Laser speckle contrast imaging with extended depth of field for in-vivo tissue imaging

Abstract Background Compromised perfusion due to ligation of arteries and veins in esophagectomy with gastric tube reconstruction often (5–20%) results in necrosis and anastomotic leakage, which relate to high morbidity and mortality (3–4%). Ephedrine is used widely in anesthesia to treat intra-operative hypotension and may improve perfusion by the increase of cardiac output (CO) and mean arterial pressure (MAP). This study tests the effect of ephedrine on perfusion of the future anastomotic site of the gastric conduit, measured by Laser Speckle Contrast Imaging (LSCI). Methods This prospective, observational, in-vivo pilot study includes 26 patients undergoing esophagectomy with gastric tube reconstruction from October 2015 to June 2016 in the Academic Medical Center (Amsterdam). Perfusion of the gastric conduit was measured with LSCI directly after reconstruction and after an increase of MAP by ephedrine 5 mg. Perfusion was quantified in flux (LSPU) in four perfusion locations, from good perfusion (base of the gastric tube) towards decreased perfusion (fundus). Intra-patient differences before and after ephedrine in terms flux were statistically tested for significance with a paired t-test. Results LSCI was feasible to image gastric microcirculation in all patients. Flux (LSPU) was significantly higher in the base of the gastric tube (791 ± 442) compared to the fundus (328 ± 187) (P < 0.001). After administration of ephedrine, flux increased significantly in the fundus (P < 0·05) measured intra-patients. Three patients developed anastomotic leakage. In these patients, the difference between measured flux in the fundus compared to the base of the gastric tube was high. Conclusion This study presents the effect of ephedrine on perfusion of the gastric tissue measured with LSCI in terms of flux (LSPU) after esophagectomy with gastric tube reconstruction. We show a small but significant difference between flux measured before and after administration of ephedrine in the future anastomotic tissue (313 ± 178 vs. 397 ± 290). We also show a significant decrease of flux towards the fundus. Disclosure All authors have declared no conflicts of interest.

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Learning of speckle statistics for in vivo and noninvasive characterization of cutaneous wound regions using laser speckle contrast imaging

Microvascular Research ◽

10.1016/j.mvr.2016.04.008 ◽

2016 ◽

Vol 107 ◽

pp. 6-16 ◽

Cited By ~ 5

Author(s):

Kausik Basak ◽

Goutam Dey ◽

Manjunatha Mahadevappa ◽

Mahitosh Mandal ◽

Debdoot Sheet ◽

...

Keyword(s):

Laser Speckle ◽

Contrast Imaging ◽

Laser Speckle Contrast Imaging ◽

Cutaneous Wound ◽

Speckle Contrast ◽

Noninvasive Characterization

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Effect of ephedrine on gastric conduit perfusion measured by laser speckle contrast imaging after esophagectomy: a prospective in vivo cohort study

Diseases of the Esophagus ◽

10.1093/dote/doy031 ◽

2018 ◽

Vol 31 (10) ◽

Cited By ~ 4

Author(s):

S M Jansen ◽

D M de Bruin ◽

M I van Berge Henegouwen ◽

P R Bloemen ◽

S D Strackee ◽

...

Keyword(s):

Cohort Study ◽

Gastric Conduit ◽

Laser Speckle ◽

Contrast Imaging ◽

Laser Speckle Contrast Imaging ◽

Speckle Contrast

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IN VIVO MAPPING BRAIN MICROCIRCULATION BY LASER SPECKLE CONTRAST IMAGING: A MAGNETIC RESONANCE PERSPECTIVE OF THEORETICAL FRAMEWORK

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545808000236 ◽

2008 ◽

Vol 01 (02) ◽

pp. 239-256 ◽

Cited By ~ 1

Author(s):

ZHENG WANG

Keyword(s):

Magnetic Resonance ◽

Functional Neuroimaging ◽

Spatial Scales ◽

Laser Speckle ◽

Biophysical Model ◽

Future Research ◽

Contrast Imaging ◽

Laser Speckle Contrast Imaging ◽

Speckle Contrast

The fundamental limitations of most vascular-based functional neuroimaging techniques are placed by the fact how fine the brain regulates the blood supply system. In vivo mapping of the cerebral microcirculation with high resolution and sensitivity hence becomes unprecedentedly compelling. This paper reviews the theoretical background of the laser speckle contrast imaging (LSCI) technique and attempts to present a complete framework stemming from a simple biophysical model. Through the sensitivity analysis, more insights into the tool optimization are attained for in vivo applications. Open questions of the technical aspects are discussed within this unified framework. Finally, it concludes with a brief perspective of future research in a way analogous to the magnetic resonance imaging (MRI) technique. Such exploration could catalyze their development and initiate a technological fusion for precise assessment of blood flow across various spatial scales.

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