Effect of FGF-2 on Collagen Tissue Regeneration by Human Vertebral Bone Marrow Stem Cells

Abstract Background Patients with type 2 diabetes mellitus (T2DM), especially those with poor glycemic control, are characterized by low bone mass and destruction of bone microstructure. Nowadays, autologous mesenchymal stem cells (auto-MSCs) have been used to repair defects and promote tissue regeneration due to handy source, low immunogenicity and self-renewing and multi-differentiating potential. However, T2DM changed the biological properties of auto-MSCs, and investigating the most suitable auto-MSCs for T2DM patients becomes a focus in tissue engineering. Results In this research, we compared the biological characteristics of adipose-derived stem cells (ASCs) and bone marrow stem cells (BMSCs) derived from T2DM rats. These results demonstrated that ASCs had a higher proliferation rate, colony-formation and cell-sheet forming ability, while BMSCs got better osteogenesis-related staining, expression of osteogenesis-related genes and proteins, and osteogenic capacity in vitro. Conclusions As it turned out, ASCs from T2DM had a higher proliferation, while BMSCs had significantly higher osteogenetic ability no matter in vitro and in vivo. Therefore, we should take into account the specific and dominated properties of MSC according to different needs to optimize the protocols and improve clinical outcomes for tissue regeneration of T2DM patients.

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TRANSPLANTATION OF GOLD NANOPARTICLES WITH OR WITHOUT BONE MARROW STEM CELLS FOR REGENERATION OF SURGICALLY INDUCED TONGUE DEFECT IN RATS

International Journal of Advanced Research ◽

10.21474/ijar01/13850 ◽

2021 ◽

Vol 9 (11) ◽

pp. 1131-1136

Author(s):

Eman Azmy ◽

◽

Mona Denewar ◽

Rehab R. El-Zehary ◽

Fatma M. Ibrahim ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Tissue Regeneration ◽

Donor Site ◽

Fibrous Tissue ◽

Donor Site Morbidity ◽

Bone Marrow Stem Cells ◽

Tongue Reconstruction ◽

Reconstructive Technique ◽

Surgically Induced

The ultimate goal of tongue reconstruction is restoring the tongue integrity while preserve its critical functions(i.e., articulation, mastication, and deglutition) and minimize the morbidity profile of a selected reconstructive technique. Mesenchymal stem cells (MSCs) used in tissue engineering became a therapeutic selectiondue to numerous advantagesas regeneration of damaged tissues withhigh quality without the formation of fibrous tissue, minimal donor site morbidity in comparison withautografts and a low risk ofdisease transmition and autoimmune rejection. Bone marrow stem cells (BMSCs) areexpandable stem, self-renewing,embedded at the site of injury and motivate tissue regeneration and wound healing. In oral wounds, they revealgreater re-epithelialization, intracellular matrix formation,cellularity, and neoangiogenesis, thusspeed up wound healing.Gold nanoparticles (GNPs) application in medical fields is growingdue to the chemical and physical properties like biocompatibility, optical properties,facile surface modification and stability.GNP usage in regenerative medicine is safe if the implanted tissue is replacing a tissue/organresectedbecause of tumor.

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