Non-destructive, label-free metabolic mapping during stem cell differentiation

2013 ◽  
Author(s):  
Kyle P. Quinn ◽  
Gautham V. Sridharan ◽  
Rebecca S. Hayden ◽  
David L. Kaplan ◽  
Kyongbum Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Label Free ◽  
Metabolic Mapping ◽  
Non Destructive

scholarly journals Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

2021 ◽  
pp. 2103955
Author(s):  
Nishir Mehta ◽  
Shahensha Shaik ◽  
Alisha Prasad ◽  
Ardalan Chaichi ◽  
Sushant P. Sahu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Label Free

scholarly journals Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

2020 ◽  
Author(s):  
Nishir Mehta ◽  
Shahensha Shaik ◽  
Alisha Prasad ◽  
Ardalan Chaichi ◽  
Sushant P. Sahu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Adipogenic Differentiation ◽  
High Sensitivity ◽  
Stem Cell Differentiation ◽  
Metabolic Imaging ◽  
Spectral Signature ◽  
Ionization Mass ◽  
Label Free

ABSTRACTStem cell-based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective therapy requires precise non-destructive evaluation of the purity of stem cells with high sensitivity (< 0.001% of the number of cells). Here, we report a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping (SAM)-based machine learning analysis to distinguish differentiating human adipose derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enabled identification of differentiated cells at single cell resolution. The label-free HSI method was compared with the standard methods such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. Further, we performed Raman microscopy and multiphoton-based metabolic imaging to provide complimentary information for the functional imaging of the hASCs. Finally, the HSI method was validated using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label-free identification of stem cell differentiation with high spatial and chemical resolution. This could provide a powerful tool to assess the safety and efficacy of stem cell-based regenerative therapies.

Micro-Engineered Sythetical Extrocellular Metrix for Stem Cell Differentiation Study

2010 ◽  
Author(s):  
Jianping Fu
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Cell Morphology ◽  
Focal Adhesions ◽  
Stem Cell Differentiation ◽  
Material Surface ◽  
Single Cell Level ◽  
Cell Level ◽  
Substrate Rigidity ◽  
Non Destructive

We report the use of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrate that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility, and stem cell differentiation. Furthermore, these micropost arrays reveal that changes in cytoskeletal contractility can precede stem cell differentiation and be utilized as a non-destructive predictor for fate decisions at the single cell level.

scholarly journals Label-free biomarkers of human embryonic stem cell differentiation to hepatocytes

2016 ◽  
Vol 89 (6) ◽  
pp. 575-584 ◽  
Author(s):  
Dimitrios Tsikritsis ◽  
Hu Shi ◽  
Yuan Wang ◽  
Srinivas Velugotla ◽  
Vlastimil Sršeň ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Label Free

Mechanical Regulation of Stem Cell Differentiation on Geometrically Modulated Elastomeric Substrates

2010 ◽  
Author(s):  
Jianping Fu
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Cell Morphology ◽  
Focal Adhesions ◽  
Stem Cell Differentiation ◽  
Material Surface ◽  
Single Cell Level ◽  
Cell Level ◽  
Substrate Rigidity ◽  
Non Destructive

We report the use of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrate that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility, and stem cell differentiation. Furthermore, these micropost arrays reveal that changes in cytoskeletal contractility can precede stem cell differentiation and be utilized as a non-destructive predictor for fate decisions at the single cell level.

In situ label-free monitoring of human adipose-derived mesenchymal stem cell differentiation into multiple lineages

Biomaterials ◽  
2018 ◽  
Vol 154 ◽  
pp. 223-233 ◽  
Author(s):  
Intan Rosalina Suhito ◽  
Yoojoong Han ◽  
Junhong Min ◽  
Hyungbin Son ◽  
Tae-Hyung Kim
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Label Free ◽  
Mesenchymal Stem Cell Differentiation
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