Age-Related Osteogenic Potential of Mesenchymal Stromal Stem Cells from Human Vertebral Bone Marrow

Aging and sedentary lifestyle are common nowadays and are associated with the increasing number of chronic diseases. Thus, physical activity is recommended as one of three healthy behavior factors that play a crucial role in health prophylaxis. In the present study, we were interested whether physical activity influences the number and potential of bone-marrow-derived mesenchymal stem cells BMMSCs. In this study, four-week-old male C57Bl/6 mice were trained on a treadmill at progressive speeds over a 5-week period. Comparisons made between exercised (EX) and sedentary animal groups revealed (i) significantly higher number of MSCs in EX animals, (ii) elevated alkaline phosphatase (ALP) activity, (iii) increased level of osteopontin (OPN) and osteocalcin (OCL), and (iv) reduced marrow cavity fat. The results obtained support the thesis that EX may play a substantial role in the regeneration of mesenchymal tissues. Therefore, EX may represent a novel, nonpharmacological strategy of slowing down age-related decline of the musculoskeletal functions.

Download Full-text

Age-related CXC chemokine receptor-4-deficiency impairs osteogenic differentiation potency of mouse bone marrow mesenchymal stromal stem cells

The International Journal of Biochemistry & Cell Biology ◽

10.1016/j.biocel.2013.05.034 ◽

2013 ◽

Vol 45 (8) ◽

pp. 1813-1820 ◽

Cited By ~ 25

Author(s):

Liang G. Guang ◽

Adele L. Boskey ◽

Wei Zhu

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Osteogenic Differentiation ◽

Chemokine Receptor ◽

Mouse Bone Marrow ◽

Cxc Chemokine Receptor 4 ◽

Age Related ◽

Cxc Chemokine ◽

Chemokine Receptor 4 ◽

Stromal Stem Cells

Download Full-text

Alpha-ketoglutarate ameliorates age-related osteoporosis via regulating histone methylations

Nature Communications ◽

10.1038/s41467-020-19360-1 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Yuan Wang ◽

Peng Deng ◽

Yuting Liu ◽

Yunshu Wu ◽

Yaqian Chen ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Therapeutic Potential ◽

Embryonic Stem ◽

Tca Cycle ◽

Bmp Signaling ◽

Osteogenic Potential ◽

Aged Mice ◽

Age Related

Abstract Age-related osteoporosis is characterized by the deterioration in bone volume and strength, partly due to the dysfunction of bone marrow mesenchymal stromal/stem cells (MSCs) during aging. Alpha-ketoglutarate (αKG) is an essential intermediate in the tricarboxylic acid (TCA) cycle. Studies have revealed that αKG extends the lifespan of worms and maintains the pluripotency of embryonic stem cells (ESCs). Here, we show that the administration of αKG increases the bone mass of aged mice, attenuates age-related bone loss, and accelerates bone regeneration of aged rodents. αKG ameliorates the senescence-associated (SA) phenotypes of bone marrow MSCs derived from aged mice, as well as promoting their proliferation, colony formation, migration, and osteogenic potential. Mechanistically, αKG decreases the accumulations of H3K9me3 and H3K27me3, and subsequently upregulates BMP signaling and Nanog expression. Collectively, our findings illuminate the role of αKG in rejuvenating MSCs and ameliorating age-related osteoporosis, with a promising therapeutic potential in age-related diseases.

Download Full-text