Controlling the Emission of Organic Dyes for High Sensitivity and Super-Resolution Microscopy

2009 ◽  
Author(s):  
Thorben Cordes ◽  
Ingo H. Stein ◽  
Carsten Forthmann ◽  
Christian Steinhauer ◽  
Monika Walz ◽  
...  
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
High Sensitivity ◽  
Super Resolution Microscopy

Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy

2009 ◽  
Author(s):  
Thorben Cordes ◽  
Ingo H. Stein ◽  
Carsten Forthmann ◽  
Christian Steinhauer ◽  
Monika Walz ◽  
...  
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
High Sensitivity ◽  
Super Resolution Microscopy

Novel organic dyes for multicolor localization-based super-resolution microscopy

2015 ◽  
Vol 9 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Martin Lehmann ◽  
Gregor Lichtner ◽  
Haider Klenz ◽  
Jan Schmoranzer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Fluorescent Probes for STED Optical Nanoscopy

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 21
Author(s):  
Sejoo Jeong ◽  
Jerker Widengren ◽  
Jong-Chan Lee
Keyword(s):  
Fluorescent Probes ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Photophysical Properties ◽  
Optical Resolution ◽  
Super Resolution ◽  
Fluorescent Nanoparticles ◽  
Resolution Limit ◽  
Photochemical Properties ◽  
Super Resolution Microscopy

Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy. Super-resolution microscopy, or optical nanoscopy, overcame the far-field optical resolution limit, known as Abbe’s diffraction limit, by taking advantage of the photophysical properties of fluorescent probes. Therefore, fluorescent probes for super-resolution microscopy should meet the new requirements in the probes’ photophysical and photochemical properties. STED optical nanoscopy achieves super-resolution by depleting excited fluorophores at the periphery of an excitation laser beam using a depletion beam with a hollow core. An ideal fluorescent probe for STED nanoscopy must meet specific photophysical and photochemical properties, including high photostability, depletability at the depletion wavelength, low adverse excitability, and biocompatibility. This review introduces the requirements of fluorescent probes for STED nanoscopy and discusses the recent progress in the development of fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, for the STED nanoscopy. The strengths and the limitations of the fluorescent probes are analyzed in detail.

scholarly journals Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy

2021 ◽  
Vol 8 ◽  
Author(s):  
Hao Dong ◽  
Ling-Dong Sun ◽  
Chun-Hua Yan
Keyword(s):  
Cell Biology ◽  
Organic Dyes ◽  
Energy Levels ◽  
Super Resolution ◽  
Upconversion Nanoparticles ◽  
Non Invasive ◽  
Optimal Resolution ◽  
Resolution Imaging ◽  
Anti Stokes ◽  
Super Resolution Microscopy

Super-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real energy levels, lanthanide-doped upconversion nanoparticles are featured by unique optical properties including excellent photostability, large anti-Stokes shifts, multicolor narrowband emissions, tunable emission lifetimes, etc. The past few years have witnessed the development of upconversion nanoparticles as probes for super-resolution imaging studies. To date, the optimal resolution reached 28 nm (λ/36) for single nanoparticles and 82 nm (λ/12) for cytoskeleton structures with upconversion nanoparticles. Compared with conventional probes such as organic dyes and quantum dots, upconversion nanoparticle-related super-resolution microscopy is still in the preliminary stage, and both opportunities and challenges exist. In this perspective article, we summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field. This perspective article should be enlightening for designing efficient upconversion nanoprobes for super-resolution imaging and promote the development of upconversion nanoprobes for cell biology applications.

Photoblueing of organic dyes can cause artifacts in super-resolution microscopy

2021 ◽  
Vol 18 (3) ◽  
pp. 253-257
Author(s):  
Dominic A. Helmerich ◽  
Gerti Beliu ◽  
Siddharth S. Matikonda ◽  
Martin J. Schnermann ◽  
Markus Sauer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

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