiman pesisir dan menganalisis kondisi eksisting permukiman di Kecamatan Abeli Kota Kendari dengan menggunakan analisis deskriptif kuantitatif. Penelitian ini menggunakan metode overlay dengan memberikan penilaian pada setiap indikator kekumuhan yang telah ditetapkan oleh Dirjen Perumahan dan Permukiman seperti: kepadatan bangunan, kelayakan bangunan, aksesibilitas lingkungan, drainase lingkungan, pelayanan air bersih, pengelolaan air limbah dan pengelolaan persampahan. Hasil penelitian menunjukkan bahwa indeks kekumuhan permukiman pesisir di Kecamatan Abeli terdiri dari dua kelas yaitu kumuh sedang dan kumuh berat. Kelas kumuh sedang dengan indeks kekumuhan 2,5-3,4 terdapat di Kelurahan Pudai RT 02, Kelurahan Lapulu RT 01 dan 02, Kelurahan Poasia RT 03 dan 05, Kelurahan Talia RT 01. Kelas kumuh berat dengan indeks kekumuhan 3,5- 4,4 terdapat pada Kelurahan Pudai RT 01, Kelurahan Lapulu RT 03 dan Kelurahan Talia RT 02, 03 dan 04. kondisi eksisting untuk tingkat permukiman kumuh sedang memiliki kondisi lingkungan umumnya cenderung menengah, dimana terdapat beberapa aspek yang memiliki kesamaan dengan permukiman kumuh ringan. Kondisi kumuh berat kondidsi lingkungannya buruk, terdapat kecenderungan bahwa semakin buruk tingkat kekumuhan permukiman, maka semakin buruk pula kondisinya dengan memilki kondisi sarana dan prasarana yang tidak memadai dan kondisi lingkungan yang kurang terjaga. Kata Kunci: permukiman kumuh, tingkat kekumuhan, overlay parameter Abstract: This study aims to determine the slum index of coastal settlements and analyze the existing conditions of settlements in Kendari City Abeli District using quantitative descriptive analysis. This study uses the overlay method by giving an assessment of each slum indicator set by the Director General of Housing and Settlements such as: building density, building feasibility, environmental accessibility, environmental drainage, clean water services, waste water management and waste management. The results showed that the slum index of coastal settlements in Abeli Subdistrict consisted of two classes namely medium slums and heavy slums. Where moderate slum with 2.5-3.4 slum index found in Pudai Village RT 02, Lapulu Village RT 01 and 02, Poasia Village RT 03 and 05, Talia Village RT 01. For heavy slum classes with 3.5- slum index 4.4 is found in Pudai RT 01 Village, Lapulu Village RT 03 and Talia RT 02, 03 and 04 Village. With the existing conditions for slum settlements having moderate environmental conditions generally tend to be medium, where there are several aspects that have similarities with light slum settlements . While the condition of heavy slum conditions is poor, there is a tendency that the worse the slum level of settlements, the worse the condition is by having inadequate conditions of facilities and infrastructure and adverse environmental conditions. Keywords: slum settlement, slum level, parameter overlay

Tipologi batas wilayah administrasi untuk memudahkan pengelolaan batas daerah di Indonesia

Abstrak Terhitung sejak 1945 – 2017, baru sekitar 48% dari 977 segmen batas daerah di Indonesia yang disahkan melalui Peraturan Menteri Dalam Negeri tentang batas daerah. Pengelolaan batas wilayah daerah sangat penting untuk berbagai urusan pembangunan misalnya pengelolaan sumber daya alam. Oleh karena itu, penelitian ini mengusulkan pendekatan baru untuk mendukung pengelolaan batas wilayah yang efisien yaitu melalui segmentasi berbasis tipologi batas wilayah. Tahapan analisis meliputi: membandingkan, memotong, dan mengelompokkan garis batas. Single Buffer Overlay Method digunakan untuk membandingkan unsur geografis pada Peta Rupabumi Indonesia (data referensi) dan garis batas 2017 (data yang diuji). Selanjutnya, dilakukan pemotongan garis sesuai hasil perbandingan. Pada akhirnya, garis tersebut dikelaskan berdasarkan tipologinya (igir, jalan, dan sungai) menggunakan metode SQL (Structured Query Language). Hasil penelitian menunjukkan bahwa mayoritas (41,8%) batas daerah di Indonesia tidak menggunakan unsur geografis tertentu sebagai penanda batasnya, sedangkan persentase penanda batas berupa sungai 35,9%, igir 16,4%, dan jalan 5,8%. Abstract Since 1945 – 2017, only 48% of 977 regional borderlines of Indonesia were legalized by The Ministry of Home Affairs. Properly managed intra-national boundaries are fundamental for development purposes e.g. natural resource management. Therefore, this research proposed a new approach to help managing the boundary efficiently through typology-based borderlines segmentation which was conducted by some stages: compare, split, and classify lines. Single Buffer Overlay Method used for comparison purpose by utilizing some geographical features on a topographic map as a referenced dataset and boundary line (2017 database) as a tested dataset. Then we split the lines based on the comparison result. Finally, each split line was classified into border typologies (road, ridge, and stream) by using the SQL (Structured Query Language) method. We found that most of The Indonesian administrative boundary segments (41.8%) did not use a geographical feature, while the boundary on the rivers 35.9%, ridges 16.4%, and roads 5.8%.

