scholarly journals Application of wheat flour (Triticum aestivum) on spore density and sporulation efficiency of Bacillus megaterium isolated from Litopenaeus vannamei gastrointestinal tract

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
I MADE DEDI MAHARIAWAN ◽  
WAHYU ENDRA KUSUMA ◽  
ATING YUNIARTI ◽  
M.A.G. BELTRAN ◽  
ANIK MARTINAH HARIATI

Abstract. Mahariawan IMD, Kusuma WE, Yuniarti A, Beltran MAG, Hariati AM. 2021. Application of wheat flour (Triticum aestivum) on spore density and sporulation efficiency of Bacillus megaterium isolated from Litopenaeus vannamei gastrointestinal tract. Biodiversitas 22: 3709-3715. Bacillus megaterium is frequently used in fish farming, such as white shrimp (Litopenaeus vannamei) pond, which can produce spores with high stability in its implementation. Currently, spore production still requires the availability of high-cost carbon sources. The objective of this research was to evaluate the effect of different wheat flour doses on spore density and sporulation efficiency of B. megaterium BM1. In flasks, 50 mL of each test medium was treated with different doses of wheat (10, 20, 30 and 40 g. L-1, respectively) and glucose was used as a control. Each treatment was inoculated with B. megaterium BM1 (2.6 x 108 CFU. mL-1) and incubated in a shaker incubator (120 rpm) at 37 °C for 120 hours. The results showed that the highest vegetative cell concentration (17 x 108 CFU. mL-1), growth rate (0.8 hour-1) and spore (14.7 x 108 spores. mL-1) were found in the wheat flour dose of 30 g. L-1. Furthermore, the highest sporulation efficiency was achieved at 20 g. L-1 of wheat (91.30%) and germination should be done at a dose less than 40 g. L-1. The size of the spores was 1.35-1.39 µm. Thus, 30 g. L-1 of wheat flour is a potential dose to produce spore for probiotic candidates.  

2021 ◽  
Vol 16 (2) ◽  
pp. 107
Author(s):  
Erlangga Erlangga ◽  
Cut Nuraini ◽  
Salamah Salamah

Udang vaname (Litopenaeus vannamei) merupakan spesies udang introduksi yang sudah banyak dibudidayakan di tambak di Indonesia. Permasalahan pada budidaya udang vaname di tambak dengan padat tebar tinggi dan penggunaan pakan protein tinggi adalah tingginya akumulasi residu/limbah budidaya. Salah satu cara memanfaatkan limbah budidaya yaitu sistem heterotrof dengan menggunakan teknologi bioflok dengan memanipulasi rasio perbandingan karbon nitrogen (C/N ratio) di dalam media budidaya. Penelitian ini bertujuan untuk mengevaluasi pembentukan flok pada pemeliharaan udang vaname dengan pemberian sumber karbon yang berbeda. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap yang terdiri atas lima perlakuan, yaitu: kontrol, molase, tepung terigu, tepung maizena, dan air tebu; masing-masing tiga ulangan. Setiap perlakuan diberikan pada wadah pemeliharaan udang vaname untuk menumbuhkan flok dengan menambahkan probiotik komersil. Analisis data yang dilakukan antara lain pertumbuhan dan sintasan udang, FCR, ukuran flok, volume flok, kandungan gizi flok, dan parameter kualitas air yang mendukung kehidupan udang vaname. Hasil penelitian menunjukkan bahwa pemberian sumber karbon berbeda memberikan pengaruh nyata terhadap pertumbuhan udang. Pemberian tepung terigu dalam pembentukan flok merupakan sumber karbon yang terbaik untuk meningkatkan pertumbuhan udang vaname dengan pertambahan bobot 0,56 g; panjang 1,96 cm; dan sintasan 90,67%; dengan nilai FCR 1,10; kandungan protein flok sebesar 27,15%; ukuran flok 450 mikron; dan volume flok 88 mL/L. Nilai kisaran parameter kualitas air DO 5,5-6,5 mg/L; pH 6,8-8,0; suhu 26°C-30°C; salinitas 30-33 ppt; dan amonia 0,1-1,54 mg/L. Implikasi penelitian ini membuktikan pemberian sumber karbon memberikan pengaruh terhadap peningkatan flok dan mampu meningkatkan pertumbuhan dan sintasan udang vaname. Sebaiknya diperlukan penelitian lebih spesifik untuk mencari dosis terbaik dan maksimal dari sumber karbon tepung terigu untuk pembentukan flok, pertumbuhan, dan sintasan udang vaname.Vannamei shrimp (Litopenaeus vannamei) is an introduced species of shrimp that has been widely farmed in brackishwater ponds in Indonesia. Vannamei shrimp farmed in ponds with high stocking density and fed with high protein feed produce large quantities of residues/waste. Reducing the waste could be achieved by using biofloc technology to manipulate the carbon nitrogen ratio (C/N ratio) in the culture media. This study aimed to evaluate the formation of flocks grown on different carbon sources in the rearing media of vannamei shrimp. The study used an experimental method with a completely randomized design consisting of five treatments, namely: control, molasses, wheat flour, corn starch, and sugarcane juice, each with three replications. Data analysis was carried out on shrimp growth and survival, FCR, floc size, floc volume, floc nutrient content, and water quality parameters that support the life of vannamei shrimp. The results showed that different carbon sources had a significant effect on the shrimp growth. Flour is the best source of carbon to support the formation of flocks which increases the growth of vannamei shrimp with a weight gain of 0.56 g, a length of 1.96 cm, and a survival rate of 90.67%, with an FCR value of 1.10, a floc protein content of 27.15%, a floc size of 450 microns, and a floc volume of 88 mL/L. The measured variations of DO, pH, temperature, salinity, and and ammonia were 5.5-6.5 mg/L, 6.8-8.0, 26°C-30°C, 30-33 ppt, and 0.1-1.54 mg/L, respectively. This research demonstrates that the provision of different carbon sources has an effect on increasing flocks and are able to increase the growth and survival of vannamei shrimp. It is recommended that more specific research is needed to find the best and maximum dose of wheat flour carbon sources for floc formation, growth and survival of vannamei shrimp.


2016 ◽  
Vol 48 (4) ◽  
pp. 1491-1501 ◽  
Author(s):  
Mohammad Hossein Khanjani ◽  
Mir Masoud Sajjadi ◽  
Morteza Alizadeh ◽  
Iman Sourinejad

2010 ◽  
Author(s):  
Zhi-Qiang Du ◽  
Suneel K. Onteru ◽  
Danielle Gorbach ◽  
Max F. Rothschild

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