Pemetaan Tingkat Kerawananan Banjir Di Kota Jambi Menggunakan Metode Scoring Dan Overlay Berbasis Sistem Informasi Geografis

This research is about flood vulnerability mapping in Jambi City based on Geographic Information System (GIS). This study is aiming to find out the flood vulnerability level, spatial distribution of flood, and flood prone areas in Jambi City. We used five parameters that affect flood vulnerability, including land slope, land level, land use, soil type, and rainfall during 2019. The method used is the scoring and overlay method with the help of ArcGis software. Flood vulnerability level was divided into three categories, namely quite vulnerable, vulnerable, and very vulnerable. The results obtained in this study are that most of Jambi City has a level of flood vulnerability in the vulnerable category, which is an area of 9254.82 ha (58%), while for the area that is dominated quite safe from flooding, Jambi Selatan sub-district, is 2849.14 ha (18%). This shows that more than half of the Jambi city area is a flood-prone area so it is very important to carry out structural and non-structural mitigation actions

Analisis Tingkat Kerawanan Banjir di Kabupaten Semarang Menggunakan Overlay dan Skoring Memanfaatkan SIG

Abstrak: Bencana banjir merupakan bencana yang sering terjadi di wilayah iklim tropis khususnya daerah yang letaknya dikelilingi oleh aliran air atau genangan air seperti danau. Banjir di Indonesia dipengaruhi oleh pergantian musim yang tidak menentu yang menyebabkan bencana banjir. Penelitian ini bertujuan untuk memetakan wilayah yang rawan terhadap banjir dan mengetahui sebarapa besar tingkat kerawananan banjir yang dapat terjadi di studi area penelitian pada wilayah Kabupaten Semarang. Penelitian ini menggunakan metode overlay dengan tumpang susun layer, berdasarkan hasil perhitungan total scoring dari nilai dan bobot dari tiap parameter. Pengumpulan data dilakukan dengan mengumpulkan data sekunder berupa data spasial dan non spasial dari lembaga resmi Negara Indonesia, yang dipakai untuk mendukung analisis yang dilakukan. Dari hasil analisis yang dilakukan pada studi area penelitian di wilayah Kabupaten Semarang, diketahui terdapat 159 desa pada daerah dengan tingkat kerawanan yang rendah, serta 109 desa pada daerah dengan tingkat kerawanan sedang, sisanya 20 desa berada pada tingkat kerawanan tinggi. Dari analisis yang dilakukan, dapat ditarik kesimpulan bahwa wilayah Kabupaten Semarang khususnya pada daerah studi area penelitian menghasilkan informasi dari empat kecamatan yang ada, memiliki tingkat kerentanan yang didominasi oleh tingkat tidak rawan dan cukup rawan. Kata kunci: Bencana Banjir, Overlay, Scoring, Sistem Informasi Geografi.Abstract: Disaster flood disasters that occur in climatic areas, especially areas associated with water flows or puddles such as lakes. Floods in Indonesia are inspired by the changing seasons that are disastrous floods. This study aims to map the areas that are prone to flooding and how much danger is the level of flood vulnerability that can occur in the study area of Semarang Regency. This study uses an overlay method with overlapping layers, based on the calculation of the total score of the values and weights of each parameter. Data collection was carried out by collecting secondary data in the form of spatial data and non-official Indonesian institutions, which were used to support the analysis carried out. From the results of the analysis carried out in the study area of research in Semarang Regency, it is known that there are 159 villages in areas with low levels of vulnerability, as well as 109 villages in areas with moderate levels of vulnerability, the remaining 20 villages are at high levels of vulnerability. From the analysis carried out, it can be concluded that the Semarang Regency area, especially in the study area the research area produces information from the four existing sub-districts, has a level of vulnerability which is dominated by a level of vulnerability which is not vulnerable and quite vulnerable. Keywords: Flood Disaster, Overlay, Scoring, Geographic Information System.